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Micro-ecological formulation and preparation method thereof

A technology of micro-ecological preparations and strains, applied in the field of treatment and recycling of vitamin fermentation industrial wastewater, can solve problems such as high energy consumption and equipment corrosion, achieve the effects of degrading pesticide residues, solving pollution problems, and saving pollution control costs

Inactive Publication Date: 2009-08-19
马兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is reported that at present, some domestic manufacturers adopt the method of evaporation for treatment, which consumes a lot of energy, and at the same time, the equipment is seriously corroded. The enterprise can no longer bear the heavy burden of wastewater treatment for a long time.
So far, domestic research on this aspect has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture Embodiment 1

[0017] Manufacture Example 1: Treat 100 kilograms of vitamin B12 waste water:

[0018] 1. Strain preparation: Saccharomyes cerevisiae Meyen exHansen CGMCC AS 2.121, Lactobacillus fermentum AS 1.122 and Bacillus cereus CGMCC AS 1.895 were used for compound culture.

[0019] Preparation of seed solution: medium: 2.5 grams of brown sugar, 0.5 grams of peptone, dilute to 100 ml with vitamin B12 waste water, sterilize at 115°C for 30 minutes; add wort activated yeast and nutrient broth activated yeast after cooling down Lactic acid bacteria and bacillus were cultured at a controlled temperature of 37±1°C for 48 hours to obtain a compound seed solution;

[0020] 2. Fermentation: Put 100 kg of vitamin B12 waste water into the fermentation device, add 300 g of glucose and 3000 g of peptone, sterilize and cool down, insert 100 ml of compound seed liquid, stir well, and control the temperature at 37±1°C for cultivation. During the cultivation process , stirring slowly intermittently, c...

manufacture Embodiment 2

[0021] Manufacture Example 2: Treat 100 kilograms of vitamin B12 waste water;

[0022] 1. Strain preparation: the strains were Hansenuse anomala CGMCC2.296, Lactobacillus delbrueckii subsp bulgaricus CGMCC AS 1.1482, Bacillus subtilis CGMCC AS1.140 Compound culture of three kinds of bacteria;

[0023] (1), compound culture of yeast and lactic acid bacteria:

[0024] Preparation of primary seeds: the culture medium is: 2 grams of glucose, 20 grams of beef extract, dilute to 2000 ml with vitamin B12 waste water, sterilize at 115°C for 30 minutes; The activated bacterial classification of Lactobacillus subsp. bulgaricus and Bacillus subtilis is the same as in Example 1, and the temperature is controlled at 34 ± 1° C. for 24 hours to obtain a composite seed liquid;

[0025] 2. Fermentation: Put 100 kg of vitamin B12 waste water into the fermentation device, add 5000 grams of molasses, 1000 grams of beef extract, sterilize and cool down, insert 2000 ml of compound seed liquid, st...

manufacture Embodiment 3

[0026] Manufacture Example 3: Treat 100 kilograms of vitamin B12 waste water:

[0027] 1. Strain preparation: Saccharomyes cerevisiae Meyenex Hansen CGMCC 2.1416, L.acidophilus CGMCC 1.1854 and Bacillus licheniformis CGMCC AS 1.932 were used for compound culture.

[0028] Preparation of liquid seeds: the culture medium is: 500 grams of molasses, 5 grams of yeast extract, dilute to 5000 milliliters with vitamin B12 waste water, sterilize at 115 ° C for 30 minutes; insert the activated above-mentioned strains after cooling down, the activation method is the same as the implementation Example 1, control the temperature at 30±1°C and cultivate for 72 hours to obtain a compound seed liquid;

[0029] 2. Fermentation: Put 100 kg of vitamin B12 wastewater into the fermentation device, add 5,000 grams of brown sugar, 300 grams of yeast extract, add 5,000 liters of compound seed liquid, stir evenly, and control the temperature at 30±1°C for cultivation. During the cultivation process, i...

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PUM

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Abstract

The invention relates to the technical field of recycling waste water produced by fermenting vitamin B12, and discloses a microbial ecological agent and a preparation method thereof. The invention more particularly relates to a microbial ecological agent product prepared by taking the waste water produced by fermenting vitamin B12 as raw material through the fermentation process of lactobacillus, microzyme and bacillus. Furthermore, the product is applied for nuisanceless agriculture; the invention develops the usage of the waste water of vitamin B12, so as to change waste into valuable and realize zero emission in the process of producing waste water by fermenting vitamin B12.

Description

Technical field: [0001] The invention relates to the technical field of treatment and recycling of vitamin fermentation industrial wastewater, in particular to the regeneration and utilization of vitamin B12 fermentation wastewater. Specifically, it uses vitamin B12 waste water as a raw material, and is prepared into a microecological preparation product through a microbial fermentation process; the invention also relates to the application of the microecological preparation in the field of breeding and planting. Background technique: [0002] Fermentative production of vitamin B12 is prepared by using propionibacterium, rhodopseudomonas, etc. as strains, and molasses as the main raw material, through liquid submerged fermentation and separation and extraction processes. During the separation and extraction process, a large amount of high-concentration wastewater is produced. The wastewater contains high ammonia nitrogen and inorganic salts, etc., and the COD reaches 10,000 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P1/04C02F3/34C05F11/08C12R1/225C12R1/865C12R1/645C12R1/07C02F103/32
Inventor 马兰孙玉胜
Owner 马兰
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