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Method for preparing denitrification carbon source from coffee pectin

A denitrification carbon source and pectin technology, applied in chemical instruments and methods, anaerobic digestion treatment, water pollutants, etc., can solve the problems of anaerobic treatment sludge domestication, microbial inactivation, and low treatment efficiency. Achieve good application prospects, reduce high influent concentration, and solve the effects of carbon source shortage

Pending Publication Date: 2022-08-09
GUILIN UNIVERSITY OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the amount of alcohol used in the ethanol precipitation method is too large, and the cost is high; the subsequent decolorization process of the flocculant treatment method is cumbersome; the biological contact oxidation treatment method is used, and the pectin is easy to adhere to the carrier of the biological contact oxidation tank, resulting in the absorption of microorganisms in the system. Insufficient oxygen leads to loss of activity, resulting in low treatment efficiency; there is a problem of sludge domestication in anaerobic treatment

Method used

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  • Method for preparing denitrification carbon source from coffee pectin
  • Method for preparing denitrification carbon source from coffee pectin
  • Method for preparing denitrification carbon source from coffee pectin

Examples

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Embodiment 1

[0029] A method for preparing denitrifying carbon source using coffee pectin, comprising the following steps:

[0030] (1) the coffee waste water containing pectin is flocculated and precipitated to obtain coffee pectin concentrate;

[0031] (2) Dissolve the prepared coffee pectin concentrate in a buffer solution and shake up, the buffer solution is acetic acid-sodium acetate, the reaction substrate concentration is 1:50, and the control pH is 4.5 to obtain a coffee pectin solution;

[0032] (3) Add 500U / g pectinase to the coffee pectin solution and shake well, and perform the hydrolysis reaction in a 40°C constant temperature water bath heating pot. The reaction time is 6h. After the reaction is completed, place it in a boiling water bath for 10min to stop the enzymatic activity;

[0033] (4) The inactivated hydrolyzate is put into a centrifuge, centrifuged at 5000 r / min for 10 min, and after the centrifugation is completed, the obtained supernatant is used as a denitrifying ...

Embodiment 2

[0035] A method for preparing denitrifying carbon source using coffee pectin, comprising the following steps:

[0036] (1) The coffee pectin concentrate is placed in a freeze dryer, freeze-dried for 48h, and then ground to make 100-mesh coffee pectin powder;

[0037] (2) Dissolve the coffee pectin powder in a buffer solution and shake up, the buffer solution is citric acid-sodium citrate, the reaction substrate concentration is 1:150, and the pH is controlled to be 4.5;

[0038] (3) Add 600U / g of pectinase to the coffee pectin solution and shake well, and perform the hydrolysis reaction in a 45°C water bath heating pot. The reaction time is 8h. After the reaction is completed, place it in a boiling water bath for 10min to stop the enzymatic activity;

[0039] (4) The inactivated hydrolyzate is put into a centrifuge, centrifuged at 5000 r / min for 10 min, and after the centrifugation is completed, the obtained supernatant is used as a denitrifying carbon source.

Embodiment 3

[0041] This embodiment is consistent with the other process parameters of Example 1, the difference is: in step (2), the prepared coffee pectin concentrate is dissolved in a buffer solution and shaken up, and the buffer solution is citric acid-sodium citrate, and the reaction The substrate concentration was 1:150, and the pH was controlled to be 4 to obtain a coffee pectin solution.

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Abstract

The invention discloses a method for preparing a denitrification carbon source by adopting coffee pectin, which comprises the following steps: (1) flocculating and precipitating coffee wastewater containing pectin to obtain a coffee pectin concentrated solution or freeze-drying the coffee pectin concentrated solution, taking out and grinding to prepare coffee pectin powder; (2) dissolving the coffee pectin concentrated solution or coffee pectin powder in a buffer solution, and controlling the pH value of the solution to obtain a coffee pectin solution; (3) adding pectinase, uniformly shaking, carrying out a constant-temperature hydrolysis reaction, and putting into a boiling water bath to terminate enzyme activity; and (4) performing centrifugal separation on the coffee pectin hydrolysate to obtain supernate, namely the denitrification carbon source. The method is simple in process and short in biological enzyme hydrolysis conversion time, resource utilization of coffee pectin can be achieved, and the problems of high inlet water concentration, large investment and high operation cost in coffee wastewater treatment are solved; the target of carbon circulation can be achieved, the operation cost of a denitrification process section is reduced, and good environmental benefits and economic benefits are achieved.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a method for preparing a denitrifying carbon source by using coffee pectin. Background technique [0002] The rapid development of my country's cities and economy has produced a large amount of municipal sewage. The N and P in the sewage will cause eutrophication of the water body, which needs to be treated before the sewage is discharged, and then discharged after reaching the standard. There are many methods for denitrification and dephosphorization of sewage, among which biological denitrification and dephosphorization have a wide range of applications in the field of sewage treatment. Biological nitrogen and phosphorus removal requires sufficient carbon sources as electron donors to ensure the metabolism of denitrifying bacteria and phosphorus accumulating bacteria. At present, the organic carbon concentration of municipal sewage is relatively low, and various comme...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F101/16C02F101/10
CPCC02F3/28C02F2101/16C02F2101/105Y02W10/10
Inventor 刘晨罗雪静李金城徐芝芬韦春满王潇潇时慧慧
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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