Batch production process of biological herbicide

A herbicide and large-scale technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of vitality decline, complicated process, delay in commercialization process, etc., and achieve the effect of uniform growth

Active Publication Date: 2004-04-07
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the herbicidal active unit of biological herbicides is often the active part of fungi, such as asexual propagules such as mycelia, conidia, and chlamydospores, and fungal active parts such as conidia produced by liquid fermentation are in the fermentation broth. Preservation, vitality declines faster
For example the biological herbicide Devine  It was the first to be developed as a biological herbicide, and its infestation unit is a fresh suspension of chlamydospo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 The herbicidal active ingredient of G. anthracnose G. anthracis G. anthracis Popona specialized strain QZ-97a (patent application number is 00112506.0) is the conidia of this fungus. The specific implementation method is as follows: the bacterial strain QZ-97a is cultured on PDA medium for 7 days, inoculated into corn flour, soybean flour and sucrose liquid medium and cultured for 5-7 days, and pulverized by a pulverizer to obtain a bacterial cell suspension. The solid medium is weighed from wheat bran, soybean powder and sweet potato powder according to the dry weight ratio of 7:2:1, then add water 1.5 times the weight of the dry material, mix well and sterilize. Inoculate the bacterial suspension onto the sterilized solid medium in a sterile environment, and culture it aerobically in a self-made incubator for 3-7 days. The temperature of the room is controlled by an air conditioner at 20°C. The amount of spores produced per gram of dry matter Up to 7.8×10 9...

Embodiment 2

[0075] Example 2 Alternaria adenophorum uses crude metabolites to control weeds. The specific implementation method is as follows: the Alternaria strain (patent application number is 00112560.5) is cultivated on PDA medium for 7 days, and inoculated into soybean flour and corn flour Cultured in sucrose liquid medium for 7 days, crushed with a pulverizer to obtain mycelium suspension. The solid medium is weighed from the cornmeal bean cake according to the dry weight ratio of 1:1, then add water 0.33 times the weight of the dry material, mix well and sterilize. Inoculate the bacterium suspension onto the sterilized solid medium in a sterile environment, seal it with a fresh-keeping bag, punch holes appropriately, and culture at a temperature of 25°C for 10 days. After the cultivation, the crude toxin was prepared by air-drying, and after dilution, the biological activity was tested by acupuncture method, and the toxin-producing ability was expressed by measuring the diameter of...

Embodiment 3

[0110] Example 3 The herbicidal active ingredient of Bifucus spp. is the conidia of this fungus. The specific implementation method is as follows: bacterial strain QZ-2000 (patent application number is 011340020.9), cultured on PDA medium for 5 days, inoculated into corn flour soybean flour sucrose liquid medium and cultivated for 5-7 days, pulverized with pulverizer to obtain mycelia body suspension. The solid medium is made of soybean flour + cottonseed cake and weighed according to the dry weight ratio of 2:1, then add water 0.6 times the weight of the dry material, mix well and sterilize. Inoculate the bacterium suspension onto the sterilized solid medium in a sterile environment, cultivate it aerobically in a self-made incubator for 3-8 days, place it under a 20W black light (wavelength 365nm), and irradiate it with a fluorescent lamp for 1-3 days. Day, or interval irradiation of ultraviolet light for a total of 1-10 hours, the temperature of the room is controlled at 28...

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Abstract

The present invention belongs to the field of application of microbe in agricultural plant protection. The biological herbicide is batch produced with fungus strain psorosperm anthrax bacteria QZ-97a, purplesteem eupatorium hypnocyst, crab grass hyperbolic spora, common zinnia hypnocyst, etc. and through combined liquid and solid fermentation. During the liquid and solid fermentation, the agricultural product leftover is used as culture medium material, and proper initial temperature is benefit for thallus to obtain nutritive matter and oxygen transmitted and to obtain high conversion rate. Solid fermentation has wider temperature range and easy-to-obtain lighting condition. The process is simple and easy to operate.

Description

1. Technical field [0001] The invention relates to a method for mass production of biological herbicides, which belongs to the technical field of the application of microorganisms to agricultural plant protection and control of crop weeds, and is specially used for the biological control of weeds in farmland. 2. Technical background [0002] At present, domestic weed control mainly depends on chemical pesticide products, and its usage and area are still expanding. With the integration of global economy and the internationalization of trade, the national pesticide industry is facing severe challenges. The high research and development costs of new varieties of chemical pesticides have hindered the development of the pesticide industry and the needs of agricultural production. On the other hand, the extensive use of chemical pesticides has caused and is causing serious environmental pollution problems, directly endangering the health of humans and animals and affecting the sus...

Claims

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Application Information

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IPC IPC(8): A01N63/00
Inventor 强胜朱秦朱云枝徐春凤安传福宋小玲戴宝江蔡建国
Owner NANJING AGRICULTURAL UNIVERSITY
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