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Poultry farm practices

a poultry farm and farm technology, applied in the field of poultry farming facilities, can solve the problems of slow feeding, low weight, disease in the poultry population, etc., and achieve the effects of improving the environment, improving the environment, and improving the environmen

Inactive Publication Date: 2015-02-05
GLOBAL BIOPROTECT IP PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about improving the environment in poultry farming facilities by controlling the production of ammonia. This is accomplished by reducing or controlling bacteria that produce urease, which breaks down urea to ammonia. The reduction of ammonia is achieved by using biosurfactants, specifically cyclic lipopeptides, which are produced by modulating growth conditions and nutrients. These biosurfactants have varying ratios of lipid fatty acid chain lengths, which can be tailored based on the desired application. Overall, this invention helps to create a safer and more efficient poultry farming environment.

Problems solved by technology

However, these facilities have production problems that are deleterious to bird health and result in slower feeding, low weight and / or cause disease in the bird population.
This results in gastrointestinal pathogens spreading quickly through a facility.
However, in recent years some countries, such as European countries, have banned the use of antibiotics that are also used in humans, because of the emergence of antibiotic resistant pathogenic bacteria.
High levels of ammonia in the facilities is deleterious to bird health and industry regulations require ammonia levels in mass production poultry facilities to be kept below 25 ppm.
However, these neutralizing chemicals are only active during initial production and are consumed by about day 10 of production.
Another difficulty with the use of acidic neutralizing chemicals is that they are in constant contact with the feet of the birds and cause or exacerbate pododermatitis caused by Staphylococcus infection and blistering of their feet.
Such compositions are also not indicated as suitable for large scale use in environments such as mass production poultry farms.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Production of Surfactin Biosurfactant

[0099]Bacillus subtilis NRRL B-3383 strain (originally obtained from the United States Department of Agriculture) from bacterial culture was transferred at a 2% volume by volume inoculum into 4 L shake flasks containing 2.5 L of 10% vinasse based MMS broth. The vinasse based MMS broth containing:

componentquantityvinasse100mLammonium nitrate4.1gsodium phosphate dibasic5.68gtetrasodium tetrahydrate EDTA1.8mgmanganese sulfate6.8mgautoclaved deionized waterto 1L

[0100]The flasks were placed on orbital shakers (SKC 6100, Jeio Tech) at 150 rpm while incubating at 30° C. (MCO-801C Incubator, Sanyo). After 72 hours, flasks were removed from the incubator and the biomass removed from the crude culture broth by centrifugation at 8,500 rpm for 20 min at 4° C. (Sorvall Evolution RC).

[0101]The pH of the resulting supernatant was brought to a pH of 2.0 using HCl which resulted in precipitation of surfactin and the supernatant stored overnight at 4° C. to ensure...

example 2

Production of Blends of Surfactin and Lichenysin

[0104]Bacillus subtilis and Bacillus licheniformis were used to inoculate 4 L shake flasks containing 10% molasses based MMS broth. The molasses based MMS broth containing:

componentquantitymolasses100mLammonium nitrate4.1gsodium phosphate dibasic5.68gtetrasodium tetrahydrate EDTA1.8mgmanganese sulfate6.8mgautoclaved deionized waterto 1L

[0105]The flasks were placed on orbital shakers (SKC 6100, Jeio Tech) at 150 rpm while incubating at 30° C. (MCO-801C Incubator, Sanyo). After 72 hours, flasks were removed from the incubator and the biomass removed from the culture broth by centrifugation at 8,500 rpm for 20 min at 4° C. (Sorvall Evolution RC).

example 3

[0106]In a 1 liter bioreactor a buffer solution was prepared containing 800 mL demineralized water, monopotassium phosphate (20.4 g), disodium phosphate (28.4 g) and yeast extract (5 g). The buffer was mixed and the pH adjusted to 7. The buffer was then autoclaved. In a 1 L bioreactor, 200 mL of the buffer solution was mixed with magnesium sulfate (2 mL of 12 g / 100 mL solution), calcium chloride (1 mL, 0.1 M), ferrous sulfate heptahydrate (1 mL, 15.7 g / 100 mL solution), manganese sulfate monohydrate (1 mL, 3.8 g / 100 mL solution), sodium EDTA (1 mL, 0.18 g / 100 mL solution) and glucose (20 mL, 20 g / 80 g deionized water) and ammonium nitrate (10 mL, 82 g / 200 mL solution). Bacillus species spores were inoculated into the bioreactor at 2% wt. The biomass was agitated at 300 rpm for 12 hours at 35° C. The Bacillus was incubated until the cell density was to 1×1014 cfu per mL and the OD600 nm was 2.2.

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Abstract

The present invention relates to methods of improving the environment within a poultry farming facility including reducing ammonia production in a poultry facility, inhibiting urease enzymes in poultry litter, reducing levels of pathogenic bacteria in poultry litter, improving productivity of poultry farms, reducing or preventing pododermatitis in poultry reared in mass production poultry facilities and controlling pests in poultry litter. Compositions, suitable for use in such methods, comprising at least one microorganism of the genus Bacillus and at least one biosurfactant wherein the biosurfactant is present in an amount of 2 mg / L to 7000 mg / L are also described.

Description

FIELD OF THE INVENTION[0001]The present invention relates to methods of improving the environment within a poultry farming facility including reducing ammonia production in a poultry facility, inhibiting urease enzymes in poultry litter, reducing levels of pathogenic bacteria in poultry litter, improving productivity of poultry farms, reducing or preventing pododermatitis in poultry reared in mass production poultry facilities and controlling pests in poultry litter. Compositions, suitable for use in such methods, comprising at least one microorganism of the genus Bacillus and at least one biosurfactant wherein the biosurfactant is present in an amount of 2 mg / L to 7000 mg / L are also described.BACKGROUND OF THE INVENTION[0002]To provide food for an increasing population, the total production of grains, meats, vegetables and dairy products has increased 70% in the past 50 years. Correspondingly, the growth of the poultry industry has dramatically increased. The US value of production...

Claims

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Application Information

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IPC IPC(8): A01N63/02C12R1/125A01N63/00A01N63/22
CPCA01N63/02A01N63/00C12R1/125C05F5/006C07K14/32C09K8/584C12P21/02C05F11/08A61P17/00A61P31/04Y02A40/20A01N63/22C12N1/20A01N63/10C12R2001/125C12N1/205C12R2001/12A01N37/18C07K7/64
Inventor BRALKOWSKI, MICHAEL PAULBROOKS, SARAH ASHLEYHINTON, STEPHEN M.WRIGHT, DAVID MATTHEWYANG, SHIH-HSIN
Owner GLOBAL BIOPROTECT IP PTY LTD
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