Preparation method of mangiferin

A technology for mangiferin and raw materials, applied in the field of compound extraction technology, can solve the problems of high cost, cumbersome preparation process, insufficient deep processing of products, etc., and achieves the effects of mild production process conditions, reduced production cost, and improved yield.

Inactive Publication Date: 2010-03-17
HAINAN JINXING PHARMA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] At present, the research on mangiferin in China only stays in the extraction of total medicinal flavonoids. The main products are Chinese patent medicines of mango cough tablets, and the main effect is to relieve cough. At the same time, most of the extraction of mangiferin is from plants such as Zhimu. Extracted from mangiferin, and did not consider recycling from the aspect of environmental resource reuse. Although some manufacturers in China have conducted research on mango leaves, they all use the crude mangiferin extract as a chemical raw material for sales. One product has low technological content. , the second is that the deep processing of the product is not enough, the third is that the high safety of mangiferin is ignored, and the development of functional food additives is not considered, the fourth is that the preparation process is cumbersome and the cost is high, and the fifth is that the amount of organic solvent used in the purification process is relatively large

Method used

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  • Preparation method of mangiferin
  • Preparation method of mangiferin
  • Preparation method of mangiferin

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take 100g of dry leaves of Mangiferaindica L., a plant of Anacardiaceae, crush them, soak them in 800ml of cellulase aqueous solution with pH=4.5 (140μg of cellulase per g of crude drug) for 0.5hr, then enzymatically hydrolyze in a water bath at 50°C for 2hr, and stir thoroughly , filter, get the dregs of the medicine and degrease twice with 600ml of petroleum ether, filter, reclaim the petroleum ether, extract the dregs of medicine twice with 75% ethanol equivalent to 6 times the amount of the original medicinal material, combine the extracts, and reclaim the ethanol , spray-dried to obtain a dry powder, then dissolve the powder with 20% ethanol, centrifuge for 15 minutes at a speed of 6000 rpm, take the filtrate, concentrate until dry to obtain the crude mangiferin, and recrystallize twice with 40% ethanol. Finally, the fine mangiferin was obtained.

Embodiment 2

[0029] Take 100 g of dry leaves of Mangiferaindica L. of Anacardiaceae, crush them, and use 1000 ml of cellulase (140 μg of cellulase per g of crude drug) and pectinase (80 μg of pectinase per g of crude drug) at a ratio of 1:1 Soak in the aqueous solution for 0.5hr, then enzymatically hydrolyze in a water bath at 50°C for 4hr, stir well, and centrifuge for 30min at a speed of 10,000rpm, take the dregs and use 1000ml of petroleum ether to degrease twice, filter, and recover petroleum ether , the dregs are extracted three times with 75% ethanol equivalent to 10 times the amount of the original medicinal material, the extracts are combined, the ethanol is recovered, and spray-dried to obtain a dry powder, then the powder is dissolved with 20% ethanol, centrifugally filtered, and the filtrate is concentrated to The crude mangiferin was obtained by drying, and recrystallized three times with 40% ethanol to finally obtain the refined mangiferin.

Embodiment 3

[0031] Take 100 g of mango leaves, soak them in 800 ml of cellulase aqueous solution with pH=4.5 (add 140 μg of cellulase per g of crude drug) for 0.5 hr, then enzymatically hydrolyze in a water bath at 50 °C for 2 hr, stir well, filter, and take the dregs for each The sherwood oil of 600ml carries out degreasing treatment twice, filters, reclaims sherwood oil, and dregs is extracted twice with the 75% ethanol that is equivalent to 6 times of former medicinal material, merges extract, reclaims ethanol, sprays and dries to obtain dry powder, and then The powder is dissolved with 20% ethanol, filtered, the filtrate is taken, concentrated to dryness to obtain crude mangiferin, and then recrystallized twice with 40% ethanol to finally obtain fine mangiferin.

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Abstract

The invention discloses a preparation method of mangiferin, comprising the following steps: (1) taking raw material mango leaves, drying, pulverizing, using enzyme solution in an amount of 6 to 10 times the amount of the raw material by weight to soak for 0.1hr to 1.0hr, then carrying out water bath and enzymolysis in the case of the pH value ranging from 4.0-7.0 and the temperature ranging from 35 DEG C to 55 DEG C, and fully stirring; (2) filtering, taking dregs of a decoction, using petroleum ether in an amount of 6 to 10 times by weight respectively to 2 to 3 times; (3) using 60%-80% of ethanol water in an amount of 6 to 10 times of the raw material by weight to extract the degreased dregs of a decoction of step (2) 2-3 times, combining the extracting liquid, and recycling alcohol; (4)spraying and drying the extracting liquid of step(3) to obtain dry powder, using 10%-30% of ethanol water to dissolve the dry powder, and then centrifuging and filtering; and (5) taking the filter liquor after centrifugal filtration in (4), carrying out water bath and pressure reduction, concentrating and drying to obtain the mangiferin. In the invention, an enzymatic method is utilized to extract the mangiferin from mango leaves so as to utilize waste materials, thereby greatly lowering the cost.

Description

technical field [0001] The invention relates to a compound extraction process, in particular to a preparation method of mangiferin, in particular to a method for extracting mangiferin from mango leaves by a biologically active enzyme method. Background technique [0002] Mangiferin, also known as mangiferin and anemarrhena, mainly exists in the fruits, leaves, and bark of Mango (Mangifera indica L.), and the rhizome of Anemarrhena asphodeloides Bge. , the aboveground part, in the flowers and leaves of the Iridaceae plant Shegan [Belamcanda chinensis (L.) DC.]. [0003] Pharmacological functions of mangiferin: inhibiting the central nervous system, anti-inflammation, antibacterial, anti-herpes simplex virus, choleretic and immune effects, and has a protective effect on simulated plateau acute hypoxic liver injury. Pharmacological experiments show that it has good curative effect in treating chronic bronchitis, is the main active ingredient of Tibetan Yinchen in treating hepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
Inventor 展学孔伍曾利刘全胜
Owner HAINAN JINXING PHARMA
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