Synthesis process with amide zymoid method for pressure reduction azeotropy continuous water elimination

Abstract

The invention belongs to the field of nonaqueous phase enzymatic organic synthesis application biotechnology, and in particular relates to a process of amide-type enzymatic synthesis by decompressed azeotropic continuous dehydration. The process is generally suitable for synthesis of various amido bonds catalyzed by biocatalyst (such as neutral protease). The invention provides a generally practicable novel process for amide-type enzymatic synthesis; and under the premise of not affecting catalytic activity of the biocatalyst, through decompressed azeotropic continuous dehydration, reaction balance moves to the synthetic direction of amide, so as to improve conversion rate of enzymatic organic synthesis greatly, ensure that the reaction tends to finish, solve the continuous water-removing problem in the nonaqueous phase enzymatic organic synthesis thoroughly, improve purity and yield of products, reduce production cost, and have wide application prospect in the fields such as medicine, food, material, fine chemical industry and the like.

Description

technical field [0001] The invention belongs to the field of applied biotechnology of non-aqueous phase enzymatic organic synthesis, in particular to an amide enzymatic synthesis process of decompression azeotropic continuous water removal. Background technique [0002] Regarding the synthesis of amides, there are two methods of chemical synthesis and biocatalytic synthesis. The chemical synthesis process is very mature, and is still widely used in the industrial production of amides organic compounds. But there are some fatal flaws in the chemical synthesis process. This process often requires the use of strong liquid acids such as concentrated sulfuric acid or p-methoxybenzenesulfonic acid as a catalyst, and the reaction temperature is very high, usually accompanied by unavoidable side reactions, which have a great impact on the conversion rate of raw materials and the yield of products. Large, the separation and purification process is complicated, and it also has extre...

AI Technical Summary

Problems solved by technology

Molecular sieve adsorption method is convenient and easy to implement, with obvious effect, and is often used in laboratory research. However, since the regeneration of molecular sieve after adsorption saturation requires special equipment and technology, the investment cost is high, so it cannot be applied in large-scale industrial production.

Membrane separation and water absorption method is theoretically feasible, but it is still difficult to realize due to problems such as membrane ducer, aging and service life.

Use tert-amyl alcohol, tert-butanol, etc., which can be compatible with enzymes to ensure the catalytic activity of enzymes, and can form an azeotropic mixture with the generated water as the reaction medium and water agent, and use negative pressure azeotropic distillation It is a good choice to take the water away by means of water, but unfortunately, the formed azeotropic mixture does not separate after condensation, so the continuous separation of water and the continuous recycling of solvent cannot be realized. The distilled water-containing solvent must be After special drying treatment, it can be reused

Drying costs of aqueous solvents greatly limit the application of this technology