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Method for producing bacterial xylanase

A technology of xylanase and production method, which is applied in the directions of biochemical equipment and methods, bacteria, enzymes, etc., can solve the problems of increased variation of fermentation parameters, unstable product quality, and many safety problems, and achieves a wide range of active pH values. Scope, easy control of production process and stable product quality

Inactive Publication Date: 2005-10-05
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It uses fungi and solid fermentation technology to produce xylanase. The final product is a multi-enzyme complex. The variation of fermentation parameters is large, it is difficult to control, the product quality is unstable, the amount of addition is large, and the production cost is high.
In particular, the resistance to heat treatment is low, the loss of enzyme activity is large during the feed pelleting process, the storage period is short, and there are many safety problems in practical application.

Method used

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  • Method for producing bacterial xylanase

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Experimental program
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Effect test

Embodiment 1

[0025] 1. Cryopreservation of strains: prepare suspension of Bacillus subtilis, freeze-dry, store the frozen bacteria in a nitrogen-filled sealed ampoule, and store them in a -80°C low-temperature refrigerator with a shelf life of 8-10 years .

[0026] 2. Rejuvenation and screening of frozen strains: Dissolve the bacterial powder in sterile physiological saline, inoculate it on the rejuvenation medium, and cultivate at 37°C for 48 hours to screen single bacterial cells with larger colonies, smooth and moist surfaces . Among them, the slant medium is: beef extract 3g, peptone 10g, sodium chloride (NaCl) 5g, agar 20g, add water to make up to 1000ml, pH 7.0-7.2, sterilize at 121°C for 20min, and culture the sterilized slant. The base was placed in a greenhouse at 37°C for 24-48h to check whether the sterilization was complete.

[0027] 3. Mother bottle culture: The activated and expanded seeds are cultured in a shaker flask, 50ml of culture solution is placed in a 500ml shaker ...

Embodiment 2

[0034] 1. Activation and expansion of strains: Dissolve the frozen preserved Bacillus subtilis cell powder in sterile physiological saline, inoculate it on the rejuvenation medium, and cultivate at 33 °C for 52 hours, and screen for single bacteria with larger colonies, smooth and moist surfaces body. Among them, the slant medium is: beef extract 2g, peptone 8g, sodium chloride (NaCl) 4g, agar 18g, add water to make up to 1000ml, pH 7.0-7.2, sterilize at 121°C for 20min, and culture the sterilized slant. The base was placed in a greenhouse at 37°C for 24-48h to check whether the sterilization was complete.

[0035] 2. Bottle culture: the activated and expanded seeds are cultured in a shaker flask, 50ml of culture medium is placed in a 500ml shaker flask (triangular flask), the rotation speed is 180rpm, the culture temperature is 37°C, and the time is 24h; the shaker flask culture medium is: beef Paste 2g, peptone 8g, NaCl 4g, add water to make up to 1000ml, pH7.0-7.2, sterili...

Embodiment 3

[0042] 1. Activation and expansion of strains: Dissolve the frozen preserved Bacillus subtilis cell powder in sterile physiological saline, inoculate it on the rejuvenation medium, and cultivate at 39°C for 40 hours to screen single bacteria with larger colonies, smooth and moist surfaces body. Among them, the slant medium is: beef extract 5g, peptone 12g, sodium chloride (NaCl) 6g, agar 22g, add water to make up to 1000ml, pH 7.0-7.2, sterilize at 121°C for 20min, and culture the sterilized slant. The base was placed in a greenhouse at 37°C for 24-48h to check whether the sterilization was complete.

[0043] 2. Bottle culture: The activated and expanded seeds are cultured in a shaker flask, 50ml of culture medium is placed in a 500ml shaker flask (triangular flask), the rotation speed is 200rpm, the culture temperature is 39°C, and the time is 24h; the shaker flask culture medium is: beef Paste 5g, peptone 12g, NaCl 6g, add water to make up to 1000ml, pH7.0-7.2, sterilize at...

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Abstract

This invention is a manufacturing method of bacterial xylanase. Its characteristics are: adopt heat-fast subtilin as creating bacterial seed and manufacture bacterial xylanase through liquid deep-seated fermentation. The bacterial xylan ase of this method has high hydrolysis and can effectively hydrolyze the substance to produce the alkali ase with low or high molecular weights, it has wide range of pH value and has better function in different animal livers. This invention adopts deep-seated liquid fermentation to produce bacterial xylanase with good enzyme performance, and the producing glow is easily controlled, the manufacturing cycle is short, the quality of products is stable, and it is suitable for industrial manufacture and the roboticized degree is high.

Description

technical field [0001] The present invention relates to a production process of xylanase, in particular to a production process of bacterial xylanase. Background technique [0002] Arabinoxylan (AX) is a non-starch polysaccharides (NSP) widely present in common plant feed materials (maize, wheat, rapeseed and cottonseed) in China. the main anti-nutritional factor. Therefore, eliminating the anti-nutritional effect of arabinoxylan is of great significance to further improve the utilization rate of feed resources, expand feed resources and improve the economic benefits of breeding. A large number of studies at home and abroad have shown that adding exogenous xylanase and its compound enzyme preparations to feed can improve the utilization rate of feed nutrients and improve animal production performance. [0003] Studies on the enzymatic mode of action of xylanase showed that endo-xylanase can randomly attack the 1-4-β glycosidic bond of xylan and preferentially attack non-su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/24C12N9/42
Inventor 詹志春
Owner WUHAN SUNHY BIOLOGICAL
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