Fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Toxoplasma gondii

A fluorescence quantification and Toxoplasma gondii technology, which is applied in the direction of fluorescence/phosphorescence, microbe-based methods, microbiological measurement/inspection, etc., can solve the problems of long culture time, PCR pollution, and limited diagnostic value, etc. The effect of good repeatability and fast detection speed
CN101613751AInactive Publication Date: 2009-12-30MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2009-12-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescent quantitative PCR (FQ-PCR) kit for rapidly detecting Toxoplasma gondii, relating to gene detection technology of zoonosis pathogen, and being applicable to qualitative and quantitative detection of Toxoplasma gondii. The invention comprises DNA extracting solution, a standard positive template, a fluorescent quantitative PCR reaction solution, Toxoplasma gondii B1 gene specific primer and a negative quality control standard product. The invention is accurate in quantifying, rapid in detection speed, good in specificity, high in sensitivity, simple in use steps and high in repeatability and can replace traditional etiology detection method.
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Description

technical field

[0001] The invention belongs to the technical field of detecting pathogens of zoonotic infectious diseases, in particular to a fluorescent quantitative PCR kit for rapid detection of toxoplasma gondii, which is suitable for qualitative and quantitative detection of toxoplasma gondii. Background technique

[0002] Toxoplasma gondii is a parasitic protozoan widely parasitic in the nucleated cells of humans and animals, with cats as its final hosts and humans and many vertebrates as intermediate hosts. The worm is distributed worldwide and can infect humans and many animals, causing zoonotic toxoplasmosis. Due to its great potential harm to human health and agricultural and animal husbandry production, it is very important to diagnose toxoplasmosis quickly and accurately.

[0003] In terms of clinical detection, the traditional etiological detection method has the disadvantages of low detection rate and long incubation time. The traditional serological method i...

Claims

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