Process for preparing gelatin from enzyme degradation bone collagen

Abstract

The invention belongs to the field of gelatin preparation, in particular to a method for enzymatically degrading bone collagen to prepare gelatin. (1) Use degreased and non-demineralized aggregates to mix with water and grind them into bone slurry, and the bone particle size is controlled at 1-5mm; (2) Adjust the pH value to 1.5-4 with acid, or adjust the pH value to 7-8 with alkali, Add acid or partial alkaline protease to control collagen degradation; the amount of protease added is 2-8‰ of the bone mud weight; (3) react at room temperature for 7-10 hours, adjust the pH of the solution to 5.0-6.5 with alkali or acid , heating the temperature to 70 to 85°C for extraction; (4) separating the turbid gelatin solution obtained from the extraction; (5) filtering the glue solution to obtain a high-purity hard capsule gelatin solution. The dried gelatin is tested, and the molecular weight distribution of gelatin is α=48%, β=18%; the jelly strength is over 240 grams; the viscosity is 3.0mPs; the transmittance is 90% at 620nm and 71% at 450nm.

Description

technical field [0001] The invention belongs to the field of gelatin preparation, and in particular relates to a method for preparing gelatin by enzymatically degrading bone collagen to produce high-purity, high-grade gelatin suitable for producing hard capsules. Background technique [0002] Gelatin is a protein extracted from the connective tissue (skin and bone) of animals (cattle, horse, pig) through a multi-step long degradation. The gelatin industry has a history of more than 100 years, and has been extracting bone gelatin from bone collagen by using the traditional alkaline process. The biggest advantage of the traditional alkaline process to prepare gelatin is: simple operation, separate product quality grades, the highest quality photographic glue can be obtained, and a certain proportion of industrial glue can also be produced. However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkalin...

AI Technical Summary

Problems solved by technology

However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkaline process: first, the production cycle is long, ranging from 60 to 100 days depending on the season, climate and region

The great disadvantage brought by the long production cycle is that the production efficiency is extremely low, especially the quality of gelatin produced between different batches cannot be guaranteed to be unchanged

Second, the water consumption is large, 1 / 2 of the 18 processes require water, especially the three major steps of washing, pickling and liming require a lot of water

Third, the pollution of the environment is serious, and a large amount of waste acid and waste liming water are discharged nearby, causing serious environmental pollution in surrounding areas

A large number of literature surveys show that, so far, these developed countries have not been able to produce pharmaceutical gelatin with new technology.