Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system

A technology of rice straw and construction method, which is applied in the field of microbial screening, can solve the problems of few research reports and achieve good decomposition effect

Inactive Publication Date: 2010-12-01
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the above-mentioned composite strains all have good straw degrading ability at room temperature and high temperature, and under liquid fermentation condi

Method used

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  • Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system
  • Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system
  • Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system

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Experimental program
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Effect test

Embodiment 1

[0035] A method for screening and constructing a compound strain capable of rapidly degrading rice straw includes the following steps:

[0036] (1) Preparation of solid medium:

[0037] Rice straw powder (the rice straw is crushed and passed through a 60-mesh sieve) 75g, bran (after crushed and passed through a 60-mesh sieve) 30g, nutrient salt solution 100ml, stirred evenly, natural pH, and sterilized and non-sterilized ; Wherein the nutrient salt solution contains (NH4) 2SO4 2%, KH2PO4 0.1%, MgSO4.7H2O 0.5%, CaCl2 0.01%, NaCI 0.01%, FeSO4.7H2O 0.005%, MnS04.H2O 0.0016%, ZnSO4. 7H2O 0.0014% and CoCl 20.002%.

[0038] (2) Construction of strain pool:

[0039] Take twelve 5L plastic buckets, and fill them with 5kg paddy soil collected from Changping Farm of the Institute of Agricultural Sciences, Chinese Academy of Agricultural Sciences. Keep the moisture content at about 60%. Stir it evenly. Use rice straw as the only carbon source. Add urea and diphosphate. Potassium hydrogen and p...

Embodiment 2

[0044] This embodiment is exactly the same as embodiment 1, the only difference is that in step (3), the addition of inorganic nitrogen is used to adjust the C / N value in the solid medium to 28.

Embodiment 3

[0046] This embodiment is exactly the same as embodiment 1, except that in step (3), the addition of bran and inorganic nitrogen is used to adjust the C / N value of the solid medium to 28.

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Abstract

The invention discloses a screening and construction method for a composite microbial system for rapidly degrading rice straw and a composite microbial system, which belong to the technical field of microbial screening methods. The method comprises the following steps of: (1) constructing a strain pond; (2) preparing a solid culture medium; and (3) screening and constructing the composite microbial system. The composite microbial system screened by the method comprises bacteria and fungi. The method and the composite microbial system have the advantages that: the composite microbial system is obtained by screening under the condition of the solid culture medium, comprises the bacteria and the fungi and has better decomposition effect; the composite microbial system RSS-4 is screened under the condition of unsterilized solid culture medium; the screened composite microbial system has better decomposition effect at the intermediate temperature, namely the temperature of 22 DEG C; and the defect that the composite strain can only decompose at the temperature of 28 DEG C existing in the prior art is effectively overcome.

Description

Technical field [0001] The invention belongs to the technical field of microbial screening methods, and specifically relates to a method for screening and constructing a compound strain capable of rapidly degrading rice straw and the compound strain. Background technique [0002] As a large agricultural country, my country is rich in various crop straw resources. The annual crop straw output has reached more than 700 million tons, accounting for almost one-third of the world's total. In recent years, with the rapid development of the rural economy, the rural energy structure has also undergone fundamental changes. The vast majority of straw is no longer used as fuel. Burning or stacking or discarding crop straw has become a social problem. Crop straw is an important renewable biomass resource, containing elements such as potassium, silicon, nitrogen, calcium, magnesium, phosphorus, and organic components such as cellulose, hemicellulose, lignin, protein, and amino acids. Its nit...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/20C12R1/38C12R1/07C12R1/01C12R1/645
Inventor 沈德龙刘甲锋关大伟陈慧君李力姜昕李俊
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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