Total extractives of steroid saponin, prepn. method and application thereof

A technology of total saponins and extracts, which is applied to the steroid total saponins extract and its preparation and application fields, can solve the problem that the content of active ingredients in the product is not high, the large-scale production and application of ultrasonic extraction is difficult, and the steroids for intravenous injection have not been found. It can achieve the effect of good clinical application value, relieve smooth muscle spasm, and improve the function of vascular endothelial cells.

Inactive Publication Date: 2006-09-13
青岛浩普医药研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Diosgenin Tablets (WS) in National Standard Volume 16 1 -XG-012-2000) is an oral preparation, and the extract prepared by this method cannot be directly used as a raw material for intravenous injection; 3 -B-32) is mainly prepared by water decoction extraction and ethanol precipitation method, and the content of active ingredients in the product obtained by this method is not high; the name is "a kind of freeze-dried powder injection of Chuanshanlong and its preparation method (application number: 200410086729.1)" In the patent application, ultrasonic water extraction, ethanol precipitation, and macroporous resin purification were used to obtain the active ingredients, and pharmaceutical excipients were added to prepare freeze-dried powder injections. In this method, it is difficult to use ultrasonic extraction for large-scale production and application.
At present, there is no literature report about the method and application of steroidal total saponin extract for intravenous injection prepared from pangolin or Dioscorea scutellaria as raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Preparation of pangolin steroidal total saponins extract

[0012] Weigh 10 kg of pangolin, wash with water, slice, add 120 liters of water, decoct for extraction for 1.5 hours, add 80 liters of water to the filter residue, decoct for 1 hour for extraction, and filter. Combine the two filtrates and pass through HPD 100 Type macroporous adsorption resin column, first wash with 2% (weight percent concentration, the same below) sodium carbonate aqueous solution until the effluent is colorless, then wash with water until the effluent is neutral, and finally wash with 75% ethanol aqueous solution until the effluent Colorless, collect the eluent, recover the ethanol in the eluent and concentrate to a relative density of 1.15 (measured at 60°C), add ethanol until the alcohol content reaches 85%, let stand, filter, recover ethanol, concentrate and dry, Pulverize to obtain 170 grams of pangolin steroid total saponin extract, and its total pangoside steroid saponin con...

Embodiment 2

[0013] Embodiment 2: the preparation of total saponins extract of diosgenoid scutellariae

[0014] Weigh 10 kg of Dioscorea scutellaria, wash with water, slice, add 100 liters of water, extract at 80°C for 2 hours, add 80 liters of water to the filter residue, extract at 80°C for 1.5 hours, and filter. Combine the two filtrates and pass through D 101 Type macroporous adsorption resin column, first wash with 3% sodium bicarbonate aqueous solution until the effluent is colorless, then wash with water until the effluent is neutral, and finally wash with 80% ethanol aqueous solution until the effluent is colorless, collect the eluent, After recovering the ethanol in the eluate, concentrate it to a relative density of 1.20 (measured at 60° C.), add ethanol until the alcohol content reaches 90%, let it stand, filter, reclaim the ethanol, concentrate and dry, and pulverize to obtain 208 grams of Dioscorea scutellariae Total body saponin extract, the total saponin content of diosgeno...

Embodiment 3

[0017] Embodiment 3: Preparation of pangolin steroid total saponins sodium chloride injection

[0018] Weigh 2 g of pangolin steroidal total saponins extract, add 9 g of sodium chloride, add water for injection to 1000 ml, adjust the pH value to 7.0 to 7.5 with 10% aqueous sodium hydroxide solution, refrigerate, filter with a G3 vertical melting funnel, and pack, Fill and seal, and sterilize under steam at 100°C for 30 minutes.

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PUM

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Abstract

The invention relates to extract of the steroid saponin produced from the yam or the yam with the guilder leaf which has the character of containing the 80%-90% the steroid saponin in the percentage by weight. The intravenous fluid produced from the extract of the steroid saponin can not only dilate the coronary, depress the myocardial oxygen consumption, melt the thrombus, improve the myocardial ischemia and the microcirculation, amend the function of the vas endothelial cell, increase the blood stream of the brain, prevent the ischemia brain cell injury, but also drop the blood pressure, the blood fat, the blood sugar; at the same time, it has the function of resisting inflammation, ease pain, codein, preventing asthma, dispelling the myospasm, improving the immunity and resisting the allergy. So it can cure the cerebrovascular, the rheumatism and the disease of the respiratory system. The invention has the good clinic extended value because of the low dosage of the active ingredient, the efficacy, the effecting quick and the high bioavailability.

Description

technical field [0001] The invention relates to a steroidal total saponin extract, a preparation method and application thereof. Background technique [0002] The traditional Chinese medicine Chuanshanlong is the dried rhizome of Dioscorea nipponica Makino, another name of Dioscorea nipponica Makino. Mainly produced in Northeast and North China. Dioscorea zingiberensis, a traditional Chinese medicine, is the rhizome of Dioscorea zingiberensis C.H.Wright, a plant of the family Dioscoreaceae. Both Chuanshanlong and Dioscorea scutellariae have the effects of dispelling rheumatism, relieving pain, relaxing tendons and activating blood circulation, relieving cough, relieving asthma and eliminating phlegm. It is clinically used to treat rheumatoid arthritis, lumbago and leg pain, numbness, Kashin-Beck disease, injury from falling, flashback, chronic bronchitis, cough and asthma and other diseases. [0003] Diosgenin Tablets (WS) in National Standard Volume 16 1 -XG-012-2000) i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J75/00A61K31/7048A61P9/10A61P11/00A61P19/02
Inventor 邢雅成
Owner 青岛浩普医药研究有限公司
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