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Method for producing high-butanol acetone-butanol by biological process

A technology of acetone butanol and production method, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low proportion and backward technical level, and achieve lower production costs, lower steam consumption, The effect of reducing COD content

Active Publication Date: 2008-07-02
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical level of the traditional fermentation method to produce acetone and butanol is relatively backward, and the solvent content in the fermentation broth is 18-20g / L; the proportion of butanol in the solvent is low, about 60%
This makes the cost of butanol produced by fermentation method higher than that of chemical method, but the development of modern biotechnology makes it possible to reduce the cost of butanol produced by fermentation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 10L fermenter fermentation (corn flour liquefaction)

[0037] Take 1 glycerol tube strain of Clostridium acetobutylicum (Clostridium acetobutylicum or Clostridium saccharobutylacetonicum), and inoculate it in a test tube containing 25 mL medium (30% potato, 5% sucrose, 1.0% potassium dihydrogen phosphate, natural pH), 100°C, heat shock for 1.5-2min, 38-40°C, and culture in a constant temperature incubator for 24-48 hours to obtain the first-grade seed liquid. Inoculate 25ml of first-grade seed liquid into a 10L fermenter for fermentation. The fermenter has a liquid volume of 6L. The composition of the fermentation medium is 8% corn flour and the pH is natural. Before inoculation, raise the temperature of 8% corn flour liquid to 80-100°C, add high-temperature α-amylase according to 1-30IU enzyme / g starch, liquefy for 1-50min, control the DE value of the liquefied liquid at 1-20%, and then At 121-123°C, high-temperature steam sterilization for 20-30 minutes, ...

Embodiment 2

[0039] Example 2 10L fermenter fermentation (corn starch liquefaction)

[0040] Take 1 glycerol tube strain of Clostridium acetobutylicum (Clostridium acetobutylicum or Clostridium saccharobutylacetonicum), and inoculate it in a test tube containing 25 mL medium (30% potato, 5% sucrose, 1.0% potassium dihydrogen phosphate, natural pH), 100°C, heat shock for 1.5-2min, 38-40°C, culture in a constant temperature incubator for 24-48 hours to obtain the first-grade seed liquid, inoculate 25ml of the first-grade seed liquid into a 10L fermenter for fermentation, the amount of liquid in the fermenter 6L, composition of fermentation medium: 6.5% corn starch, natural pH. Before inoculation, raise the temperature of 6.5% cornstarch solution to 80-100°C, add high-temperature α-amylase according to 1-30IU enzyme / g starch, liquefy for 1-50min, control the DE value of the liquefied solution at 1-20%, and then Add 1.0% yeast extract to the liquefaction solution. According to the nitrogen...

Embodiment 3

[0042] Example 3 10L fermenter fermentation (using potatoes as raw material for liquefaction and fermentation)

[0043] According to the method described in Example 2, obtain 25ml seed liquid, inoculate 25ml primary seed liquid in 10L fermenter and carry out fermentation, fermenter liquid volume 6L, fermentation medium composition: preparation total sugar is 6.5% potato culture Base, pH natural. Before inoculation, heat the potato liquid to 80-100°C, add high-temperature α-amylase at 1-30IU enzyme / g starch, liquefy for 1-50min, control the DE value of the liquefied liquid at 1-20%, and then add it to the liquefied liquid 1.0% yeast extract, according to the nitrogen content of the yeast extract, the carbon-nitrogen ratio is about 40-200, and the mixed material is sterilized by high-temperature steam at 121-123°C for 20-30min, and cooled to 38-40°C , inoculated and fermented. The fermentation temperature is 38-40°C, the fermentation period is 50-65 hours, the solvent outpu...

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PUM

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Abstract

The invention produces acetone and butanol using starch-containing matters, such as starch-containing subsidiary agricultural products as raw materials according to the characteristics of acetone-producing bacteria and butanol-producing bacteria. The method has butanol yield of 78%, reduces production cost, and is suitable for large-scale industrial production. The inventive method is characterized in that the method comprises the following steps of: (1) treating starch-containing matters before fermentation with enzyme method; (2) optimizing carbon-nitrogen ratio between enzymatic treatment of starch-containing matters and fermentation; and (3) increasing waste mash recycling ratio, reducing COD content in waste mash, and reducing sewage treatment capacity. The yield of butanol produced by the method is 78%. The method remarkably reduces vapor consumption, reduces COD content in waste mash, increases waste mash recycling ratio, and reduces sewage treatment capacity. And the method uses starch as raw material. The inventive method reduces production cost, and is suitable for large-scale industrial production.

Description

technical field [0001] The present invention relates to the production method of acetone and butanol. Specifically, it uses starch-containing substances, especially starchy agricultural and sideline products as raw materials, especially starch as raw material to carry out high-temperature amylase liquefaction, and then biologically produces acetone and butanol. Alcohol method. Background technique [0002] Acetone, butanol, and ethanol are collectively referred to as the total solvent of acetone, and they are important chemical material monomers for medicine, pesticides, plastics, paints, national defense, and light industries. Butanol is widely used in the production of various plastic and rubber products. In addition, butanol as a biofuel to replace gasoline has also received great attention internationally. Compared with ethanol, butanol can be blended with gasoline in higher proportions, making it more suitable for use in existing fuel supply and distribution systems. ...

Claims

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Application Information

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IPC IPC(8): C12P7/28C12R1/145
Inventor 李乃强邱勇隽廖锦绣赵宏程
Owner CATHAY R&D CENT CO LTD
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