Method for separating clenobuterol hydrochloride through molecularly imprinted polymer
A clenbuterol hydrochloride and molecular imprinting technology, applied in the field of chemical engineering, can solve the problems of template residue and cross infection, and achieve the effects of high specificity and selectivity, improved sensitivity and high capacity
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Embodiment 1
[0034] 1. Preparation of Molecularly Imprinted Polymers
[0035]In step 1, 0.5 mol of the template molecule clenbuterol, the functional monomer methacrylic acid (MAA), and the crosslinking agent ethylene glycol dimethacrylate (EGDMA) were mixed according to a molar ratio of 1:2:15, Dissolve it in 6.5mL porogen (chloroform: acetonitrile = 3:10), put it into an ampoule, add 0.021g initiator azobisisobutyronitrile (AIBN), ultrasonicate for 10min, then fill with nitrogen for 5min, vacuum Sealed, 60 ° C water bath for 24 hours, obtained clenbuterol molecularly imprinted polymer;
[0036] Step 2: Take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 38 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) Soxhlet extraction were performed 3 times each to remove most of the template molecules, 6 hours each time, and finally Pure...
Embodiment 2
[0043] 1. Preparation of Molecularly Imprinted Polymers
[0044] In step 1, 0.5 mol of the template molecule clenbuterol, the functional monomer methacrylic acid (MAA), and the crosslinking agent ethylene glycol dimethacrylate (EGDMA) were mixed according to a molar ratio of 1:3:15, Dissolve in 5mL of porogen (acetonitrile: chloroform = 10:3), put it into an ampoule, add 0.021g of initiator azobisisobutyronitrile (AIBN), sonicate for 15min, then fill with nitrogen for 5min, and vacuum seal , in a 60°C water bath for 24 hours to obtain the clenbuterol molecularly imprinted polymer;
[0045] Step 2: Take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 45 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) were extracted by Soxhlet four times to remove most of the template molecules, each time for 5 h, and finally Pure me...
Embodiment 3
[0052] 1. Preparation of Molecularly Imprinted Polymers
[0053] Step 1, mix 0.5 mol of clenbuterol template, functional monomer methacrylic acid (MAA), and crosslinking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:6:15, and dissolve Put it into 8mL porogen (acetonitrile: chloroform = 10:3), put it into an ampoule, add 0.021g of initiator azobisisobutyronitrile (AIBN), ultrasonication for 10min, then fill with nitrogen gas for 5min, and vacuum seal. Water bath at 60°C for 24 hours to obtain clenbuterol molecularly imprinted polymer;
[0054] Step 2, take out the massive polymer, grind it with a mortar, sieve it with a sample sieve with a pore size of 54 μm, suspend it with acetone and discard the superfine particles. After drying the polymer, methanol: acetic acid (9:1, v / v) and methanol: formic acid (9:1, v / v) were extracted by Soxhlet 6 times each to remove most of the template molecules, 4 hours each time, and finally Pure methanol was ex...
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