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Method of utilizing rumen microbe in degrading waste plant matter effectively

A technology of rumen microorganisms and plant matter, applied in the biological field, can solve the problems of wasting resources, overflowing the sky, polluting the environment, etc., and achieve the effect of improving degradation efficiency and low manufacturing cost

Inactive Publication Date: 2009-11-04
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country produces a large amount of plant waste every year. For example, the annual output of various crop straws in rural areas is more than 600 million tons. Most of the current treatment methods are incineration on the spot, which often results in smog and seriously pollutes the environment. , also wasting precious and limited resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Collect the harvested corn stalks, dry them in the sun for 5 days, and use a grinder to crush the stalks into particles with a diameter less than 5mm.

[0013] 2. Take 300ml of rumen fluid from the fistula goat and quickly transfer it to a 5-liter fermenter, add 80g of corn stalk granules and 3.7L of mineral buffer solution, and maintain the pH of the fermenter at 6.0-6.4 by adjusting the concentration of sodium hydroxide in the buffer solution , a temperature of 32°C, and a stirring rate of 60 rpm. The addition rate of straw powder was kept at 30g / L / d, the residence time of the buffer in the reactor gradually decreased from 24 hours to 18 hours on the 6th day and remained unchanged, and the residence time of straw particles in the reactor was 60 Hour. The composition of the components in the produced gas was detected every day. When there were only hydrogen and carbon dioxide in the gas components and no methane was detected for two consecutive weeks, the methanoge...

Embodiment 2

[0016] 1. Collect household garbage and remove inorganic substances contained in it, such as stones, iron filings, etc. After 5 days in the cool sun, it was pulverized into particles smaller than 6mm.

[0017] 2. Take 700ml of rumen fluid from the fistula goat and quickly transfer it to a 5-liter fermenter, add 80g of crushed plant matter organic waste and 3.3L of mineral buffer solution, and maintain the pH in the fermenter by adjusting the concentration of sodium hydroxide in the buffer solution. 6.3-6.7, the temperature is 37°C, and the stirring rate is 90 rpm. The addition rate of the pulverized plant matter organic waste remained at 40g / L / d, and the residence time of the buffer solution in the reactor gradually decreased from 24 hours to 12 hours on the 6th day and remained constant. The residence time in the reactor was 60 hours. The composition of the components in the produced gas was detected every day. When there were only hydrogen and carbon dioxide in the gas com...

Embodiment 3

[0020] 1. Harvest fresh cattail grass from the pond, and use a beater to break it into a slurry with a length of less than 8mm.

[0021] 2. Take 1200ml of rumen juice from the fistula goat and quickly transfer it to a 5-liter fermenter, add 80g of pulpy cattail grass and 2.8L of mineral buffer, and maintain the pH in the fermenter at 6.3- by adjusting the concentration of sodium hydroxide in the buffer. 6.8, the temperature is 41°C, and the stirring rate is 100 rpm. The addition rate of pulpy cattail grass remains at 20g / L / d, and the residence time of the buffer solution in the reactor gradually drops from 24 hours to 16 hours on the 6th day and remains unchanged, and the residence time of cattail grass in the reactor is 60 Hour. The composition of the components in the produced gas was detected every day. When there were only hydrogen and carbon dioxide in the gas components and no methane was detected for two consecutive weeks, the methanogenic bacteria were completely elim...

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Abstract

The invention relates to biotechnology for plant waste treatment and resource utilization. Including (1) crushing or beating plant waste into a spare base material, (2) removing rumen microorganisms from the rumen of ruminants and inoculating them into an anaerobic digestion reaction containing plant waste base material and mineral buffer In the device, (3) start the operation of the anaerobic digestion reactor to hydrolyze and acidify the plant matter, and (4) collect the gas, liquid and solid phase products produced. The rumen microorganisms must first be inoculated into the fermenter for cultivation and domestication to remove the methanogens therein, until no methane can be detected in the gas produced for two consecutive weeks, and then inoculated into the anaerobic digestion reactor to enter the routine operation procedure . The hydrogen ions released during the degradation process of the present invention are all released in the form of hydrogen gas without generating methane, which is favorable for protecting the environment. The liquid phase product is a short-chain organic acid, which is the raw material for hydrogen production; the solid phase product is rich in protein, which can be treated as an organic fertilizer. The invention produces the effect of turning waste into wealth.

Description

Technical field: [0001] The invention relates to biotechnology for plant waste treatment and resource utilization. Background technique: [0002] my country produces a large amount of plant waste every year. For example, the annual output of various crop straws in rural areas is more than 600 million tons. Most of the current treatment methods are incineration on the spot, which often results in smog and seriously pollutes the environment. , but also a waste of precious and limited resources. In cities, people's domestic garbage is also the main source of pollution in the city, and there are also a large amount of plant waste in domestic garbage. From the perspective of protecting the environment, how to deal with and utilize these plant wastes is a very hot topic nowadays. [0003] Existing treatment techniques include chemical methods and biotechnology methods. Among them, biotechnology methods are valued because of their environmental friendliness and low cost. The Bri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09B3/00
Inventor 俞汉青胡真虎
Owner UNIV OF SCI & TECH OF CHINA
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