Preparation method of mangiferin

Abstract

The invention discloses a preparation method of mangiferin, comprising the following steps: (1) taking raw material mango leaves, drying, pulverizing, using enzyme solution in an amount of 6 to 10 times the amount of the raw material by weight to soak for 0.1hr to 1.0hr, then carrying out water bath and enzymolysis in the case of the pH value ranging from 4.0-7.0 and the temperature ranging from 35 DEG C to 55 DEG C, and fully stirring; (2) filtering, taking dregs of a decoction, using petroleum ether in an amount of 6 to 10 times by weight respectively to 2 to 3 times; (3) using 60%-80% of ethanol water in an amount of 6 to 10 times of the raw material by weight to extract the degreased dregs of a decoction of step (2) 2-3 times, combining the extracting liquid, and recycling alcohol; (4)spraying and drying the extracting liquid of step(3) to obtain dry powder, using 10%-30% of ethanol water to dissolve the dry powder, and then centrifuging and filtering; and (5) taking the filter liquor after centrifugal filtration in (4), carrying out water bath and pressure reduction, concentrating and drying to obtain the mangiferin. In the invention, an enzymatic method is utilized to extract the mangiferin from mango leaves so as to utilize waste materials, thereby greatly lowering the cost.

Description

technical field [0001] The invention relates to a compound extraction process, in particular to a preparation method of mangiferin, in particular to a method for extracting mangiferin from mango leaves by a biologically active enzyme method. Background technique [0002] Mangiferin, also known as mangiferin and anemarrhena, mainly exists in the fruits, leaves, and bark of Mango (Mangifera indica L.), and the rhizome of Anemarrhena asphodeloides Bge. , the aboveground part, in the flowers and leaves of the Iridaceae plant Shegan [Belamcanda chinensis (L.) DC.]. [0003] Pharmacological functions of mangiferin: inhibiting the central nervous system, anti-inflammation, antibacterial, anti-herpes simplex virus, choleretic and immune effects, and has a protective effect on simulated plateau acute hypoxic liver injury. Pharmacological experiments show that it has good curative effect in treating chronic bronchitis, is the main active ingredient of Tibetan Yinchen in treating hepa...

AI Technical Summary

Problems solved by technology

[0005] At present, the research on mangiferin in China only stays in the extraction of total medicinal flavonoids. The main products are Chinese patent medicines of mango cough tablets, and the main effect is to relieve cough. At the same time, most of the extraction of mangiferin is from plants such as Zhimu. Extracted from mangiferin, and did not consider recycling from the aspect of environmental resource reuse. Although some manufacturers in China have conducted research on mango leaves, they all use the crude mangiferin extract as a chemical raw material for sales. One product has low technological content. , the second is that the deep processing of the product is not enough, the third is that the high safety of mangiferin is ignored, and the development of functional food additives is not considered, the fourth is that the preparation process is cumbersome and the cost is high, and the fifth is that the amount of organic solvent used in the purification process is relatively large

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