Microbial compositions for use in combination with soil insecticides for benefiting plant growth

a technology of soil insecticides and compositions, which is applied in the direction of applications, biocide, waste based fuel, etc., can solve the problems of reducing the economic benefits of soil insecticides, stimulating plant growth, and slowing fruit ripening,

Inactive Publication Date: 2016-06-30
FMC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, these microorganisms can interfere with a plant's ethylene stress response by breaking down the precursor molecule, 1-aminocyclopropane-1-carboxylate (ACC), thereby stimulating plant growth and slowing fruit ripening.
(e.g. P. pachyrhizi), are one type of plant pest that can cause severe economic losses in the agricultural and horticultural industries.
Chemical agents can be used to control fungal phytopathogens, but the use of chemical agents suffers from disadvantages including high cost, lack of efficacy, emergence of resistant strains of the fungi, and undesirable environmental impacts.
In addition, such chemical treatments tend to be indiscriminant and may adversely affect beneficial bacteria, fungi, and arthropods in addition to the plant pathogen at which the treatments are targeted.
(such as R. soleacearum) that cause severe economic losses in the agricultural and horticultural industries.
A final type of plant pathogen includes plant pathogenic nematodes and insects, which can cause severe damage and loss of plants.

Method used

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  • Microbial compositions for use in combination with soil insecticides for benefiting plant growth
  • Microbial compositions for use in combination with soil insecticides for benefiting plant growth
  • Microbial compositions for use in combination with soil insecticides for benefiting plant growth

Examples

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example 1

Identification of a Bacterial Isolate as a Bacillus Pumilus Through Sequence Analysis

[0116]A plant associated bacterial strain, designated herein as RTI279, was isolated from the rhizosphere soil of merlot vines growing at a vineyard in NY. The 16S rRNA and the rpoB genes of the RTI279 strain were sequenced and subsequently compared to other known bacterial strains in the NCBI and RDP databases using BLAST. It was determined that the 16S RNA sequence of RTI279 (SEQ ID NO: 1) is identical to the 16S rRNA gene sequence of eight other strains of B. pumilus, including B. pumilus SAFR-032. This confirms that RTI279 is a B. pumilus. It was determined that the rpoB gene sequence of RTI279 (SEQ ID NO: 2) has the highest level of sequence similarity to the gene in the B. pumilus SAFR-032 strain (i.e. 99% sequence identity); however, there is a 47 nucleotide difference on the DNA level, indicating that RTI279 is a new strain of B. pumilus.

example 2

Genes Related to Osmotic Stress Response in RTI279 Bacillus Pumilus

[0117]Further sequence analysis of the genome of Bacillus pumilus strain RTI279 revealed that this strain has genes related to osmotic stress response, for which there are no homologues in the other closely related B. pumilus strains. This is illustrated in FIG. 1, which shows a schematic diagram of the genomic organization surrounding and including the osmotic stress response operon found in Bacillus pumilus RTI279. In FIG. 1A, the top set of arrows represents protein coding regions for the RTI279 strain with relative direction of transcription indicated. For comparison, the corresponding regions for two Bacillus pumilus reference strains, ATCC7061 and SAFR-032, are shown below the RTI279 strain. Genes are identified by their 4 letter designation unless no designation could be found. If no designation could be found, the gene abbreviations are indicated in the legend shown in FIG. 1B. The degree of amino acid ident...

example 3

Growth Effects of Bacillus Pumilus Isolate RTI279 on Wheat

[0119]The effect of application of the bacterial isolate on early plant growth and vigor in wheat was determined. The experiment was performed by inoculating surface sterilized germinated wheat seeds for 2 days in a suspension of 10+7 bacterial cfu / ml at room temperature under shaking (a control was performed without bacterial cells). Subsequently, the control and inoculated seeds were planted in 4″ pots in duplicate in sand mixture. Each pot was seeded with five seeds of wheat variety HARD RED at 1-1.5 cm depth. Pots were incubated in growth chamber at 24° C. / 18° C. with light and dark cycle of 14 / 10 hrs and watered as needed for 13 days. Dry weight was determined as a total weight per 10 seeds resulting in a total weight equal to 363 mg for the plants inoculated with the RTI279 strain versus a total weight equal to 333.8 mg for the non-inoculated control which is an 8.7% increase in dry weight over the non-inoculated contro...

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Abstract

Compositions and methods are provided for benefiting plant growth. The compositions contain isolated bacterial or fungal strains having properties beneficial to plant growth and development that can provide beneficial growth effects when delivered in a liquid fertilizer in combination with a soil insecticide to plants, seeds, or the soil or other growth medium surrounding the plant or seed. The beneficial growth effects include one or a combination of improved seedling vigor, improved root development, improved plant health, increased plant mass, increased yield, improved appearance, improved resistance to osmotic stress, improved resistance to abiotic stresses, or improved resistance to plant pathogens. The isolated bacterial strains include those of the Bacillus species including species such as Bacillus pumilus, Bacillus licheniformis, and Bacillus subtilis.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. provisional application No. 62 / 097,198 filed Dec. 29, 2014 and U.S. provisional application No. 62 / 171,582 filed Jun. 5, 2015, the disclosures of which are each hereby incorporated herein by reference in their entireties.TECHNICAL FIELD[0002]The presently disclosed subject matter relates to compositions and products comprising isolated microbial strains and methods of use thereof to benefit plant growth.BACKGROUND OF THE INVENTION[0003]A number of microorganisms having beneficial effects on plant growth and health are known to be present in the soil, to live in association with plants specifically in the root zone (Plant Growth Promoting Rhizobacteria “PGPR”), or to reside as endophytes within the plant. Their beneficial plant growth promoting properties include nitrogen fixation, iron chelation, phosphate solubilization, inhibition of non-beneficial microrganisms, resistance to pests, Induced S...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/00C05G3/02A01N53/00A01N63/22C05G3/60
CPCA01N63/00C05G3/02A01N53/00C05G3/60Y02A40/10Y02A50/30Y02E50/30Y02W30/40A01N63/22A01N37/46A01N51/00
Inventor TAGHAVI, SAFIYHVAN DER LELIE, DANIELWALMSLEY, MARK R.CALDWELL, NATHANANDERSON, THOMAS E.SPADAFORA, VINCENT J.BUCKELEW, LAMAR
Owner FMC CORP
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