Domestic animal schistosome antibody gold-labeled immunization fast detection kit and method for preparation

Abstract

The invention relates to an agent box and method for measuring the livestock bilharziasis antibody, which uses the soluble Japan schistosome ovum antigen as sample antigen, and uses the colloidal gold to label Staphylococcus aureus A protein. Said invention combines the diagnosis with the technique which uses colloidal gold to label protein and sets up the spot electrosyneresis method, and the agent box based on this technique has high sensitivity and the result can be discriminated with naked eye. The test has proved that the method measuring the Schistosoma bovis has the sensitivity of 93.3 %, specificity of 98.0%, the index is 0.913.

Description

technical field [0001] The invention relates to a kit for rapid immunodiagnosis of parasitic diseases, in particular to a kit and a preparation method for detecting domestic animal schistosome antibody by using colloidal gold-labeled Staphylococcus aureus protein A with soluble Schistosoma japonicum egg antigen as spotting antigen. Background technique [0002] Schistosomiasis is the main parasitic disease in developing countries. According to the World Health Organization (WHO) estimate in 1993, there are 74 countries and regions with a population of 3.1 billion in the world. The population is 600 million, of which 200 million people are affected Infection, the number of people who die from this disease reaches as many as one million people every year. In my country, schistosomiasis is mainly prevalent in the Yangtze River Basin and 12 provinces, autonomous regions, and municipalities south of it. Most of the endemic areas are important agricultural production bases with de...

AI Technical Summary

Problems solved by technology

Existing methods for domestic animal schistosomiasis are mainly etiological investigation and serological investigation. The etiological method mainly adopts feces miracidium hatching method, which has high specificity and can be used to diagnose cattle, sheep and other livestock. The operation is time-consuming and labor-intensive, the sensitivity is low, and the missed detection rate is high (up to 81.8%), so it is not suitable for livestock diagnosis in low-endemic areas

Serological methods have high sensitivity and specificity, which can reduce missed detection and make up for the deficiencies of stool examination, including ring egg precipitation test (COPT), indirect hemagglutination test (IHA), latex agglutination test (COPS), enzyme Linked immunosorbent assay (ELISA), monoclonal antibody dot enzyme-linked immunosorbent assay, etc., but the above-mentioned methods are cumbersome to operate, time-consuming, and some require certain instruments, so that they can be used in rapid clinical diagnosis, rapid animal quarantine and schistosomiasis. The application of screening is limited, and it is difficult to promote to grassroots units