Double-template method hydroxyethyl cellulose modified alginate microsphere and its preparation method

A technology of hydroxyethyl cellulose and alginate, which is applied in material inspection products, biological testing and other directions, can solve the problems of poor compatibility, poor stability, and high amount of modified components, and achieve good compatibility and price. Easily available and inexpensive effects

Inactive Publication Date: 2006-07-26
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of modified components in the above-mentioned several modification methods is relatively large.
Sodium alginate / gelatin or sodium alginate / chitosan, sodium alginate / polylysine complex system is less stable in ionic solution environment
However, the modified component PVA used in the sodium alginate / polyvinyl alcohol (PVA) system has poor compatibility with sodium alginate.

Method used

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  • Double-template method hydroxyethyl cellulose modified alginate microsphere and its preparation method
  • Double-template method hydroxyethyl cellulose modified alginate microsphere and its preparation method
  • Double-template method hydroxyethyl cellulose modified alginate microsphere and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0039] Preparation of hydroxyethyl cellulose modified alginate microspheres (Alg / HEC) by emulsion template method:

[0040] (1) Dissolve 0.6g of sodium alginate in 20ml of deionized water to prepare a 3% sodium alginate solution, add 1ml of 2% HEC solution, mix well, add 1g of liquid paraffin, 0.1g of Span85, 0.4g of Tw80, and stir for 1h , to become a uniform milky white emulsion;

[0041] (2) Disperse the emulsion in the organic oil phase under constant mechanical stirring. The composition of the oil phase is: chloroform 16ml, n-hexane 24ml; surfactant: span80 0.8g, span85 0.8g;

[0042] (3) After stirring for 5 minutes, add 5 ml of 2% PVP aqueous solution (cosurfactant) to the suspension system, and continue stirring for 15 minutes;

[0043] (4) Add 20ml 3% CaCl 2 , stir for 30min;

[0044] (5) The microspheres were separated from the suspension, rinsed with a large amount of deionized water, and then crosslinked in 5% glutaraldehyde (pH=6.00) crosslinking solution for ...

Embodiment 2

[0057] Preparation of hydroxyethyl cellulose modified alginate microspheres (BSA-Ca-Alg / HEC) by macromolecule template method:

[0058] (1) Add 0.6 g of sodium alginate to 20 ml of 20 μmol / LBSA aqueous solution, after fully dissolving, add 1 ml of 2% HEC solution, and mix well;

[0059] (2) Disperse the mixed liquid in the organic oil phase under the condition of constant speed mechanical stirring. The composition of the oil phase is: chloroform 16ml, n-hexane 24ml; surfactant: span80 0.8g, span85 0.8g;

[0060](3) After stirring for 5 minutes, add 5 ml of 3% PVP aqueous solution (cosurfactant) to the suspension system, and continue stirring for 15 minutes;

[0061] (4) Add 20ml 3% CaCl 2 , stir for 30min;

[0062] (5) The microspheres were separated from the suspension, rinsed with a large amount of deionized water, and then crosslinked in 5% glutaraldehyde (pH=6.00) crosslinking solution for 48 hours;

[0063] (6) The fully cross-linked microspheres were taken out of the...

Embodiment 3

[0082] Fully mix 20ml of 3% sodium alginate solution with 1.5ml of 2% hydroxyethyl cellulose, add 0.8g of liquid paraffin, 0.16g of Span85, and 0.64g of Tween 80 to the mixture, and stir at high speed for 1 hour to obtain an emulsion. Take a small amount of the prepared emulsion with a rubber dropper, drop it on the cover glass, and quickly place it under an optical microscope (model BX51TF. Manufacturer: Olympus Company) connected with a digital camera to take pictures of the morphology of the emulsion. image 3 A, use Photoshop software to image 3 The emulsion particles in A were used for size statistics, and the attached image 3 b.

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Abstract

The hydroxyethyl cellulose of dual template method modifies alginate microballoon as well as its Process for preparing. One is modified microballoon of emulsion template method, mixing hydroxyethyl cellulose solution and sodium alginate aqueous solution, adding liquid paraffin and emulsifier, and then dispersing it in organic oil phase, crosslinking to ball by calcium chloride aqueous solution, further crosslinking in aldehydes crosslinking agent, finally, getting the product after removing liquid paraffin. Another one is modified microballoon of macromolecule template method, dissolving sodium alginate in bovine serum albumin aqueous solution, mixing with hydroxyethyl cellulose and dispersing in organic oil phase, crosslinking to ball by calcium chloride aqueous solution, further crosslinking in aldehydes crosslinking agent, finally getting the product after eluting template molecule by buffer. The getted modified microballoons of the mentioned two methods are simple in technology, getting macroporose microballoon of uniform aperture and the microballoon hasing macromolecule template print effect, and effectively improving the mechanical strength of calcium alginate microballoon and instability in ionic environment.

Description

technical field [0001] The invention relates to polymer microspheres, in particular to double template method hydroxyethyl cellulose modified alginate microspheres and a preparation method thereof. Background technique [0002] Alginate is a kind of natural polysaccharide polymer, which has been widely used in the fields of food, cosmetics and biomedicine. Sodium alginate has attracted much attention due to its gelling effect in the presence of multivalent cations, but its further applications are limited by the instability of ionically cross-linked alginates in electrolyte environments and poor mechanical strength. . For this reason, many researchers try to compound alginate with other polymers to improve application performance. [0003] It has been reported that sodium alginate is mixed with water-based polyurethane to make membranes, combined with cationic polymers, such as chitosan and polylysine, and combined with gelatin to prepare ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C08J3/24C08K3/16C08B11/08G01N33/96G01N33/68
Inventor 成国祥张凤菊英晓光
Owner TIANJIN UNIV
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