An enzyme linked immuno-detection kit suitable for furazolidone retention analysis and application

An enzyme-linked immunosorbent assay and furazolidone technology, applied in the field of immunochemical analysis, can solve problems such as no patent reports on furazolidone ELISA kits
CN1731185AInactive Publication Date: 2006-02-08HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2006-02-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an enzyme immune detecting agent box suit for analyzing the surplus furazolidone and its application in the field of chemo immunology analyzing technology. The agent box is mainly formed by a 3-amido-2-AOZ peculiarity antibody, a AOZ standard solution which comprises AOZ and the ferment standard plate of the egg albumin complex. The sample releases AOZ after alcaine hydrolysis, then the benzaldehyde derivatizes over the night, MAX column purifies, it adopts indirect contest ELISA method to detect the animal structure such as liver and the AOZ surplus in muscle. The core technology comprises: artificial antibody synthesis, antibody preparing, ELISA method establishing, and ELISA agent box adjustment.
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Description

technical field

[0001] The invention relates to an enzyme-linked immunoassay kit suitable for the analysis of furazolidone residues, which is suitable for determining the residues of furazolidone residue marker 3-amino-2-oxazolidinone (abbreviated as AOZ, the same below) in animal foods. The invention belongs to the technical field of immunochemical analysis. Background technique

[0002] Furazolidone, also known as furazolidone, belongs to nitrofuran drugs and is a synthetic broad-spectrum antibacterial drug. It was once widely used as a growth-promoting agent in livestock, poultry, and aquaculture. Toxicology studies have found that furazolidone is carcinogenic and mutagenic. The European Union listed furazolidone as a banned drug in 1995, and then the United States, Japan, Australia and other countries all canceled the use of furazolidone in livestock and poultry production. The "List of Veterinary Drugs and Other Compounds Banned for Fo...

Claims

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