Heterogenic deproteinized osseous stent material and its preparation method
A bone scaffold, xenogeneic technology, applied in bone implants, medical science, prostheses, etc., can solve the problems of affecting biomechanical strength, material fragility, etc., to eliminate immunogenicity, low cost, good mechanical strength and performance effect
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Embodiment 1
[0026] Example 1: Analysis of the physicochemical properties of the heterogeneous deproteinized bone of the present invention
[0027] Histology, scanning electron microscopy, infrared spectroscopy, X-ray diffraction analysis, X-ray energy scattering analysis, trace Kjeldahl nitrogen determination and mechanical properties were performed on the prepared heterogeneous deproteinized bone. The results of these observations and experimental analysis are described below.
[0028] 1. Gross and histological observation: the prepared deproteinized bone is white columnar (about 10mm×5mm×3mm in size), with a porous honeycomb structure visible to the naked eye, hard texture, and no peculiar smell. HE staining showed that the deproteinized bone trabecular structure was clear, the stroma and cells had been removed, and the layered structure of the cortical bone plate was intact; while the fresh bone showed stroma and cells. Deproteinized interstitial collagen fibers stained positively, wh...
Embodiment 2
[0039] Example 2: Biosafety study of heterologous deproteinized bone of the present invention
[0040] The purpose of this example is to use standard methods such as acute and subacute toxicology tests, hemolysis tests, heat source tests, intradermal injection tests, muscle implantation tests, and cytotoxicity tests to evaluate the effect of the heterogeneous deproteinized bone implanted in the body. biosecurity. The results of these experiments are briefly described below.
[0041] 1. Acute and subacute toxicology test: During the mouse observation period, the recipient mice had a normal appetite, were full of energy, moved freely, and had no adverse reactions such as respiratory depression and shortness of breath. After 72 hours the animals gained significant body weight. There was no ascites in the anatomical observation, and there was no adhesion in the abdominal cavity. HE staining showed no abnormality in the liver, spleen, kidney and other organs.
[0042] 2. Hemolys...
Embodiment 3
[0053] Example 3: Cytocompatibility study of heterogeneous deproteinized bone of the present invention
[0054] This example describes the results of a cytocompatibility study of xenogeneic deproteinized bone.
[0055] After the compound culture of heterogeneous deproteinized bone and bone marrow stromal cells of the present invention, the adherent growth state was observed by inverted phase contrast microscope and scanning electron microscope, and judged by MTT method, flow cytometry and alkaline phosphatase (ALP) activity detection The survival rate, differentiation level, vitality and metabolic level of the transplanted cells.
[0056] 1. Inverted Phase Contrast Microscopy and Histological Observation
[0057] After the deproteinized bone and bone marrow stromal cells were cultured for 24 hours, the cells could adhere and distribute on the surface of the material and in the pores. With the prolongation of the culture time, the cells further grew, differentiated and prolife...
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