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Application of lactoferrin in preparing medicament for preventing and controlling disease caused by human rotavirus

A lactoferrin, rotavirus technology, applied in the direction of antiviral agent, drug combination, digestive system, etc., can solve the problem of failure to report rotavirus inhibition or resistance, and achieve good control effect, low cost, source of wide range of effects

Inactive Publication Date: 2008-02-27
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no report has been reported to show that LF has an inhibitory or resistant effect on rotavirus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Anti-human rotavirus effect of lactoferrin

[0015] Materials and Methods:

[0016] 1. Cells: MA-104 cells were used to culture in EagleMEM medium nutrient solution (0.05% glutamine, 10% newborn bovine serum, double antibody) to form a monolayer with good shape;

[0017] 2. Virus: the Wa strain of human rotavirus is used, which is a preserved strain of the Microbiology Laboratory of Zhejiang University School of Medicine;

[0018] 3. Drug: lactoferrin: provided by Zhejiang Academy of Traditional Chinese Medicine, diluted to the experimental concentration with EagleMEM medium;

[0019] 4. Virus titration: Plaque titration;

[0020] 5. Anti-human rotavirus effect of lactoferrin: Treat virus and cells with different concentrations of lactoferrin and add maintenance solution respectively, that is, direct anti-virus, improving cell anti-virus ability and inhibiting virus proliferation. Three action links, 100 empty Plaque-forming units of human rotavirus wer...

Embodiment 2

[0029] Embodiment 2: the therapeutic effect of bovine lactoferrin (bLf) on the diarrhea of ​​rotavirus infection

[0030] In order to understand the therapeutic effect of bovine lactoferrin (bLf) on rotavirus-infected diarrhea, a model of tree shrews infected with rotavirus was established, and different doses of bLf were used to treat diarrhea tree shrews. At the same time, calculate the antigen detection rate and antigen titer of rotavirus, animal mortality, average death day, and life extension rate.

[0031] 1. Materials

[0032] 1.1 Experimental animals: adult tree shrews (healthy without diarrhea), half male and half male, provided by the Animal Center of Kunming College of Traditional Chinese Medicine;

[0033] 1.2 bLf, provided by Zhejiang Academy of Traditional Chinese Medicine;

[0034] 1.3 human rotavirus feces were collected from Kunming Children's Hospital, and the (RV-Ag) test was strongly positive for rotavirus

[0035] 1.4 Treatment of rotavirus: Collect 600...

Embodiment 3

[0060] Embodiment 3: preparation of dispersible tablets

[0061] 50g of lactoferrin, 30g of microcrystalline cellulose, 10g of mannitol, 10g of PVPP, and an appropriate amount of PVP (about 0.1g), made into 200 tablets.

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PUM

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Abstract

The invention discloses an application of lactoferrin in the drug to prevent human rotavirus, which is characterized by the following: the medium effective dose of lactoferrin is 278. 45+-12. 88mu g / mL; the medical treating index is 17. 98; the resource is wide and the cost is inexpensive, which can prevent HRV, therefore paving basis for new drug sieving.

Description

(1) Technical field [0001] The invention relates to the application of lactoferrin in the preparation of medicines for preventing and treating diseases caused by human rotavirus. (2) Background technology [0002] Lactoferrin (Lactoferrin, LF) was first isolated from milk by Groves in 1960. It is red because it combines with iron, so it is called "globin". At the beginning of its discovery, LF was considered to be a protein related to iron transport and storage, so it was also called lactotransferrin. Further studies have shown that LF is a glycoprotein with a molecular weight of 70-80kD, which is widely present in exocrine fluids such as milk, saliva, and tears, or plasma, and neutrophils. The content of LF in milk, especially colostrum is very high, for example, the mass concentration of LF in bovine colostrum is 1g / L. In-depth research has found that LF is a protein with multiple biological functions. It not only participates in the transport of iron, but also has antib...

Claims

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Application Information

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IPC IPC(8): A61K39/40A61P31/14A61P1/12
Inventor 朱婉萍孔繁智黄飞华陈小囡
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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