Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system

Abstract

The invention discloses a screening and construction method for a composite microbial system for rapidly degrading rice straw and a composite microbial system, which belong to the technical field of microbial screening methods. The method comprises the following steps of: (1) constructing a strain pond; (2) preparing a solid culture medium; and (3) screening and constructing the composite microbial system. The composite microbial system screened by the method comprises bacteria and fungi. The method and the composite microbial system have the advantages that: the composite microbial system is obtained by screening under the condition of the solid culture medium, comprises the bacteria and the fungi and has better decomposition effect; the composite microbial system RSS-4 is screened under the condition of unsterilized solid culture medium; the screened composite microbial system has better decomposition effect at the intermediate temperature, namely the temperature of 22 DEG C; and the defect that the composite strain can only decompose at the temperature of 28 DEG C existing in the prior art is effectively overcome.

Description

Technical field [0001] The invention belongs to the technical field of microbial screening methods, and specifically relates to a method for screening and constructing a compound strain capable of rapidly degrading rice straw and the compound strain. Background technique [0002] As a large agricultural country, my country is rich in various crop straw resources. The annual crop straw output has reached more than 700 million tons, accounting for almost one-third of the world's total. In recent years, with the rapid development of the rural economy, the rural energy structure has also undergone fundamental changes. The vast majority of straw is no longer used as fuel. Burning or stacking or discarding crop straw has become a social problem. Crop straw is an important renewable biomass resource, containing elements such as potassium, silicon, nitrogen, calcium, magnesium, phosphorus, and organic components such as cellulose, hemicellulose, lignin, protein, and amino acids. Its nit...

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Problems solved by technology

[0010] However, the above-mentioned composite strains all have good straw degrading ability at room temperature and high temperature, and under liquid fermentation conditions, but there are few research reports on the composite strains decomposing crop straws under medium and low temperature conditions, especially under solid conditions.

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