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Peptides Having Activities of Insulin Like Growth Factor-1 and their Uses

a technology of growth factor and activity, applied in the field of peptides having insulin like growth factor1, can solve the problems of complex purification, time-consuming and cost-intensive refolding process, and the lik

Inactive Publication Date: 2009-07-02
CAREGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0031]SEQ ID NO:4 corresponds to amino acids 52-61 of IGF-1. Preferably, in the amino acid sequence of SEQ ID NO:4, Cys residues at the N- and C-terminal are linked to form a disulfide bond, producing a cyclized peptide for enhancing the stability of peptides.
[0034]The modifications of peptides described above greatly increase the stability of peptides of this invention. The term used herein “stability” refers to in vivo stability and storage stability (e.g., storage stability at room temperature) as well. The protection group described above protects the peptides from the attack of protease in vivo.
[0039]Where the present composition is applied to periodontal diseases, it may be formulated into toothpaste or a composition for tooth and mouth cleaning or caring. The term “composition for treating periodontal diseases” may be interchangeably used herein with other terms, “composition for tooth and mouth caring” and “composition for tooth and mouth cleaning”. The peptide of this invention promotes biological activities of epidermal cells present in gum tissues and heals gum wound to regenerate damaged gum tissues, thereby treating or preventing periodontal diseases.
[0041]For example, the peptides used as active ingredients in the present composition promote the proliferation of keratinocytes, induce the biosynthesis of procollagen, laminin and fibronectin to regenerate keratinocyte layer, epidermis and dermis, thereby resulting in the improvements in wrinkle, skin elasticity and skin moisture, the prevention of skin aging, wound healing, skin regeneration and gum tissue regeneration.
[0060](ii) the peptides of the present invention is much higher stability and skin penetration potency than natural-occurring IGF-1;

Problems solved by technology

However, these preparations are encountered to need of time- and cost-consuming refolding process and of complex purification process to remove E. coli-originated contaminants.

Method used

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  • Peptides Having Activities of Insulin Like Growth Factor-1 and their Uses
  • Peptides Having Activities of Insulin Like Growth Factor-1 and their Uses
  • Peptides Having Activities of Insulin Like Growth Factor-1 and their Uses

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

Tyr-Phe-Asn-Lys-Ala-Ala-Gly-Tyr-Gly-Ser-Ser-Ser-Arg-Arg (SEQ ID NO:1)

[0079]To 700 mg of chloro trityl chloride resin (CTL resin, Nova Biochem Cat No. 01-64-0021) introduced into a reactor were added 10 ml of methylene chloride (MC) and agitated for 3 min. After removing solution, 10 ml of dimethylformamide (DMF) were added to the resultant and then agitation was carried out for 3 min, after which the solvent was removed. 10 ml of dichloromethane solution were added to the reactor and 200 mmole of Fmoc-Arg(pbf)-OH and 400 mmole of diisopropyl ethylamine (DIEA) were then added to the reactor, after which the mixture was dissolved by agitation and reaction was then undertaken with agitating for 1 hr. After reaction, the resultant was washed and reacted for 10 min in methanol and DIEA (2:1) dissolved in DCM, followed by washing with excess DCM / DMF (1:1). Following the removal of the solvent, 10 ml of DMF were added to the reactor and agitated for 3 min. followed by removing the solvent...

preparation example 2

ptides

[0080]The peptides of SEQ ID NOs:2-5 were synthesized as processes described in Preparation Example 1. SEQ ID NO:2 (Gly-Tyr-Gly-Ser-Ser-Ser-Arg-Arg-Ala-Pro-Gln-Thr: GYGSSSRRAPQT) corresponds to amino acids 30-41 of IGF-1, SEQ ID NO:3 (Glu-Ser-Ser-Phe-Arg-Ser-Ser-Asp-Leu-Arg-Arg-Leu: ESSFRSSDLRRL) to amino acids 46-57 of IGF-1, SEQ ID NO:4 (Cys-Asp-Leu-Arg-Arg-Leu-Glu-Met-Tyr-Cys: CDLRRLEMYC) to amino acids 52-61 of IGF-1, and SEQ ID NO:5 (Arg-Arg-Leu-Glu-Met-Tyr-Cys-Ala-Pro-Leu-Lys-Pro: RRLEMYCAPLKP) to amino acids 55-66 of IGF-1.

[0081]The determined molecular weights of the peptides are summarized in Table 1.

TABLE 1Analyzed values(mass analyzer)SEQ IDAmino acidAnalyzedTheoreticalNOsequencevaluesvalues1GFYFNKAAGYGSSSRR1770.61767.92GYGSSSRRAPQT1270.881266.43ESSFRSSDLRRL1457.41452.64CDLRRLEMYC1304.81303.65RRLEMYCAPLKP1479.51476.8

preparation example 3

Leu-Arg-Arg-Leu-Glu-Met-Tyr-Cys (1,10 Cyclized CDLRRLEMYC)

[0082]To increase the stability of the peptide of SEQ ID NO:4 prepared in Preparation Example 2, the cyclization was carried out using Cys residues at the C- and N-terminal. 100 mg of the peptide of SEQ ID NO:4 (Cys-Asp-Leu-Arg-Arg-Leu-Glu-Met-Tyr-Cys-OH) were dissolved in 1 L of 10% DMSO / deprotection distilled water. After fixing pH to 8.0, the peptide solution was agitated for 8 hr under air to induce oxidation. By preparative chromatography, 40 mg of cyclized Cys-Asp-Leu-Arg-Arg-Leu-Glu-Met-Tyr-Cys-OH (1,10 cyclized). The molecular weight of the final cyclized peptide was determined as 1302.8 using a mass analyzer.

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Abstract

The present invention relates to a peptide having the activity of insulin like growth factor-1 (IGF-1) and derived from IGF-1, a composition for improving skin conditions or treating a periodontal disease comprising the peptide. The IGF-1 mimicking peptides of this invention have identical functions or actions to natural-occurring IGF-1 and much better stability and skin permeation than natural-occurring IGF-1. In these connections, the composition comprising the peptides of this invention can exhibit excellent efficacies on the treatment, prevention and improvement of diseases or conditions demanding IGF-1 activities. In addition, the peptides of this invention can be advantageously applied to pharmaceutical compositions, quasi-drugs and cosmetics.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to peptides having insulin like growth factor-1 (IGF-1) and theirs uses.[0003]2. Description of the Related Art[0004]Insulin like growth factor-1 (IGF-1) with a molecular weight of 7,649 Da consisting of 70 amino acids is generated by human growth hormone in the liver and then secreted in blood. Its amino acid composition shows about 43% similarity to A chain of insulin. IGF-1 exhibiting similar actions to insulin is capable of binding to insulin receptor as well as its receptor, and promoting cell proliferation. IGF-1 is usually called somatomedin C due to its growth mediation activity.[0005]It has been reported that the IGF-1 receptor is present in various tissues such as adipose tissue, lymphocyte, bond and placental membrane as well as hepatocyte. IGF-1 receptor has been revealed to have other signaling pathway than insulin receptor. The binding of IGF-1 to its receptor results in promo...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K8/64C07K7/08A61K38/10A61P17/00A61P17/14A61P17/10A61P17/18A61P17/02A61Q11/00
CPCC07K14/65A61K38/00A61P1/02A61P17/00A61P17/02A61P17/10A61P17/14A61P17/16A61P17/18
Inventor CHUNG, YONG JIKIM, YOUNG DEUGKIM, EUN MI
Owner CAREGEN
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