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Method for extracting and purifying flavonoids from apple powder agent and application thereof

A technology of apple flavonoid powder and purification method, which is applied in the chemical field, can solve the problems of the separation result and yield being greatly influenced by the resin adsorption, the effect of repeated utilization is unsatisfactory, the regeneration is cumbersome, and the like, and the chemical properties are stable and delayed Aging, non-irritating effect on skin

Active Publication Date: 2007-12-12
上海利盛生化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the macroporous resin is regenerated, the separation result and yield are greatly affected by the adsorption of the resin, the regeneration is more cumbersome, and the effect of reuse is not satisfactory.
Wild apple flavonoids can be used as antioxidants in food and health food, but it is not mentioned whether it can be used in the field of cosmetics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 10 kg of apple pomace, put it in a multi-functional extraction tank, add 100 liters of ethanol solution with a mass concentration of 30%, heat it to 100° C., stir and extract for 0.5 hours, and obtain 86 liters of crude extract after filtering. The crude extract was concentrated to 8.6 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 7 liters of a concentrated solution. The concentrated solution was passed through a Sephadex LH-20 resin column and eluted with 30% ethanol. The collected eluate passed through Sephadex LH-20 resin column again, and was eluted with 20% ethanol. A total of 21 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to make 120 grams of apple flavonoid powder. The total flavonoid content was 86.5% as detected by ultraviolet spectrophotometry.

[0024] The activity of scavenging · OH was measured for the apple fl...

Embodiment 2

[0029] Take 10 kg of apple pomace, place it in a multi-functional extraction tank, add 80 liters of ethanol solution with a mass concentration of 95%, heat it to 40° C., stir and extract for 4 hours, and obtain 74 liters of crude extract after filtering. The crude extract was concentrated to 14.8 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 12 liters of a concentrated solution. The concentrated solution was passed through a Sephadex LH-20 resin column and eluted with 70% ethanol. The collected eluate passed through Sephadex LH-20 resin column again, and was eluted with 60% ethanol. A total of 23 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to make 160 grams of apple flavonoid powder. The total flavonoid content was 88.4% as detected by ultraviolet spectrophotometry.

[0030] The apple flavonoids made by the present embodiment have meas...

Embodiment 3

[0035] Take 10 kg of apple pomace, put it in a multi-functional extraction tank, add 50 liters of ethanol solution with a mass concentration of 60%, heat it to 70° C., stir and extract for 2 hours, and obtain 48 liters of crude extract after filtering. The crude extract was concentrated to 6 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 5.5 liters of a concentrated solution. The concentrated solution was passed through a Sephadex LH-20 resin column and eluted with 50% ethanol. The collected eluate passed through Sephadex LH-20 resin column again, and was eluted with 40% ethanol. A total of 22 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to produce 135 grams of apple flavonoid powder. The total flavonoid content was 87.2% as detected by ultraviolet spectrophotometry.

[0036]The activity of removing RO· was measured for the apple flavono...

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Abstract

A method for extracting and purifying flavone powder from apples belongs to the field of chemical engineering technique. The method comprises selecting apple dregs, placing in a multifunctional extract tank, adding 5-10 times of ethanol solution, heating, stirring for extracting, filtering to obtain coarse extract, concentrating with a rotatory evaporator to 1 / 51 / 10 the volume of the original volume, standing for cooling, centrifuging to remove precipitate and obtain concentrate, loading on dextran gel LH-20 resin column, eluting with ethanol, collecting eluant, loading on dextran gel LH-20 resin column, eluting with ethanol, collecting eluant, concentrating with rotatory evaporator, centrifugal spray drying, and preparing into apple flavone powder. The addition amount of the flavone powder in cosmetics is 0.02%-0.5%.The invention has simple technology and extraction yield of over 1%. the purity of the extracted apple flavone can reach over 85%. The flavone can be used in cosmetics field.

Description

technical field [0001] The invention relates to a method in the technical field of chemical engineering, in particular to an extraction and purification method of apple flavonoid powder and its application. Background technique [0002] Flavonoids are a class of secondary metabolites that widely exist in fruits and vegetables. Recently, it has been found that they have a strong ability to scavenge free radicals. They are the source of important antioxidant substances in apples, and mainly exist in apple peel and pulp. The flavonoid components of apple peel mainly include rutin, quercetin, phlorizin, and flavan-3 alcohol structures such as catechin and anthocyanin. At present, it has been reported that apple flavonoids can be used as natural antioxidants in food and medicine. Although it has not been successfully applied in the field of cosmetics, it has a strong antioxidant capacity due to the large amount of hydrogen donors and the ability to form stable free radicals. Act...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K36/73A61Q19/08C07D311/30C07H1/08
Inventor 李新华张彦金素心刘雪梅刘惠娟
Owner 上海利盛生化有限公司