Micro-fluidic chip and its preparation and uses
A microfluidic chip and chip technology, applied in chemical instruments and methods, material testing products, solid adsorbent liquid separation, etc., can solve the problem of limited polymerization reaction system, difficult positioning of the monolithic column for thermally induced polymerization, and organic solvent resistance. Problems such as poor sex, to achieve the effect of a simple production method
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0024] Example 1
[0025] Such as figure 1 As shown, a microfluidic chip is formed by sealing the upper and lower substrates. The upper substrate is provided with a sample inlet 1, a sample outlet 2 and a microchannel. The microchannel on the upper substrate There is a particle introduction channel and its inlet 3, and a dam structure is provided between the particle introduction channel entrance 3 and the injection port 1 and the sample outlet 2. The two dam structures form a double cofferdam structure 4, two The microchannels between the two dam structures form a packed column bed channel 5; the positions of the lower substrate corresponding to the dam structure are respectively provided with microchannels, and there are gaps between the top surface of the dam structure and the microchannels of the lower substrate; The depth of the gap is smaller than the depth of the microchannel in the upper substrate.
[0026] Fabrication of microfluidic chip SPE column (take the fabrication...
Example Embodiment
[0034] Example 2 Chip SPE column filled with unmodified silica gel (diameter 5μm) is used for the extraction of λ-DNA
[0035] The loading, washing and elution buffers used in this example are 6M guanidine hydrochloride (dissolved in 10mM Tris-1mM EDTA buffer, pH 6.1), 80% isopropanol in water and TE buffer (10mM Tris-1mM EDTA). , PH 7.5); The sample used for extraction is λ-DNA dissolved in the loading buffer (final concentration is 0.5ng / μL). The self-made chip interface is used to connect the SPE chip to the syringe pump, and the flow rate is controlled to 3μL / min; the volumes of the sample, washing and elution solutions used are 12μL, 15μL and 15μL respectively. The entire chip extraction process takes less than 15 minutes. The eluted DNA samples were collected and quantified by fluorescence spectrophotometry with embedded fluorescent dyes to determine the extraction efficiency and repeatability of the chip SPE method.
[0036] From the experimental results (such as Figure 7 ...
Example Embodiment
[0037] Example 3 Silica-filled chip SPE column is used to purify genomic DNA from actual samples
[0038] The sample used in this example is a human whole blood sample. The processing method is: 4.6μL whole blood sample is dissolved in 500μL loading buffer (6M guanidine hydrochloride, 10mM Tris-1mM EDTA buffer, pH 6.1), and then 10μL proteinase K is added (20mg / mL), and then keep in a water bath at 56°C for 10 minutes to obtain a sample solution for extraction. The volumes of the loading, washing and elution solutions used were 15 μL, 20 μL and 15 μL, and the flow rate was 3 μL / min. The eluted DNA samples were collected every 5 μL, and 2.5 μL each was used for PCR amplification. The primer sequence used is:
[0039] 5’-AACTGTTGCTTTATAGGATTTT-3’ (justice chain),
[0040] 5'-AGGAGCTTATTGATAACTCAGAC-3' (antisense strand);
[0041] The amplified product is a 650bp fragment of the human genome β-globin gene. The composition of the PCR reaction solution (25μL) is: 10×PCR buffer 2.5μL, d...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap