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Composition comprising hovenia dulcis thunb. extract, lindera obtusiloba blume extract, or herbal mixture extract thereof

a technology of lindera obtusiloba and hovenia dulcis, which is applied in the direction of biocide, animal repellents, drug compositions, etc., can solve the problems of liver necrosis, mal-function of detoxification, and worsening of liver damage by mental stress

Inactive Publication Date: 2007-07-12
KIM KIYOUNG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016] The composition of the present invention includes an anti-oxidative composition, an anti-fibrosis composition, a liver function improving composition and a kidney function improving composition.
[0021] GOT, GPT, ALP, BUN and total bilirubin, which are all liver function indices, are all lowered in the group administered with Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof, indicating that the extract of the present invention has excellent liver function improving effect. In the meantime, ALP and BUN are not only used as liver function indices but also used as kidney function indices. Thus, the lowered levels of ALP and BUN indicate that Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof of the present invention has excellent kidney function improving effect, as well.
[0022]Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof of the present invention reduces the amount of hydroxyproline in liver tissues but increases that in kidney tissues, indicating that the extract of the invention has excellent anti-fibrosis and kidney protective effects. In particular, the herbal extract extracted from the mixture of Hovenia dulcis Thunb and Lindera obstiloba shows higher effects than each individual extract, that is the mixture extract reduces hydroxyproline in liver tissues more but increases it in kidney tissues more than each individual extract.
[0023]Hovenia dulcis Thunb extract,Lindera obstiloba extract or herbal mixture extract thereof of the present invention can reduce the level of malondialdehyde, an index for lipid peroxidation in liver and kidney tissues, indicating that the extract of the invention has excellent anti-oxidative effect.
[0024] Liver cell line and kidney cell line treated with Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof of the present invention show high cell viability, indicating that the extract of the present invention has excellent liver cell and kidney cell protective effects.
[0025] Therefore, the composition of the present invention can be effectively used for the improvement and the protection of liver and kidney functions and for anti-oxidation and anti-fibrosis as well.

Problems solved by technology

So, excessive mental stress, heavy smoke and alcohol drinking make liver damage worse, resulting in mal-function of detoxification, that is mal-function of immune system, which might be a cause of another disease.
Through such lipid peroxidation, fat is accumulated in liver, protein synthesis is reduced, glycogen is decomposed, and cytoplasmic enzymes in blood vessel are destroyed, resulting in necrosis of liver tissues (Chang I. M., et al., Drug and chemical toxicology 6, 443-453, 1983) Free radical also harms Golgi apparatus, so that protein release out of cells is affected, suggesting that not only liver but also kidney might be damaged.
Thus, one medicine having a certain function is not enough for the treatment of every kinds of liver disease.
The conventional medicine for the treatment of liver diseases has problems of unexpected reaction and other side effects by long-term or mass administration.

Method used

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  • Composition comprising hovenia dulcis thunb. extract, lindera obtusiloba blume extract, or herbal mixture extract thereof

Examples

Experimental program
Comparison scheme
Effect test

experimental example 1

Anti-Oxidative, Anti-Fibrosis, Liver and Kidney Function Improving and Protective Effects of the Extract in Rats Having Liver and Kidney Damage Induced by Long-Term Administration of Carbon Tetrachloride

[0042] Experiments were performed as follows in order to investigate anti-oxidative and anti-fibrosis effects as well as liver and kidney function improving and protective effects of Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof of the present invention in liver and kidney damage models.

[0043] 1. Test Animal

[0044] 12 week old Sprague-Dawley rats weighing about 180˜210 g (Damul science, Osan, Korea) were used as test animals. Breeding temperature was 23±2° C. and relative humidity was maintained as 60±10%. Feed (Purina feed) and water were freely given, and day-night cycle was regulated.

[0045] The animals were adapted to the test room for 2 weeks, and then divided into 5 groups; ① normal group, ② CCl4 treating group, ③ CCl4+herbal mixtur...

experimental example 2

Cytotoxicity Test

[0090] The cell lines used in this invention were NCTC clone 1469 (liver cell line) and vero (kidney cell line), which were purchased from Korea Cell Line Bank (KCLB).

[0091] 5 mg of MTT powder was dissolved in 1 ml of phosphate buffer, which was stirred well and then filtered by 0.4 μm filter, resulting in MTT storage solution.

[0092] 4 mg of NR powder was dissolved in 1 ml of tertially distilled water, which was then stirred well and filtered by 0.4 μm filter, resulting in NR storage solution.

[0093] Trypsin-EDTA containing 0.5% trypsin and 5.3 mM EDTA was purchased and 10-fold diluted with PBS before use.

1) MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] Assay

[0094] After removing medium, 1 ml of trypsin-EDTA solution was added, which was left for 10˜20 minutes. Cells were isolated from the vessel and then transferred to 15 ml falcon tube. 1 ml of medium was added to culture flask and shacked to isolate all the remaining cells, which were a...

example 1

Preparative Example 1

Preparation of Pharmaceutical Compositions

[0106] 1. Preparation of Powders

[0107]Hovenia dulcis Thunb extract (or Lindera obstiloba extract or herbal mixture extract thereof) 2g

[0108] Lactose 1 g

[0109] The above-mentioned ingredients were mixed together, and airtight bag was filled with the mixture to prepare powders.

[0110] 2. Preparation of Tablets

Hovenia dulcis Thunb extract (or Lindera obstiloba100 mgextract or herbal mixture extract thereof)Corn starch100 mgLactose100 mgMagnesium stearate 2 mg

[0111] The above-mentioned ingredients were mixed together, and tablets were prepared by tabletting according to the conventional tablet producing method.

[0112] 3. Preparation of Capsules

Hovenia dulcis Thunb extract (or Lindera obstiloba100 mgextract or herbal mixture extract thereof)Corn starch100 mgLactose100 mgMagnesium stearate 2 mg

[0113] The above-mentioned ingredients were mixed together, and gelatin capsules were filled with the mixture to prepare capsul...

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Abstract

The present invention relates to a composition comprising Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof as an effective ingredient. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention is highly effective for the improvement of liver functions since it can lower the level of GOT, GPT, ALP, BUN and total bilirubin, which are major liver function indices. ALP and BUN are also used as kidney function indices, so the decrease of the level of ALP and BUN by the extract of the present invention indicates that Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the invention can improve kidney functions as well. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention also can lower the amount of hydroxyproline in liver but increase the amount of hydroxyproline in kidney, suggesting that the extract above has excellent anti-fibrosis and kidney protecting effects. In addition, Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention can lower the level of malondialdehyde, an index of lipid peroxidation in liver and kidney tissues, suggesting that the extract has excellent anti-oxidative effect. Hovenia dulcis Thunb extract, Lindera obtusiloba extract or herbal mixture extract thereof of the present invention promotes cell viability in liver and kidney cell lines, indicating that the extract has excellent liver and kidney cell protective effects. Therefore, the composition of the present invention can be effectively used not only for anti-oxidation and anti-fibrosis but also for the protection and the improvement of liver and kidney functions.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition comprising Hovenia dulcis Thunb extract, Lindera obstiloba extract or herbal mixture extract thereof as an effective ingredient. BACKGROUND ART [0002] As one of representative adult diseases, liver disease is caused by liver damage resulted from chronic fatigue by stress and most of exogeneous substances. The development rate of liver disease in Korea is very high, comparing to foreign countries, and in particular, the death rate of liver cancer is the top in the world and the death rate of chronic liver disease is the third. According to a recent report from National Statistical Office, Korea, liver disease is the leading cause of death of adults at the age of 40s in Korea. Among many liver diseases, the most fatal disease is viral hepatitis in Korea. In the meantime, the death by liver cirrhosis is 5˜10 fold higher than that by viral hepatitis in Western countries. [0003] Liver is the organ that shows the most a...

Claims

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Application Information

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IPC IPC(8): A61K36/72A23L33/00A61K36/54
CPCA23L1/3002A61K36/54A61K36/72A61K2300/00A23L33/105A61P1/16A61P13/12A61P17/18
Inventor KIM, KIYOUNG
Owner KIM KIYOUNG
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