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A method and special medium for preparing schizophyllan

A technology of schizophyllan and culture medium, which is applied in the field of preparation of schizophyllan, can solve the problems of underutilization and waste of resources, and achieve the effects of reducing raw material costs, high economic value, and anti-tumor activity

Inactive Publication Date: 2012-02-01
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, these resources have not been fully utilized, and resources are seriously wasted.

Method used

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  • A method and special medium for preparing schizophyllan
  • A method and special medium for preparing schizophyllan
  • A method and special medium for preparing schizophyllan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, schizophyllan and preparation method thereof

[0034] 1. Preparation method

[0035] (1) Activation of bacteria

[0036] Inoculate Schizophyllum commune Fr. CFCC NO.6812 into PDA slant medium for rejuvenation and activation culture.

[0037] The composition of the PDA slant medium is as follows: every liter of the medium contains 20 g of potatoes, 2 g of glucose, 0.5 g of yeast extract, 0.15 g of magnesium sulfate, 0.3 g of potassium hydrogen phosphate and 2 g of agar, and the rest is water.

[0038] (2) Expanded culture of bacteria

[0039] Take the cultured slant strains, inoculate them into liquid PDA medium, and cultivate them for 48-52 hours at 25-29°C with a shaker speed of 150-180 rpm to obtain evenly distributed mycelium balls and clear and transparent fermentation broth The liquid strain, this is a first-class liquid strain.

[0040] The first-grade liquid bacteria are inoculated into the liquid PDA medium, and cultured at 25-29° C. and the ro...

Embodiment 2

[0110] Embodiment 2, schizophyllan and preparation method thereof

[0111] 1. Preparation method

[0112] (1) Activation of bacteria

[0113] Using Schizophyllum commune Fr. CFCC No.83457 strain, the method is consistent with that described in Example 1.

[0114] (2) Expanded culture of bacteria

[0115] The method is consistent with that described in Example 1.

[0116] (3) Solid fermentation of strains

[0117] In an aseptic operating environment, the pre-fermented secondary liquid strains are inoculated into the fermentation medium, and the inoculation ratio is secondary liquid strains: fermentation medium=50mL: 1000g; placed at 26°C for 10 days, When the mycelium is covered with the bacteria bag (or bacteria bottle), the solid fermentation is finished, and the fermented mixture is used as the fermentation raw material.

[0118] The composition of the fermentation medium is as follows: each liter of medium contains 400g of corn bran, 100g of rice bran, growth-promoting...

Embodiment 3

[0146] Embodiment 3, the preparation method of schizophyllan

[0147] 1. Activation of bacteria

[0148] Schizophyllum commune Fr. (Schizophyllum commune Fr.) ACCC NO.50875 was used, and the method was consistent with that described in Example 1.

[0149] Second, the expansion of the culture of bacteria

[0150] The method is consistent with that described in Example 1.

[0151] 3. Solid fermentation of strains

[0152] In an aseptic operating environment, inoculate the pre-fermented secondary liquid strains into the fermentation medium at an inoculation ratio of secondary liquid strains: fermentation medium = 100mL: 1000g; place at 29°C for 7 days , when the mycelium is covered with bacteria bags (or bacteria bottles), the solid fermentation ends, and the fermented mixture is called fermentation raw material.

[0153] The composition of the fermentation medium is: each liter of medium contains 430g of corn stalks, 70g of bagasse, and growth-promoting factor-V B1 0.05g, L...

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Abstract

The invention discloses a method for preparing schizophyllan and a special culture medium. The composition of the culture medium is as follows: every kilogram of culture medium contains 400-450g of corn cob, corn stalk or corn bran, 50-100g of wheat bran, rice bran or bagasse, 0.05-0.1g of growth-promoting factor VB1, L-glutamic acid 0.05-0.1g, the rest is water. The method for preparing schizophyllan by solid fermentation of the present invention uses agricultural and by-product resources (corn stalks, corncobs, corn bran, wheat bran, bagasse, etc.) rich in fiber substrates as raw materials for fermentation, greatly reducing the raw material cost, and realized the efficient transformation of agricultural and sideline product resources, which is both environmentally friendly and economical; the method has a high yield of schizophyllan, and the polysaccharide content in the fermentation raw material can reach 4.5-5.8% (mass percentage content) .

Description

technical field [0001] The invention relates to a method for preparing schizophyllan and a special culture medium. Background technique [0002] Schizophyllum commune Fr., also known as white ginseng fungus and tree flower fungus, is a white saprophytic basidiomycete fungus with high edible and medicinal value. Schizophyllum is widely distributed in nature. It can effectively degrade lignin, hemicellulose and some cellulose in fiber substrates. It can grow well under relatively harsh conditions. It is an easy-to-cultivate medicinal fungus. Schizophyllan is a water-soluble exopolysaccharide present in the fruiting body, mycelium or fermentation broth of Schizophyllum fungi, with a typical β-(1,3)-D-glucan backbone of medicinal fungal polysaccharides , β-(1,6)-D-glucose branched structure. Various physiological activities of schizophyllan, such as anti-tumor, anti-bacterial and anti-inflammatory, anti-radiation, and improving immunity of the body, have been confirmed by rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 周素梅王强钟昕高利忠刘红芝陈聪
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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