Batch production process of biological herbicide
A herbicide and large-scale technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of vitality decline, complicated process, delay in commercialization process, etc., and achieve the effect of uniform growth
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Embodiment 1
[0026] Example 1 The herbicidal active ingredient of G. anthracnose G. anthracis G. anthracis Popona specialized strain QZ-97a (patent application number is 00112506.0) is the conidia of this fungus. The specific implementation method is as follows: the bacterial strain QZ-97a is cultured on PDA medium for 7 days, inoculated into corn flour, soybean flour and sucrose liquid medium and cultured for 5-7 days, and pulverized by a pulverizer to obtain a bacterial cell suspension. The solid medium is weighed from wheat bran, soybean powder and sweet potato powder according to the dry weight ratio of 7:2:1, then add water 1.5 times the weight of the dry material, mix well and sterilize. Inoculate the bacterial suspension onto the sterilized solid medium in a sterile environment, and culture it aerobically in a self-made incubator for 3-7 days. The temperature of the room is controlled by an air conditioner at 20°C. The amount of spores produced per gram of dry matter Up to 7.8×10 9...
Embodiment 2
[0075] Example 2 Alternaria adenophorum uses crude metabolites to control weeds. The specific implementation method is as follows: the Alternaria strain (patent application number is 00112560.5) is cultivated on PDA medium for 7 days, and inoculated into soybean flour and corn flour Cultured in sucrose liquid medium for 7 days, crushed with a pulverizer to obtain mycelium suspension. The solid medium is weighed from the cornmeal bean cake according to the dry weight ratio of 1:1, then add water 0.33 times the weight of the dry material, mix well and sterilize. Inoculate the bacterium suspension onto the sterilized solid medium in a sterile environment, seal it with a fresh-keeping bag, punch holes appropriately, and culture at a temperature of 25°C for 10 days. After the cultivation, the crude toxin was prepared by air-drying, and after dilution, the biological activity was tested by acupuncture method, and the toxin-producing ability was expressed by measuring the diameter of...
Embodiment 3
[0110] Example 3 The herbicidal active ingredient of Bifucus spp. is the conidia of this fungus. The specific implementation method is as follows: bacterial strain QZ-2000 (patent application number is 011340020.9), cultured on PDA medium for 5 days, inoculated into corn flour soybean flour sucrose liquid medium and cultivated for 5-7 days, pulverized with pulverizer to obtain mycelia body suspension. The solid medium is made of soybean flour + cottonseed cake and weighed according to the dry weight ratio of 2:1, then add water 0.6 times the weight of the dry material, mix well and sterilize. Inoculate the bacterium suspension onto the sterilized solid medium in a sterile environment, cultivate it aerobically in a self-made incubator for 3-8 days, place it under a 20W black light (wavelength 365nm), and irradiate it with a fluorescent lamp for 1-3 days. Day, or interval irradiation of ultraviolet light for a total of 1-10 hours, the temperature of the room is controlled at 28...
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