The invention relates to the field of cigarette processing, in particular to a mixed paper-making reconstituted tobacco, as well as a preparation method and an application thereof. The preparation method comprises the concrete steps as follows: soaking mixed tobacco materials, beating, and carrying out solid-liquid separation to obtain a coating stock solution and a solid matter; adding a flavor precursor with the function of releasing the sun-cured tobacco smell and a flavor enhancement and supplementation solution into the coating stock solution to obtain a coating solution; beating the solid matter, adding wood pulp, mixing and beating to obtain a finished pulp solution, diluting the finished pulp solution, and applying a net to make a sheet base; and coating the sheet base with the coating solution, then drying and cutting, and obtaining the finished mixed reconstituted tobacco. By studying the mixed tobacco materials, the pulping process and the flavor enhancement and supplementation technology, the mixed reconstituted tobacco for cigarettes is provided, the adaptability of the reconstituted tobacco to the mixed cigarettes is improved, and the technical carrier function of the reconstituted tobacco is brought into full play.