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Collagen polypeptide, preparation method and application thereof

A collagen polypeptide and keratinase technology, applied in the field of enzyme catalysis, can solve problems such as incomplete hydrolysis, fishy smell, and difficulty in removal

Active Publication Date: 2010-07-28
香港百特有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, my country has generally adopted enzymatic method to produce animal hydrolyzed collagen. Although the above problems have been initially solved, and the application and development of collagen have been improved and promoted to a certain extent, there are still many problems, such as: 1. Collagen has a large molecular weight , poor solubility, unable to be directly absorbed by cells; 2. The existing collagen peptide production process is complex, high cost, and product quality is unstable; 3. The existing enzymatic production process of collagen peptides, hydrolysis is not complete, The utilization rate of raw materials is low; although the techniques of chemical and physical methods can fully hydrolyze collagen, they will destroy amino acids such as tryptophan and serine, and the obtained hydrolyzate basically has no biological activity; 4. Existing collagen The protein peptide still has a bitter and fishy smell, and it is difficult to remove; 5. The existing collagen peptide will be hydrolyzed by pepsin and trypsin after entering the human body, and most of its activity will be lost

Method used

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  • Collagen polypeptide, preparation method and application thereof
  • Collagen polypeptide, preparation method and application thereof
  • Collagen polypeptide, preparation method and application thereof

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preparation example Construction

[0067] The invention provides a preparation method of collagen polypeptide, which comprises the steps of: adding compound enzyme A to hydrolyze the raw material after pretreatment, after the hydrolyzate is subjected to microfiltration, desalting and decolorizing through ion exchange resin, and finally making collagen polypeptide .

[0068] In a preferred embodiment of the present invention, based on the total weight of solids, the compound enzyme A is added in an amount of 2-5w / w% of the solids content.

[0069] In a preferred embodiment of the present invention, the hydrolysis used is under the conditions of 50°C-60°C and pH 9-10 for 6-10 hours.

[0070] In another preferred embodiment of the present invention, the raw material of the method for preparing collagen polypeptide is animal skin, taking fish skin or pig skin as an example, comprising the following steps:

[0071] 1) Raw material pretreatment Ⅰ: cleaning and degreasing the fish skin or dehaired pig skin to remove ...

Embodiment 1

[0116] Hydrolysis of Collagen by Compound Enzyme A

[0117] In the range of 40-80w / w%, gradually adjust the percentage concentration of keratinase in the complex enzyme A, then according to step 1) to step 4) described in Example 2 (1), or Example 2 (2) Step 1) to step 3) described in, or step 1) to step 4) described in embodiment 2 (3) implements compound enzyme A to the hydrolysis of collagen (the amount of compound enzyme A is according to 3w of raw material amount / w% added), after hydrolysis finishes, get enzymolysis solution 5ml12000rpm centrifugal 5 minutes, get supernatant and measure soluble protein content with Folin-phenol method, and calculate degree of hydrolysis DH according to following formula:

[0118] DH ( % ) = ( N 2 - N 1 ) ...

Embodiment 2

[0130] Preparation of Collagen Peptides

[0131] (1) Use animal skins such as pigskin and fish skin as raw materials

[0132] Using animal skins such as pigskin and fish skin as raw materials, the preparation of collagen polypeptides is carried out according to the following steps. The process flow is as follows: image 3 shown.

[0133] 1) Pretreatment of raw materials Ⅰ: Wash the fish skin or dehaired pig skin with tap water, remove the fat attached to the skin, and then add 1%-3% of Sodium chloride (m / v), heated to 40°C-50°C, and soaked under this condition for 30-45 minutes, poured off the liquid, and cleaned it again with tap water;

[0134] 2) Raw material pretreatment II: Cut the fish skin or dehaired pig skin treated in step 1) into small pieces with a cutting machine, and then add 1% according to the solid-liquid ratio of 1:1-1:3 (m / v) Sodium hydroxide (m / v), heated to 70°C-80°C, and soaked under this condition for 20-30 minutes, poured off the liquid, and cleaned ...

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Abstract

The invention discloses a collagen polypeptide, a preparation method and application thereof. The collagen polypeptide comprises the following components in percentage by weight: 45-55 w / w% of components with molecular weight of 6500+ / -100 Da, 20-25 w / w% of components with molecular weight of 1300+ / -100 Da, and 25-35 w / w% of components with molecular weight of below 1000 Da. The nitrogen solubility index of the collagen polypeptide is 98.5-99.9%. The preparation method disclosed by the invention comprises the following steps: mixing the raw materials with complex enzyme A, and hydrolyzing.

Description

technical field [0001] The invention belongs to the technical field of enzyme catalysis, and in particular relates to a collagen polypeptide and its preparation method and application. Background technique [0002] Among the many animal proteins, collagen has high application value. According to someone's analysis, from lower invertebrates to higher mammals, there are more than 50 billion tons of collagen in nature, which is a major renewable resource. [0003] Collagen is mainly present in animals and is the main protein that constitutes the connective tissue of animals. Collagen is also the main component of extracellular matrix (ECM), accounting for about 85% of the solid matter of collagen fibers. In vertebrates, it accounts for about 25%-30% of the total protein in the body, equivalent to 6% of body weight. Mainly contained in bone, skin and tendons. Collagen contains a large amount of glycine, accounting for about 27% of the total amino acids, and the content of pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06A23L1/305A61K8/65A61K38/39A61P39/06A61P35/00A61P7/02A61P43/00A23L33/18
Inventor 叶秀云靳伟刚张洋罗鋆琳
Owner 香港百特有限公司
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