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Method for extracting volatile oil and liposoluble alkaloid from plumula nelumbini

A technology of biological total alkaloids and lotus seed heart, which is applied in the direction of alkaloids, alkaloids, organic chemical methods, etc., can solve the problems of low efficiency of removing impurities such as chlorophyll, waste of resources, large consumption of toxic solvent methanol, etc., and achieve alkaloid production And the content of active ingredients is high, the effect is good

Inactive Publication Date: 2006-01-25
TIANJIN UNIV
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  • Summary
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Problems solved by technology

The advantage of this method is that the process is simple, but the extraction time is long, the consumption of toxic solvent methanol is large, the yield of fat-soluble total alkali is low, and the efficiency of removing impurities such as chlorophyll is low
In addition, during the purification and separation process involved in the above two alkaloid extraction methods, a large number of other active ingredients in the lotus seed heart, such as flavonoids, unsaturated fatty acids, sitosterol and vitamin E, are also used as impurities along with the purification process. removed, which largely resulted in a waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Put 350 g of crushed Jiangxi-produced lotus seed core raw materials into the extraction kettle, the extraction pressure is 20 MPa, and the temperature is 30 °C; the primary separation temperature is 30 °C, and the pressure is 10 MPa; the secondary separation temperature is 20 °C, and the pressure is 4 MPa. The supercritical fluid was statically soaked in the raw material for 1 hour, and after the equipment stabilized, the dynamic extraction was carried out, and the product was collected by the receiver. Finally, 24.6g of the volatile oil of the lotus seed heart was obtained, and the yield of the volatile oil of the lotus seed heart was 7.03%.

[0012] Gas chromatography-mass spectrometry (GC-MS) analysis method; HP6890 / 5973N GC / MS type gas chromatography-mass spectrometry (USA); HP-5 elastic quartz capillary column (50m×250μm×0.25μm); injection volume 1μl ; carrier gas He; column front pressure 50kPa; split ratio 100:1; GC-MS interface temperature 280°C. Temperature pro...

example 2

[0016] Put 350 g of crushed Jiangxi-produced lotus seed core raw materials into the extraction kettle, the extraction pressure is 40 MPa, and the temperature is 50 ° C; the primary separation temperature is 40 ° C, and the pressure is 15 MPa; the secondary separation temperature is 30 ° C, and the pressure is 6 MPa. Regulate supercritical equipment parameter, finally get 38.0g of lotus seed heart volatile oil, and the volatile oil yield of lotus seed heart is 11.6%. The GC-MS separation and identification results were basically the same as Example 1.

[0017] Add 2500ml of 90% ethanol to the lotus seed heart extract residue after supercritical extraction for heat reflux extraction, heat reflux extraction 3 times, each time for 2 hours, combine the extracts, filter through gauze, and evaporate the ethanol under reduced pressure to obtain a concentrated extract. Dilute hydrochloric acid to adjust the pH to about 1.0, filter through a 300-mesh sieve, stir and heat the filtrate to...

example 3

[0018] Put 350 g of crushed Jiangxi-produced lotus seed core raw materials into the extraction kettle, the extraction pressure is 30 MPa, and the temperature is 65 °C; the primary separation temperature is 50 °C, and the pressure is 15 MPa; the secondary separation temperature is 40 °C, and the pressure is 6 MPa. Regulate supercritical equipment parameter, finally get 31.2g of lotus seed heart volatile oil, and the volatile oil yield of lotus seed heart is 8.91%. The GC-MS separation and identification results were basically the same as Example 1.

[0019] Add 3000ml of 80% ethanol to the lotus seed extract residue after supercritical extraction for heat reflux extraction, heat reflux extraction twice, each time for 3 hours, combine the extracts, filter through gauze, and evaporate the ethanol under reduced pressure to obtain a concentrated extract. Dilute hydrochloric acid to adjust the pH to about 2.0, filter through a 250-mesh sieve, stir and heat the filtrate to 90°C, add ...

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Abstract

A process for extracting volatile oil and liposoluble alkaloid from the core of lotus seed includes such steps as pulverizing, supercritical CO2 extraction of volatile oil at 30-60 deg. C under 20-40 MPa, mixing the dregs with alcohol, thermal reflux extracting, vacuum evaporation to recover solvent and obtain the alkaloid extract, acidifying, filter, heating while stirring and adding absorbent cotton to remove impurities including chlorophyll, adding organic solvent, and alkalizing to obtain said liposoluble alkaloid.

Description

technical field [0001] The invention relates to a method for extracting volatile oil and fat-soluble total alkaloids from lotus seeds, which belongs to the technology of separating and extracting medicinal active ingredients from lotus seeds. Background technique [0002] Lotus seed heart is the green germ and radicle of the mature seeds of Nelumbo nucifera Gaertn., a plant of the water lily family. It has the effects of clearing heart, removing heat, stopping bleeding, and astringent essence. The medicinal active ingredients of lotus seed heart are mainly alkaloids, including fat-soluble alkaloids such as liensinine, isoliensinine and methyl-liensinine, as well as nuciferine, pro-nuciferine, liensinine quaternary ammonium, and methyl liensinine. Water-soluble alkaloids, such as demethyl higinarine, among which fat-soluble alkaloids have obvious pharmacological effects of relaxing and lowering blood pressure. Recent studies have shown that the volatile oil in the lotus seed...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P7/04A61P9/12C07B63/00C07G5/00
CPCY02P20/54
Inventor 李淑芬肖鸾尚宇光杨静唐韶坤田松江
Owner TIANJIN UNIV
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