Paenibacillus phyllosphaeroides with raw starch hydrolysis capacity and application of paenibacillus phyllosphaeroides
A technology of bacillus and raw starch, applied in the biological field, can solve the problems of high energy consumption, high sugar production cost and high fermentation cost, and achieve the effects of low cost, reduced energy consumption, and reduced production cost.
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Embodiment 1
[0030] S1. Paenibacillus phyllodes Paenibacillus phyllosphaerae HYNU-YU148 was inoculated on the solid plate medium. The components and mass contents of the solid plate medium were: peptone 11.5g / L, yeast extract powder 4g / L, sodium chloride 6g / L, soluble starch 8g / L, agar 17.5g / L. The solid plate medium was placed in a 32°C constant temperature incubator for 2 days, and a single colony was picked from the solid plate medium with an inoculation loop into the liquid seed medium. The components and mass content of the liquid seed medium were: peptone 8g / L, yeast extract powder 4g / L, sodium chloride 4g / L, soluble starch 8g / L. 28°C, shaker cultured at 110rpm for 1 day as seed solution for use;
[0031] S2. The seed liquid cultivated in S1 is 1% by volume and is inserted into the raw starch fermentation medium for fermentation, and the raw starch fermentation medium components and mass contents are: raw starch 10g / L, ammonium sulfate 1g / L, Peptone 2g / L, dipotassium hydrogen phos...
Embodiment 2
[0035] S1. Paenibacillus phyllodes Paenibacillus phyllosphaerae HYNU-YU148 was inoculated on the solid plate medium. The components and mass content of the solid plate medium were: peptone g7.5g / L, yeast extract 6g / L, sodium chloride 6g4g / L, soluble starch 12g / L, agar 21.52g / L. The solid plate was placed in a constant temperature incubator at 2832 °C for 4 days, and a single colony was picked from the solid plate medium with an inoculation loop into the liquid seed medium. The components and mass content of the liquid seed medium were: peptone 12g / L, yeast extract powder 6g / L, sodium chloride 6g / L, soluble starch 11.52g / L. 32 ℃, 200 rpm shaker culture for 3 days as a seed solution for use;
[0036] S2. The seed liquid cultivated in S1 is 10% by volume and is inserted into the raw starch fermentation medium for fermentation, and the raw starch fermentation medium components and mass contents are: raw starch 100g / L, ammonium sulfate 3g / L , peptone 10g / L, dipotassium hydrogen...
Embodiment 3
[0040] S1. Paenibacillus phyllodes Paenibacillus phyllosphaerae HYNU-YU148 was inoculated on the solid plate medium. The components and mass contents of the solid plate medium were: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 5g / L, soluble starch 10g / L, agar 20g / L L. The solid plate was placed in a 30°C constant temperature incubator for 3 days, and a single colony was picked from the solid plate medium with an inoculation loop into the liquid seed medium. The components and mass content of the liquid seed medium were: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 5g / L, soluble starch 10g / L. 30 ℃, 150 rpm shaker culture for 2 days as a seed solution;
[0041] S2. the seed liquid cultivated in S1 is 5% by volume and is fermented in the access medium, and the fermentation medium components and mass contents are: raw starch 50g / L, ammonium sulfate 2g / L, peptone 10g / L, Dipotassium hydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.5g / L, calcium...
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