Blood serum copper, zincium and iron 5-br-padcap even phase automatic analysis method and liquid stabilizing reagent

A 5-br-padcap and serum copper technology, applied in the fields of medicine and biochemistry, can solve the problems of slow dissociation speed of serum copper, long color development time, and unsuitability for routine analysis

Inactive Publication Date: 2008-01-02
WENZHOU MEDICAL UNIV
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  • Abstract
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Problems solved by technology

Among them, atomic absorption spectrophotometry requires special expensive instruments and is not suitable for routine analysis; although colorimetry is accurate and simple, it has the disadvantages of cumbersome operation, slow dissociation speed of serum copper, zinc, and iron, and long color development time. It is necessary to remove serum protein, use highly toxic potassium cyanide to mask interfering ions, and then use chloral hydrate to unmasking steps. Only manual methods can be used for detection.

Method used

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  • Blood serum copper, zincium and iron 5-br-padcap even phase automatic analysis method and liquid stabilizing reagent

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Experimental program
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Embodiment 1

[0020] Embodiment 1: Serum copper two-point endpoint method assay reagent, the buffering agent is preferably acetic acid-sodium acetate buffer solution, wherein pH 3.5 acetic acid-sodium acetate buffer solution is particularly preferred in component A, and pH 5.0 is particularly preferred in component B Acetic acid-sodium acetate buffer solution; the dissociation agent is particularly preferably guanidine isocyanate; the surfactant is preferably Tween80; the stabilizer is preferably sodium chloride; the deinterfering agent is preferably a combination of hydrogen peroxide and thiourea; the preservative is preferably PC300 . In the composition of the above reagents, serum copper can be quickly dissociated from serum protein by the joint action of pH3.5 acetic acid-sodium acetate buffer solution and guanidine isocyanate. Tween80 and sodium chloride can quickly dissociate serum protein in serum copper. Maintain sufficient stability during the separation process to avoid abnormal i...

Embodiment 2

[0038] Embodiment 2: Serum iron two-point endpoint method assay reagent, the buffer solution is preferably acetic acid-sodium acetate buffer solution, wherein pH4.0 acetic acid-sodium acetate buffer solution is particularly preferred in component A, and pH7.0 is particularly preferred in component B Acetic acid-sodium acetate buffer solution; the dissociation agent is particularly preferably guanidine hydrochloride; the surfactant is preferably Tween80, the stabilizer is preferably sodium chloride, the deinterfering agent is preferably a combination of ascorbic acid and sodium fluoride, and the preservative is preferably PC800. Among the above reagent compositions, the composition used for rapid dissociation of serum iron is pH4.0 acetic acid-sodium acetate buffer, guanidine hydrochloride; the composition used to maintain the stability of serum protein during the dissociation process is Tween80 and sodium chloride.

[0039] Component A:

[0040] PH4.0 acetic acid-sodium acetat...

Embodiment 3

[0056] Embodiment 3: serum zinc two-point endpoint method assay reagent, buffer solution is preferably PH8.0 Tris-HCl damping fluid: dissociation agent is particularly preferably urea; Surfactant is preferably TritonX-100, and stabilizing agent is preferably ethylene glycol, The anti-interference agent is preferably a combination of copper reagent and trisodium citrate, and the preservative is preferably sodium azide. In the above reagent composition, the composition used for rapid dissociation of serum zinc is PH8.0 Tris-HCl buffer, urea; the composition used to maintain the stability of serum protein in the dissociation process is TritonX-100, ethylene glycol .

[0057] Component A:

[0058] PH8.0 Tris-HCl buffer 10mmol / L

[0059] Dissociating agent urea 80mmol / L

[0060] Surfactant TritonX-100 10ml / L

[0061] Stabilizer ethylene glycol 50g / L

[0062] Preservative sodium azide 0.9g / L

[0063] Anti-interfering agent copper reagent 12mmol / L

[0064] Trisodium citrate 18...

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Abstract

The invention relates to 5-Br-PADCAP even phase automatic analysis method and liquid stabilizer of serum copper, zinc, iron; it can be widely used in medicine and biochemistry technique filed, which is characterized in that: by serum copper, zinc, iron and quick decomposition of conjugated protein and application of hematocyanin stabilization technology with protein dissociation agent, the copper, zinc, iron in serum is cleavaged from the conjugated protein quickly on condition of even phase, and chromogenic reaction is happened with water soluble pyridylazo dye 5-Br-PADCAP under condition that surface activator, content measurement of copper, zinc, iron in sample can be realized on automatic analyzer in measurement mode of two-point end method. The liquid measurement agent with the method is stable, convenient, and capability of anti-interference is strong, without pollution to tube of automatic analyzer, it fits for batch automatic detection of copper, zinc, iron especially.

Description

technical field [0001] The invention relates to a 5-Br-PADCAP homogeneous automatic analysis method for serum copper, zinc and iron and a liquid type latent reagent, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] Copper, zinc, and iron are trace elements necessary for the human body, and they have many physiological functions in the human body. Numerous clinical data show that the occurrence of certain diseases is closely related to the content of copper, zinc, and iron in the human body. For example, elevated serum copper can be seen in hyperthyroidism, leukemia, Hodgkin's disease, hemochromatosis, and biliary cirrhosis , typhoid fever, tuberculosis, pernicious anemia, rheumatism and malignant tumors and other diseases, decreased serum copper can be seen in infant anemia, infant malnutrition, hepatolenticular degeneration and other diseases. Elevated serum zinc is common in acute zinc poisoning caused by industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/78
Inventor 连国军
Owner WENZHOU MEDICAL UNIV
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