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Hypericum perforatum total flavones and formulation preparation and quality testing method

A quality inspection method, the technology of Hypericum perforatum, is applied to medical formulas, medical preparations containing active ingredients, plant raw materials, etc., and can solve problems such as tremors or epileptic seizures, leukopenia, and slow efficacy

Active Publication Date: 2008-12-17
JIANMIN PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of medicine can uplift the mood, has a sedative effect, and has a certain therapeutic effect on insomnia symptoms, but the effect is slow and the side effects are obvious, such as dry mouth, constipation, blurred vision, difficulty urinating, urinary retention, and a few tremors may occur Or epilepsy, etc.; heterocyclic antidepressants also have a certain therapeutic effect on depression, but there are side effects such as leukopenia, dizziness, blurred vision, drowsiness, constipation, weight gain, etc. On the other hand, rashes are also more common. Although less toxic to the heart, occasionally has defects such as seizures

Method used

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  • Hypericum perforatum total flavones and formulation preparation and quality testing method
  • Hypericum perforatum total flavones and formulation preparation and quality testing method
  • Hypericum perforatum total flavones and formulation preparation and quality testing method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0239] Hypericum perforatum total flavonoids granules were prepared by conventional methods, and HPLC fingerprint detection was carried out:

[0240] Chromatographic conditions: Hanbang C 18 Chromatographic column Kromasil, 4.6mm×254mm, 5μm; Dikma pre-protected column; detection wavelength: 254nm; column temperature: 25°C; acetonitrile as mobile phase A, 0.5% phosphoric acid solution as mobile phase B, the sum of the concentrations of A and B 100%, carry out gradient elution; 0~30min, the ratio of A-B is 15:85; 30~60min, the ratio of A-B changes linearly from 15:85 to 40:60; 60~80min, the ratio of A-B changes from 40:60 Change linearly to 100:0; 80-85min, the ratio of A-B is linearly changed from 100:0 to 15:85; 85-100min, the ratio of A-B is 15:85; injection volume: 10μl;

[0241]Preparation of reference substance solution: Preparation of single-component reference substance solution: precisely measure the reference substance dried by phosphorus pentoxide for 48 hours, disso...

Embodiment 2

[0244] After crushing 1 kg of Hypericum perforatum whole herb, extract it with 5 kg of 80% ethanol, put it in an extraction tank, extract once under reflux, and filter to obtain the extract: control the temperature of the extract to be below 50°C, concentrate it, and spray it to a specific gravity of 1.15 Dry to obtain the extract: the extract is degreased with 3kg of n-hexane to remove the fat-soluble components to obtain the degreased extract: the degreased extract is extracted with ethyl acetate to obtain the precipitate and ethyl acetate extract, and the ethyl acetate The lipid extract was concentrated to a specific gravity of 1.32 to obtain the ethyl acetate part mainly containing hypericin and quercetin, i.e. part A: the precipitate part was dissolved in water, and the excess organic solvent was volatilized at the same time to obtain the aqueous solution part; After the aqueous solution part is fully cooled, after centrifugation, apply polystyrene weakly polar macroporous...

Embodiment 3

[0248] After cutting 1kg of Hypericum perforatum into sections, extract it with 8kg of 65% ethanol, put it in an extraction tank, extract twice under reflux, and filter to obtain the extract: control the temperature of the extract below 60°C and concentrate it to a specific gravity of 1.12 Spray drying to obtain the extract: degrease the extract with 6kg of petroleum ether to remove the fat-soluble components to obtain the degreased extract: extract the degreased extract with ethyl acetate to obtain the precipitate and ethyl acetate extract. Concentrate the ethyl acetate extract to a specific gravity of 1.25 to obtain the ethyl acetate part mainly containing hypericin and quercetin, that is, part A: dissolve the precipitated part in water, and volatilize the excess organic solvent at the same time to obtain the aqueous solution part; After fully cooling the aqueous solution, apply polystyrene medium-large-porous resin at a flow rate of 4 times the column volume: elute with 5 ti...

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Abstract

The invention discloses a total flavonoid from hypericum perforatum, a preparation method of a preparation thereof and a quality test method thereof. The preparation method of the invention mainly adopts the steps of degreasing, extracting ethyl acetate, and the like, which has the advantages of less loss of heat sensitivity components, stable content of effective component and better guarantee for product yield and quality compared with the prior art. The quality test method of the invention can successfully control the quality of the product and ensure the relevant efficacy of the product. The total flavonoid from hypericum perforatum and the preparation thereof have better efficacy on treating depression.

Description

technical field [0001] The invention relates to a preparation method and a quality detection method of total flavonoids of Hypericum perforatum and its preparation, in particular to a preparation method and quality detection method of antidepressant Hypericum perforatum total flavonoids and its preparation. Background technique [0002] Depression, the main type of affective disorder, is a syndrome characterized by significant and persistent low mood. With the acceleration of the modern industrialization process and the fast-paced and spiritual and psychological pressure brought by the market economy, the incidence of mental depression has increased significantly. The world's large-scale epidemiological study shows that depression currently ranks fourth among disabling diseases in the world, and will rank second by 2020, second only to cardiovascular diseases. Scientific research has confirmed that both psychological and non-psychological factors can induce depression, and ...

Claims

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Application Information

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IPC IPC(8): A61K36/634A61P25/24C07D311/30
Inventor 黄志军李崇明曾庆恢朱立彬袁铭
Owner JIANMIN PHARMA GRP CO LTD
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