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Human tissue engineering support loaded with controlled-release cell growth factor and provided with hollow silicon dioxide ball with kernel and preparation method and applications thereof

A technology of silica spheres and growth factors, which is applied in the field of biomedical material preparation, can solve the problems of poor mechanical strength of human tissue engineering scaffolds, lack of slow-release cell growth factors, and lack of slow-release cell growth factors. Facilitate cell adhesion and growth, save dosage, increase tropism and adhesion

Inactive Publication Date: 2010-06-09
张阳德 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, a variety of methods have been developed to prepare human tissue engineering scaffolds with various structures, but the obtained human tissue engineering scaffolds generally have poor mechanical strength and do not have the function of slow-release cell growth factors.
Invention patent "Preparation method of chitosan tubular scaffold" (publication number: CN1568904) discloses a preparation method of chitosan tubular scaffold prepared by textile method, but this scaffold does not have slow-release cell growth factor function; in the invention patent "a construction method of skin tissue engineering scaffold containing epidermal growth factor" (publication number: CN1511592), the skin tissue engineering scaffold containing epidermal growth factor was obtained by freeze-drying method, but the obtained tissue engineering scaffold strength poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1.0.001g of hollow silica submicron spheres with a core (core size 50nm) (particle size 100nm, shell thickness 22nm, mesopore diameter 3nm, specific surface area 100m 2 / g) Soak in 0.001mmol / L aqueous solution of nerve growth factor, and stir for 12 hours at a temperature of 4°C. Centrifuge at a temperature of 4° C., wash the resulting solid product three times with deionized water, and freeze-dry to obtain a hollow silica sphere with an inner core loaded with nerve growth factor.

[0036] 2. Ultrasonic disperse the obtained hollow silica spheres with inner core loaded with nerve growth factor in an aqueous gelatin solution with a mass concentration of 1%, and the final result of the hollow silica spheres with inner core loaded with nerve growth factors in aqueous gelatin solution is: Concentration is 10 -3 mg / mL, freeze-dried to obtain a composite ball composed of hollow silica submicron spheres with an inner core and gelatin loaded with nerve growth factor. The part...

Embodiment 2

[0041] 1.10g of hollow silica submicron spheres (particle size is 980nm, shell thickness is 20nm, mesopore diameter is 10nm, specific surface area is 400m2) with core (core size is 500nm) 2 / g) soaked in a 10mmol / L aqueous solution of epidermal growth factor, and stirred for 72 hours at a temperature of 4°C. Centrifuge at a temperature of 4° C., wash the resulting solid product three times with deionized water, and freeze-dry to obtain a hollow silica sphere with an inner core loaded with epidermal growth factor.

[0042] 2. Ultrasonic disperse the obtained hollow silica spheres with inner core loaded with epidermal growth factor in an aqueous gelatin solution with a mass concentration of 0.1%, and the final result of the hollow silica spheres with inner core loaded with epidermal growth factor in aqueous gelatin solution is: The concentration is 100 mg / mL, freeze-dried to obtain a composite ball composed of hollow silicon dioxide submicron balls with inner cores loaded with e...

Embodiment 3

[0047] 1.0.1g of hollow silica submicron spheres with inner core (core size 250nm) (particle size 700nm, shell thickness 50nm, mesopore diameter 20nm, specific surface area 1000m 2 / g) soaked in 0.1 mmol / L bone morphogenetic protein aqueous solution, and stirred for 48 hours at a temperature of 4°C. Centrifuge at a temperature of 4° C., wash the resulting solid product three times with deionized water, and freeze-dry to obtain a hollow silica sphere with an inner core loaded with bone morphogenetic protein.

[0048] 2. Ultrasonic disperse the obtained hollow silica spheres with inner cores loaded with bone morphogenetic proteins in an aqueous gelatin solution with a mass concentration of 9.8%, and the hollow silica spheres with inner cores loaded with bone morphogenetic proteins in aqueous gelatin solution The final concentration was 1 mg / mL, and freeze-dried to obtain a composite ball composed of hollow silica submicron balls with an inner core loaded with bone morphogenetic ...

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Abstract

The invention belongs to the technical field of bomedical material preparation, in particular relates to human tissue engineering support loaded with controlled-release cell growth factor and provided with hollow silicon dioxide ball with kernel and a preparation method and applications thereofThe human tissue engineering support is prepared in the following steps: the hollow silicon dioxide submicron ball loaded with cell growth factors and provided with kernel is self-assembled with gelatin to form a compound ball which is composed of the hollow silicon dioxide submicron ball loaded with cell growth factors and provided with kernel and the gelatin; then the compound ball is loaded to macromolecule polymer fiber base material obtained by the electric spinning method, thus obtaining the human tissue engineering support.With hollow silicon dioxide submicron ball with kernel as a slow release carrier of the cell growth factors, cell proliferation and differentiation is promoted by loading and slowly releasing the cell growth factors.The human tissue engineering support of the invention is an all-round imitation of the cell growth microenvironment, thus enjoying broad application prospect in the field of tissue engineering support covering tissue repair and tissue reconstruction.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a human tissue engineering scaffold loaded with a slow-release cell growth factor and a hollow silica sphere with an inner core, and a preparation method and application thereof. Background technique [0002] The lack of sources of human tissue, especially the major organs of the human body, has always been a major obstacle to the repair or improvement of human diseased tissue or human organ tissue transplantation; the biological activity used to repair or improve the structure and function of human diseased tissue or human organ The development of substitute human tissue engineering products will help greatly improve human health. Human tissue engineering products load the normal tissue cells cultured and expanded in vitro on a biomaterial that has excellent cytocompatibility and can be degraded and absorbed by the human body, and then the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/22A61L27/02A61L27/54A61F2/82
Inventor 张阳德陈东李琳琳唐芳琼刘惠玉孟宪伟周健张宗久
Owner 张阳德
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