Skin care composition
A technology of skin care, composition, applied in the field of topical composition, which can solve the problem of ungeneralized application, synergistic combination form of conjugated linoleic acid, etc., to achieve improved skin tone, broad skin care benefits, texture and condition Improved effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0044] This example illustrates the synthesis of coupled linoleic acid containing the c9 t11 isomer and the t10 c12 isomer. Preparation of Mixed Isomers of CLA
[0045] 'Analar reagent' (AR) sodium hydroxide (0.6 kg) was dissolved in 6 kg of pharmaceutical grade propylene glycol by mixing and heating at 80-85°C. The sample was allowed to cool and 2 kg of safflower oil was added. The mixture was refluxed at 170°C for 3 hours with rapid stirring using standard small-scale equipment. The reaction mixture was cooled to about 95°C, the stirrer was reduced to medium speed, and the mixture was neutralized with 1.280 L of 35.5% hydrochloric acid dissolved in demineralized water (8 L), maintaining the temperature at about 90°C. The reaction mixture was allowed to stand and the aqueous phase was removed. The oily phase was washed with 2 x 1 L of 5% AR salt emulsion and 2 x 1 L of demineralized water at 90 °C, discarding any soapy material. The CLA-enriched oil was dried under vacuum...
Embodiment 4
[0057] Phase 2 (P2) primary human foreskin fibroblasts were seeded in 12-well plates at 10,000 cells / cm 2 and maintained for 24 hours in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum in an atmosphere of 5% carbon dioxide and 4% oxygen. Thereafter, cells were washed with serum-free DMEM and incubated in fresh serum-free DMEM for 60 minutes. The fibroblast monolayer was then washed again with serum-free DMEM. Assay reagents and vehicle controls were added to cells in triplicate to a final volume of 0.4 ml / well fresh serum-free DMEM and further incubated for 24 hours. This fibroblast conditioned medium was either assayed immediately or snap frozen in liquid nitrogen and stored at -70°C for further analysis. Thereafter cells were counted and data from dot blot analysis were normalized to cell number. Example 4 Dot blot analysis of procollagen-I and decorin protein in skin fibroblast conditioned medium
[0058] Samples of conditioned medium fr...
Embodiment 5
[0061] Table 5 below shows the synergistic effect of coupled linoleic acid in combination with the phenolic compound epigallocatechin gallate (EGCG) on procollagen-I and / or decorin synthesis in human skin fibroblasts, Also the amount of active agent applied is given. In order to normalize the results, the effect of the test substances was determined relative to the vehicle-treated control value of 100 random units. The concentration of the reagents used in the assay had no effect on the viability of the cells.
[0062] deal with
[0063] The results in Table 5 show that the combined form of coupled linoleic acid and epigallocatechin gallate synergistically up-regulates the expression of procollagen-I and / or decorin in human skin fibroblasts compared to the control. synthesis. Levels of decorin in the skin are associated with improvements in skin condition and appearance. Increasing decorin levels in the skin is important for controlling and correcting collagen de...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap