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Organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold as well as preparation method and application thereof

A tissue engineering scaffold and organic nanofiber technology, applied in the medical field, can solve problems such as poor mechanical strength, improve mechanical properties, facilitate ingrowth and proliferation, and promote blood vessel regeneration.

Pending Publication Date: 2022-08-09
诺一迈尔(苏州)生命科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of poor mechanical strength of bioceramic materials, an organic / inorganic composite three-dimensional porous nanofiber tissue engineering scaffold and its preparation method and application are provided

Method used

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  • Organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold as well as preparation method and application thereof
  • Organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold as well as preparation method and application thereof
  • Organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] SiO 2 Preparation of Nanofibrous Membranes:

[0030]Ethyl orthosilicate, deionized water, and phosphoric acid are mixed in a molar ratio of 1:11:0.01, and stirred for 30 hours to obtain a silica sol solution; PVA and deionized water are mixed in a mass ratio of 1:9, and then heated and stirred until PVA is completely dissolved to form a uniform PVA aqueous solution with a mass fraction of 10%;

[0031] The first electrospinning solution was obtained by mixing the above-mentioned silica sol solution with the above-mentioned PVA aqueous solution, adding it into a syringe, installing it on a propelling pump, connecting the high voltage and setting the electrospinning high voltage to be 20kV, and the propelling speed to be 1mL / h, Aluminum foil was used as the receiving device, and the receiving distance was 20 cm, and SiO was obtained by electrospinning. 2 / PVA nanofiber membrane;

[0032] the SiO 2 / PVA nanofiber membrane was calcined at 800 °C for 2 h to obtain pure S...

Embodiment 2

[0035] Preparation of PLLA / Gelatin nanofiber membranes:

[0036] Gelatin and PLLA were mixed and dissolved in hexafluoroisopropanol at a mass ratio of 5:1 (the total mass of gelatin and PLLA dissolved in hexafluoroisopropanol per milliliter was 0.1 g) to form a second electrospinning method. Silk liquid, add it to the syringe, install it on the propelling pump, connect the high voltage, set the high voltage for electrospinning to 15kV, the propelling speed to 3mL / h, the aluminum foil as the receiving device, the receiving distance to be 10cm, and electrospinning to obtain PLLA / Gelatin nanofiber membrane.

Embodiment 3

[0038] Preparation of organic / inorganic composite three-dimensional porous nanofibrous tissue engineering scaffolds:

[0039] Take the SiO in Example 1 2 A total of 2.5 g of the nanofiber membrane and the PLLA / Gelatin nanofiber membrane in Example 2 formed mixed nanofibers, in which SiO 2 The proportion of the nanofiber membrane to the total mass of the mixed nanofibers is 0%, 20%, 40%, 60%, and the mixture is cut into pieces of 1cm×1cm size;

[0040] Add 2.5g of mixed nanofibers to 100mL of tert-butanol, and use a high-speed homogenizer to homogenize and smash into a uniform mixed nanofiber solution;

[0041] Pour the mixed short nanofiber solution into the mold, freeze it in a -80°C refrigerator for 24 hours, then take it out and place it in a freeze dryer for 3 days of freeze drying to obtain an uncrosslinked organic / inorganic composite nanofiber tissue;

[0042] The cross-linked organic / inorganic composite nanofibrous tissue was placed in a muffle furnace and heated to 1...

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Abstract

The invention relates to an organic / inorganic composite three-dimensional porous nanofiber tissue engineering scaffold as well as a preparation method and application thereof.The preparation method comprises the following steps: (1) mixing a silicon source, water and acid to prepare a silicon dioxide sol solution, then mixing the silicon dioxide sol solution with a polyvinyl alcohol aqueous solution to obtain a first electrostatic spinning solution, and then performing electrostatic spinning to obtain a second electrostatic spinning solution; calcining to obtain a silicon dioxide nanofiber membrane; (2) dissolving gelatin and polylactic acid in an organic solvent to form a second electrostatic spinning solution, and then performing electrostatic spinning to prepare an organic nanofiber membrane; and (3) adding the silicon dioxide nanofiber membrane and the organic nanofiber membrane into an alcohol solvent, then carrying out homogenate crushing and freeze drying to obtain an uncrosslinked organic / inorganic composite nanofiber tissue, and then carrying out thermal crosslinking to obtain the organic / inorganic composite three-dimensional porous nanofiber tissue engineering scaffold. And when being applied to bone tissue repair, the material is beneficial to uniform spreading of cells and promotes bone tissue regeneration.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an organic / inorganic composite three-dimensional porous nanofiber tissue engineering scaffold and a preparation method and application thereof. Background technique [0002] Bone is the hardest organ in the human body, composed of bone tissue, periosteum and bone marrow. As an important tissue organ of the human body, bone performs functions such as structural support, protection, exercise lever, hematopoiesis, and storage of mineral ions, and is responsible for various movements of the human body together with the attached muscle tissue. From the structural point of view, natural bone is a highly complex and oriented hierarchical structure combination. From the perspective of macroscopic structure, it is composed of cancellous bone and compact bone; from the perspective of microstructure, it is composed of Harvard's system, bone unit, and trabecular bone; from the perspective ...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/22A61L27/18A61L27/50A61L27/56D04H1/728
CPCA61L27/025A61L27/222A61L27/18A61L27/50A61L27/56D04H1/728A61L2430/02A61L2300/412A61L2400/12C08L67/04
Inventor 莫秀梅张文彩刘明月郭刚潘信陈维明张小燕
Owner 诺一迈尔(苏州)生命科技有限公司
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