Method for extracting and separating fusidic acid

A fusidic acid and fusidic acid separation technology, applied in the field of separation and extraction of fusidic acid, can solve the problems of inconvenient operation, environmental pollution, volatile cost, etc., and achieve the effects of simple operation, stable process and improved yield

Active Publication Date: 2010-08-04
JIANGSU JIUYANG BIOLOGICAL PHARMA
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although ion exchange technology has excellent separation selectivity, high concentration multiple, convenient operation, outstanding effect, and low extraction cost, the current yield and purity are not satisfactory, and it needs to be combined with other refining methods to obtain better results. result
The organic solvent extraction method is to use the difference in the distribution coefficient of fusidic acid and other substances in organic solvents. By adding a specific organic solvent to the fermentation broth for extraction, the separation of fusidic acid and other substances is achieved. This method uses The organic solvent is usually ether or various esters, and the process is relatively simple, but due to the large amount of organic solvent used, higher requirements are placed on the selectivity and regeneration ability of the solvent, and the use of this toxic, explosive and volatile solvent , causing serious pollution to the environment and inconvenience to the operation
At the same time, organic solvents are expensive, and their volatile characteristics also greatly increase the cost of industrial operation
[0004] Chinese patents 200680049547.4, 200810045003.1 and domestic patents RU1499922C and GB2224649A respectively disclose some production methods for the extraction and separation of fusidic acid. Therefore, there are many production methods for fusidic acid, but these methods are used in industrial production. there are many difficulties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Centrifugal filtration: Pump the fermented fusidic acid liquid that has been filtered normally into a centrifuge for separation, and transfer the centrifugate into a transfer storage tank as the raw material for the next step of ultrafiltration. (this step yield is greater than 98%)

[0026] 2. Ultrafiltration and impurity removal: pump 100L of centrifugate into the tubular ultrafiltration system for ultrafiltration and filtration, and continuously add pure water to dilute and rinse the concentrated solution to obtain ultrafiltration for removing residual mycelium, protein, sugar, pigment and other biological macromolecules. Filter the dialysate and 120L ultrafiltration concentrate containing residual mycelium, protein, sugar, pigment and other biological macromolecules. The membrane material used for ultrafiltration is a ceramic membrane with a molecular weight cut-off of 5000. The working conditions are: normal temperature, inlet pressure 3.5bar, outlet pressure 2....

Embodiment 2

[0033] 1, centrifugal filtration: same as embodiment one.

[0034] 2. Ultrafiltration and impurity removal: pump 100L of centrifugate into the tubular ultrafiltration system for ultrafiltration and filtration, and continuously add pure water to dilute and rinse the concentrated solution to obtain ultrafiltration for removing residual mycelium, protein, sugar, pigment and other biological macromolecules. Filter the dialysate and 120L ultrafiltration concentrate containing residual mycelium, protein, sugar, pigment and other biological macromolecules. The membrane material used for ultrafiltration is a ceramic membrane with a molecular weight cut-off of 10,000. The working conditions are: normal temperature, inlet pressure 4.5bar, outlet pressure 3.2bar.

[0035] 3. Resin adsorption and analysis: Adsorb 120L ultrafiltration concentrate through macroporous adsorption resin, wash the saturated resin with 15% ethanol aqueous solution, and then analyze with 35% ethanol solution to o...

Embodiment 3

[0041] 1, centrifugal filtration: same as embodiment one.

[0042] 2. Ultrafiltration and impurity removal: pump 100L of centrifugate into the tubular ultrafiltration system for ultrafiltration and filtration, and continuously add pure water to dilute and rinse the concentrated solution to obtain ultrafiltration for removing residual mycelium, protein, sugar, pigment and other biological macromolecules. Filter the dialysate and 120L ultrafiltration concentrate containing residual mycelium, protein, sugar, pigment and other biological macromolecules. The membrane material used for ultrafiltration is a ceramic membrane with a molecular weight cut-off of 20,000. The working conditions are: normal temperature, inlet pressure 5.5bar, and outlet pressure 3.6bar.

[0043] 3. Resin adsorption and analysis: Adsorb 120L ultrafiltration concentrate through macroporous adsorption resin, wash the saturated resin with 15% ethanol aqueous solution, and then analyze with 35% ethanol solution ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for extracting and separating fusidic acid. The method comprises the following steps: (1) extracting and separating fusidic acid concentrated solution by ultrafiltration; and (2) preparing fusidic acid crystal by the fusidic acid concentrated solution, wherein, a qualified fusidic acid product can be prepared by the fusidic acid concentrated solution through solvent extraction, decoloring, crystal separation, drying, crushing, screening, inspecting, finished product package and other production steps. The method has the advantages of short production process flow, high product purity, high yield, high automation degree, small environmental pollution, low production cost and the like, and is particularly suitable for industrial large-scale production of the fusidic acid.

Description

Technical field: [0001] The invention relates to a method for separating and extracting fusidic acid from fusidic acid fermentation. Background technique: [0002] Fusidic acid (Fusidic Acid, CAS6990-06-3) is a colorless crystal, containing half of crystal water, insoluble in water, soluble in organic solvents such as benzene, methanol, ethyl acetate, etc. It is extracted and separated from the fermentation broth of the fungus Ester, which is the compound with the most antibacterial activity among the fusidanes, and is widely used in the treatment of severe staphylococcal infections, such as septicemia, endocarditis, osteomyelitis, Traumatic infection and skin infection and other diseases. [0003] Fusidic acid is mainly prepared by fermentation. There are many methods for extracting and separating fusidic acid from fermentation broth, mainly including ion exchange method, organic solvent extraction method, etc. The ion exchange method uses fusidic acid and other substances...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07J13/00
Inventor 李军
Owner JIANGSU JIUYANG BIOLOGICAL PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap