Compound mycelium capable of degrading sulpho-glucoside and applications thereof

Abstract

The invention discloses a compound mycelium capable of degrading sulpho-glucoside (glucosinolate for short). The compound mycelium is obtained by compounding two strains of mycete and a strain of candida spxopicalis, the two strains of mycete are screened out from rotten rapeseed dregs, and the sequence identification results are respectively as follows: Lichtheimia sp. JN3C and Aspergillus terreus, wherein the Lichtheimia sp. JN3C is collected by China Center for Type Culture Collection with the collection number of CCTCCM2010114; and the candida spxopicalis is obtained by courtesy of the College of Biological Engineering, Southern Yangtze University. The invention belongs to the technical field of microorganism application. The invention also discloses applications of the compound mycelium in degrading the glucosinolate and crude fibre in the rapeseed dregs and improving protein. The compounding proportion of the Aspergillus terreus to the Lichtheimia sp. JN3C to the candida spxopicalis is 0.6ml:0.8ml:1.0ml; after solid state fermentation, the degradation rate of the glucosinolate in the rapeseed dregs reaches above 90%, the protein content is increased to 48.47% (dry basis), and the crude fibre content is reduced to 2.78% from 14.5% (dry basis); and the compound mycelium has favorable application value in biological detoxification and quality improvement of the rapeseed dregs.

Description

technical field [0001] The invention relates to a compound bacterial strain for degrading glucosinolate and its application, and belongs to the technical field of applied microorganisms. technical background [0002] Rapeseed meal contains high protein content. However, because rapeseed meal contains anti-nutritional factors such as phytic acid, tannin, and crude fiber, especially glucosinolates (glucosinolates for short). More than 120 kinds of glucosinolates have been found in hundreds of plants. Glucosinolates can cause goiter in feeding animals, resulting in growth retardation, which makes it difficult for rapeseed meal to be fully used as a high-quality feed protein resource. Therefore, it is particularly important to remove glucosinolates from rapeseed meal. At present, methods for removing glucosinolates include physical methods, chemical methods, biological methods, and genetic methods. Physical and chemical methods often have better glucosinolate removal effects, ...

AI Technical Summary

Problems solved by technology

[0003] However, glucosinolates are a class of substances containing different hydrocarbon groups. The hydrocarbon groups can be alkane, olefin, and aromatic hydrocarbons, etc., and enzymes are highly specific. Often, an enzyme can only degrade one of the substances. The enzyme system produced by the solid-state fermentation of single bacteria is relatively simple, which often results in low glucosinolate degradation efficiency. At the same time, the current research on the degradation of glucosinolates in rapeseed meal does not consider the impact on the content of tannin, phytic acid, crude fiber and protein.

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