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Cucurbitacin nano preparation comprising protein, preparation method and use thereof

A nano-preparation, cucurbitacin technology, applied in the field of medicine, can solve the problems that have not yet been reported on the research of cucurbitacin protein nanoparticles, and achieve the effects of improving drug efficacy, excellent emulsifying ability, and improving quality

Inactive Publication Date: 2013-01-23
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on cucurbitacin nanoparticles

Method used

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  • Cucurbitacin nano preparation comprising protein, preparation method and use thereof
  • Cucurbitacin nano preparation comprising protein, preparation method and use thereof
  • Cucurbitacin nano preparation comprising protein, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Cucurbitacin BE (effective extraction part of cucurbitacin, cucurbitacin B content greater than 60%) prescription composition: 0.1 g of cucurbitacin, 5 g of human serum albumin, appropriate amount of dichloromethane, and the rest is water for injection, 100 ml in total.

[0021] Preparation Process:

[0022] Weigh the prescribed amount of cucurbitacin, dissolve it with dichloromethane to make a solution, and set aside. Take commercially available human serum albumin (20%) and dilute it with water for injection into a 5% solution, adjust the pH value to 7.5 with acid or alkali, and set aside. Mix cucurbitacin dichloromethane solution and human serum albumin solution at 0°C, emulsify, and prepare colostrum. Then the colostrum is dispersed through a homogenizer (or micro jet), the first step is to adjust the homogenization pressure to 5000psi, and the second step is to adjust to 35000psi, and the average particle size of online detection is 5nm-200nm to obtain ...

Embodiment 2

[0024] Example 2, commercially available cucurbitacin BE (the effective extraction part of cucurbitacin, the content of cucurbitacin B is greater than 60%).

[0025] Prescription composition: 0.01g of cucurbitacin, 1g of human serum albumin, 1g of poloxamer F681, appropriate amount of chloroform, 10g of sucrose, and the rest is water for injection, 100ml in total.

[0026] Preparation Process:

[0027] Weigh the prescribed amount of cucurbitacin, dissolve it with chloroform to make a solution, and set aside. Take 10g of sucrose, dissolve it in water for injection, add 1g each of human serum albumin and poloxamer F68 to make a solution, adjust the pH value to 3 with citric acid, and set aside. Mix cucurbitacin chloroform solution and human serum albumin solution at 10°C, emulsify, and prepare colostrum. Then the colostrum is dispersed through a homogenizer (or micro jet), the first step is to adjust the homogenization pressure to 2000psi, and the second step is to adjust to 3...

Embodiment 3

[0028] Embodiment 3, refined cucurbitacin BE (cucurbitacin B content is greater than 80%)

[0029] Recipe: 0.05g of refined cucurbitacin BE, 0.05g of human serum albumin, 1g of poloxamer, appropriate amount of ethyl acetate, 5g of mannitol, 10g of trehalose, and the rest is water for injection, 100ml in total.

[0030] Preparation process: Weigh the refined cucurbitacin of the prescribed amount, dissolve it with ethyl acetate to make a solution, and set aside. Weigh 0.05 g of human serum albumin and 1 g of poloxamer, dissolve it with water for injection to make a solution, add the prescribed amount of mannitol and trehalose, adjust the pH value to 9 with 0.01M sodium hydroxide after dissolving, and set aside. Mix and emulsify cucurbitacin ethyl acetate solution and human serum albumin solution at 0°C to prepare colostrum. Then the colostrum is dispersed through a homogenizer (or micro-jet), the first step is to adjust the homogenization pressure to 5000psi, and the second ste...

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Abstract

The invention discloses a cucurbitacin nanometer agent with protein, which can be liquid agent or solid agent. The protein nanometer particle can be reserved under indoor temperature, which improves drug quality and effect. the liquid agent contains0.05-10% (W / V) protein quantity and 0.001-1% (W / V) cucurbitacin and the solid agent consists of 50-99.9% (W / V) protein and 0.001-50% (W / V) cucurbitacin.

Description

Technical field: [0001] The invention relates to the technical field of medicine, in particular to a protein-containing cucurbitacin nano-preparation, a preparation method and an application. Background technique: [0002] Cucurbitacins belong to a class of tetracyclic triterpenoids with a 19-methyl group appearing at the C-9 position. They are mainly distributed in Cucurbitaceae plants. It is also found in higher plants such as wood family and some large fungi. Qiu Minghua, a researcher at the Kunming Institute of Botany, Chinese Academy of Sciences, led scientific and technical personnel to study 229 almost all cucurbitacin compounds on the basis of the discovery of a series of new types of cucurbitacin compounds, and proposed a new structural classification model, which was reconstructed from the previous five structural types. Divided into 12 categories, it has been recognized by the international academic community. At present, the cucurbitacin approved for production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61K47/44A61K47/34A61K47/12A61K47/14A61P35/00A61P1/16A61P7/00A61K31/575A61K47/10A61K47/22
Inventor 邓意辉王宁
Owner SHENYANG PHARMA UNIVERSITY
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