The invention provides a
cell activation solution, a preparation method and application of the
cell activation solution in preparation of a
cell radiation damage protective preparation, and belongs to the technical field of
radiation protection organic
selenium preparations. The preparation method comprises the following steps: pretreatment of
cardamine violifolia, extraction of organic
selenium,
glycosylation and nanocrystallization. The method for extracting the organic
selenium comprises the following steps: adding
cardamine violifolia
powder into deionized water, carrying out ultrasonic treatment at 45-55 DEG C for 20-30 minutes, adjusting the pH value to 8-9, adding
alkaline protease, stirring and carrying out enzymolysis for 3.5-4.5 hours, inactivating the
alkaline protease at 90-95 DEG C, then centrifuging and collecting supernate, adding absolute ethyl
alcohol into the supernate, standing at 3-5 DEG C for 15-20 hours, centrifuging again, and collecting precipitate, so as to obtain the organic selenium. According to the
cell activation liquid, in a mouse experiment, the
survival rate of the group A after 30 days is 70-76%, the in-vivo
lymphocyte decline rate after 24 hours of
radiation is 35.8-39.6%, the
survival rate of the group B after 30 days is 68-70%, and the in-vivo
lymphocyte decline rate after 24 hours of radiation is 44.7-46.5%.