Method for extracting diosgenin for joint production of starch suger by prehy drolysis of yellow ginger by multienzyme process

A starch sugar, pre-hydrolysis technology, applied in steroids, fermentation, organic chemistry, etc., can solve the problems of large amount of waste water, high consumption of raw materials, low alcohol concentration, etc., to reduce chemical oxygen consumption, reduce acid hydrolysis The effect of improving the total amount and saponin yield

Inactive Publication Date: 2006-05-10
TAIGU INVESTMENT WUHAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fresh turmeric contains starch ≥ 18%, and dried turmeric contains starch ≥ 45%, which is discharged from waste water after acid hydrolysis, resulting in waste of resources
[0004] 2. The composition of wastewater is complex, the discharge volume is large, and the pollution is serious
[0005] 3. The yield of saponin is low, and the consumption of raw materials is large. The yield of fresh turmeric saponin is 0.6-0.8%, and the yield of dry turmeric saponin is 1.8-2.0%.
This method has following shortcoming: 1, in order to guarantee saponin yield when separating cellulose and starch, turmeric is repeatedly refined and washed, resulting in low starch concentration; 2, because turmeric contains many soluble starches and small particle size, starch and The separation of water is difficult, the alcohol concentration produced is low, and the energy consumption of refining and alcohol distillation is large; 3. When separating saponin-containing lees from mash, filtration is very difficult due to the existence of yeast; 4
The process is complex, the investment is large, and the amount of wastewater produced is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fresh turmeric 1000g, soak in water at normal temperature for 6 hours, wash it, and pulverize it with a universal pulverizer to obtain a slurry with a particle size of about 1mm, mix it with water according to the ratio of fresh turmeric to water 1:2.5; place the slurry in Rapidly heat up to 90-95°C in a 5000ml beaker, add 2.0ml (enzyme solution concentration 18000U / L, the same below) commercially available α high-temperature amylase, and enzymolyze at 90-95°C for 2.5 hours under stirring; wait until the temperature drops to 55 ℃, add 3.5ml (enzyme solution concentration 18000U / L the same below) commercially available glucoamylase, react at 55-60℃ and pH4-5 for 8 hours to obtain saccharified material; saccharified material is centrifuged to obtain filter cake and sugar solution, the saccharified solution is filtered with a hollow fiber UF membrane at 40-50°C; the centrifugal filter cake and the membrane filter residue are combined, and a hydrochloric acid solution with a...

Embodiment 2

[0020] Soak 400g of dried turmeric slices (moisture content 10%) in water at room temperature for 10 hours, wash it, and pulverize it with a universal grinder to obtain a powder with a particle diameter of about 4mm. The powder is 1:6.5 according to the ratio of turmeric to water Mix and refine to obtain a slurry with a particle size of 0.5-1mm; place the slurry in a 5000ml beaker and rapidly heat up to 85°C, add 3ml of commercially available α-medium-temperature amylase (the same as the enzyme solution concentration of 18000U / L), and stir Enzymolysis at 80-90°C for 2 hours; when the temperature dropped to 55°C, add 4.5ml of commercially available glucoamylase, react at 55-60°C and pH4-5 for 10 hours to obtain a saccharification solution; the saccharification solution was separated by centrifugation , its filtrate is 100nm zirconia membrane filtration with aperture at 40-50 DEG C; Combine centrifugal filter cake and membrane filter residue, add the hydrochloric acid solution of...

Embodiment 3

[0022] 1000g of fresh turmeric, soaked in water at normal temperature for 8 hours, washed it, and pulverized it with a universal pulverizer to obtain a powder with a particle size of 2-3mm. The powder was mixed and refined according to the ratio of turmeric and water in a ratio of 1:2. Obtain a slurry with a particle size of 0.5-1mm; place the slurry in a 5000ml beaker and rapidly heat up to 95°C, add 4ml of commercially available α-high temperature amylase, and enzymolyze it at 95-100°C for 3 hours under stirring; wait until the temperature drops to At 60°C, add 2.0ml of commercially available glucoamylase, and react at 55-60°C and pH4-5 for 6 hours to obtain a saccharified liquid; the saccharified liquid is separated by centrifugation, and the filtrate is oxidized at 40-50°C with a pore size of 200nm Aluminum membrane filtration; Merge centrifugal filter cake and membrane filter residue, add water 500ml to wash filter residue, add the hydrochloric acid solution of concentrati...

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Abstract

The invention provides a saponin extraction method for prehydrolyzing turmeric and co-producing starch sugar by a multi-enzyme method. The method is to first wash the turmeric, grind it into a slurry, then add α-amylase to liquefy, then add glucoamylase to saccharify, then heat up and inactivate, and then the saccharified material is centrifuged to obtain filter cake and starch sugar solution. The ultrafiltration membrane with a molecular weight cut-off of 1000-10000daltons or the microfiltration membrane of 100-200nm is separated to obtain starch sugar solution, and the filter residue is mixed into the centrifugal filter cake to obtain sugar residue, which is hydrolyzed by adding hydrochloric acid or sulfuric acid, and the hydrolyzate is filtered, washed, Drying, solvent extraction to get saponin products. Before acid hydrolysis, the present invention adopts multi-enzyme starch hydrolysis sugar production and membrane separation technology, reduces the total amount of acid hydrolyzate, saponin hydrolysis is more complete, saponin yield is high, waste water volume is small, and chemical oxygen consumption is low. This method can also be used for the extraction of diosgenin from the co-production of starch sugar by the hydrolysis of pendirol.

Description

technical field [0001] The invention relates to a method for extracting saponin (sapogenin) by using Dioscorea scutellariae and turmeric to prehydrolyze and co-produce starch sugar through a multi-enzyme method. Background technique [0002] At present, most of the methods for domestic production of saponin are to adopt the process of direct acid hydrolysis of turmeric to prepare saponin after crushing and pre-fermentation. This method mainly has the following problems: [0003] 1. The starch component of turmeric has not been utilized. Fresh turmeric contains starch ≥ 18%, dried turmeric contains starch ≥ 45%, and it is discharged from waste water after acid hydrolysis, resulting in waste of resources. [0004] 2. The composition of wastewater is complex, the discharge volume is large, and the pollution is serious. Wastewater discharged per ton of saponin 450-500M 3 , where COD value is 30000~110000mg / L; pH value: 0.2-1.5. [0005] 3. The yield of saponin is low, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12P19/14C07J71/00
Inventor 李华才徐晓东郭永龙张金安
Owner TAIGU INVESTMENT WUHAN
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