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Method for quantum dots namo fluorescence probe combined with biochip to find Chinese medicine target

A nano-fluorescent probe and bio-chip technology, applied in the intersection of chemical industry, materials, pharmacy and biology, can solve the requirements of low fluorescence quantum yield, no quantum dot nanotechnology to find traditional Chinese medicine targets, and optical part of detection system Strict and other problems, to achieve the effect of broad application prospects

Inactive Publication Date: 2007-09-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, this fluorescent dye has relatively large defects, such as low fluorescence quantum yield; Fluorescent probes are excited with the same excitation light, etc.
[0008] Although protein chip technology has been relatively successfully used in high-throughput drug screening ( Our Protein Core Chip technology successfully applied to high-throughput drug screening .http: / / www.ebiotrade.com / newsf / 2005-4 / 2005421100624.htm), but there is no experimental report on combining quantum dot nanotechnology with protein chip technology to find Chinese medicine targets

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Add 300 μl of 27% (v / v) dehydrated ethanol (purchased from Shanghai East) to 50 ml of CdSe (590) (self-made) (self-made) with a concentration of about 10 nM (590 means that the peak position of the emission spectrum of CdSe is located at 590 nm) nanoparticle aqueous solution. Yi Chemical Reagent Company) mixed solution of tetraethyl orthosilicate (purchased from China Pharmaceutical Group Chemical Reagent Company) and 20 μl of ammonia water, after 2-3 days of reaction, a clear and transparent solution was obtained, and some samples were freeze-dried and subjected to X- X-ray diffraction (XRD) and X-ray energy spectrum (XPS) analysis, the results show that the surface of CdSe has been coated with silicon dioxide, that is, the formation of CdSe / SiO 2 . (in the preparation of CdSe / SiO 2 The amount of tetraethyl orthosilicate, absolute ethanol and ammonia water should be controlled, otherwise it will cause particle aggregation. )

[0023] CdSe / SiO 2 Add to absolute etha...

Embodiment 2

[0025] Salvianolic acid B (Jiangxi Bencao Tiangong Technology Co., Ltd.) was dissolved in PBS buffer at pH 7, and 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC ) (purchased from China Pharmaceutical Group Chemical Reagent Company), reacted at room temperature for 1 h, then added quantum dot CdSe / ZnS(590) (self-made) aqueous solution of nanoparticles modified on the surface of mercaptoethylamine, and reacted overnight at room temperature, through the covalent The reaction links the quantum dots to the drug. In the reaction solution, CdSe / ZnS is about 50nM, and the molar ratio of CdSe / ZnS, salvianolic acid B and EDC is about 1:100:120. The free salvianolic acid B and EDC were removed by gel column separation, and the connection product of "CdSe / ZnS-salvianolic acid B" was obtained. The product emits the same fluorescence as CdSe / ZnS, ie red fluorescence, when excited by light with a wavelength of 488nm. Capillary electrophoresis experiments showed that the ...

Embodiment 3

[0027] Co-incubation of "quantum dot-salvianolic acid B" nanoprobe and protein chip: the protein chip in the kit (Shukang Biotechnology Co., Ltd.) was used in this work. Although this chip was not specially designed for this experiment, its A variety of specific antigens are immobilized on the surface, and they are expressed on the surface of normal cells and tumor cells, especially on the surface of tumor cells. The experiment was performed according to the method of the kit. The "quantum dot-salvianic acid B" nanoprobe solution and the chip were incubated at 37°C for 30-40min, washed, and analyzed by confocal laser scanning. It was found that there were a few fluorescent spots on the chip, indicating that salvianolic acid B may act on various surface antigens of tumor cells.

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PUM

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Abstract

This invention supply a method of quanta point nanometer technology combine with biochip technology to search Chinese traditional medicine effective ingredient action target spot, belong to stuff, chemical engineering, pharmacy and biologic cross region. This invention connect quanta point with Chinese traditional medicine effective ingredient, form a 'quanta point - Chinese traditional medicine effective ingredient' fluorescence probe, then put fluorescence probe directly altogether incubate with biochip, after washing, base on fluorescence emit from biochip 's quanta point to ascertain idiosyncratic combine LOCA( namely target spot) between medicament and chip's fixed given protein, then through computer software to analyze protein of these combined LOCA . This invention can easily and rapidly search target protein of Chinese traditional medicine effective ingredient create pharmacodynamic to cell, can supply high duty technology instrument for Chinese traditional medicine study and new drug screening.

Description

technical field [0001] The invention belongs to the interdisciplinary field of materials, chemical engineering, pharmacy and biology, and relates to a method for finding the action target of traditional Chinese medicine through the combination of nanotechnology and biochip technology. Components act on the target protein approach. Background technique [0002] Traditional Chinese medicine has a long history in our country. She has condensed the traditional culture of the Chinese nation for thousands of years and has made great contributions to the health and civilization of the Chinese nation and even the people of the world. Various Chinese herbal medicines and their formulas recorded in medical literature such as "Huangdi Neijing", "Qianfangjing" and "Compendium of Materia Medica" handed down for thousands of years still have very precious reference value in today's highly developed medical science and technology. [0003] However, my country's traditional Chinese medicin...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/50C09K11/00
Inventor 储茂泉
Owner TONGJI UNIV
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