Microorganism-embedded carrier with composite nanostructure and preparation method thereof

A microbial carrier and nanocomposite technology, which is applied in the direction of being fixed on/in an organic carrier, can solve the problems of inability to use microbial carriers and poor mechanical properties of polymer gels, and achieves reduced direct contact, excellent mechanical properties, Not easy to break effect

Inactive Publication Date: 2011-01-19
BEIJING SOUND ENVIRONMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a nano-composite structure-embedded microbial carrier and its preparation method, which can solve the problem that the existing common polymer gel has poor mechanical properties, and the prepared microbial carrier cannot withstand strong impact for a long time. Application in the environment of external force

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a microbial carrier, which is a nano-composite structure embedded microbial carrier. The microbial carrier takes various raw materials according to the following proportions, including:

[0029] Raw material dosage

[0030] Acrylamide (water-soluble monomer capable of radical polymerization) 8wt%

[0031] Clay 0.8wt%

[0032] Ammonium persulfate and sodium bisulfite (initiator) 0.05wt%

[0033] N, N-methylenebisacrylamide (crosslinking agent) 0.10wt%

[0034] Bacterial powder (microorganism and / or enzyme) 0.25wt%

[0035] Sterile water 90.8wt%;

[0036] The preparation method of the microbial carrier is as follows: first fully disperse the clay particles in water, then add acrylamide and N,N-methylenebisacrylamide and stir thoroughly, then add bacterial powder, ammonium persulfate and sodium bisulfite to mix Obtain the gel solution evenly, put the gel solution in a sealed container under normal pressure, and place it at 30°C for 1 hour to o...

Embodiment 2

[0038] This embodiment provides a microbial carrier, which is a nano-composite structure embedded microbial carrier. The microbial carrier takes various raw materials according to the following proportions, including:

[0039] Raw material dosage

[0040] Acrylamide (water-soluble monomer capable of radical polymerization) 10wt%

[0041] Clay 1wt%

[0042] Potassium persulfate and sodium bisulfite (initiator) 0.3wt%

[0043] N, N-methylenebisacrylamide (crosslinking agent 1) 0.16wt%

[0044] Polyethylene glycol 400 diacrylate (crosslinking agent 2) 0.02wt%

[0045] Bacterial powder (microorganism and / or enzyme) 2.5wt%

[0046] Sterile water 86.02wt%;

[0047] The preparation method of the microbial carrier is as follows: first fully disperse the clay particles in water, then add acrylamide and N, N-methylenebisacrylamide and stir thoroughly, then add microbial preparations, potassium persulfate and sodium bisulfite and mix evenly To obtain the gel solution, put the gel sol...

Embodiment 3

[0049] This embodiment provides a microbial carrier, which is a nano-composite structure embedded microbial carrier. The microbial carrier takes various raw materials according to the following proportions, including:

[0050] Raw material dosage

[0051] Acrylamide (free radical polymerizable water-soluble monomer 1) 6wt%

[0052] Hydroxyethyl methacrylate (free radical polymerizable water-soluble monomer 2) 8wt%

[0053] Clay 8wt%

[0054] Ammonium persulfate and sodium bisulfite (initiator) 0.6wt%

[0055] N, N-methylenebisacrylamide (crosslinking agent 1) 0.1wt%

[0056] Polyethylene glycol (400) methacrylate (crosslinking agent 2) 0.15wt%

[0057] Aqueous dispersion of nitrifying bacteria (water dispersion of microorganisms and / or enzymes, wherein the content of microorganisms and / or enzymes is 1wt% by solid, and the remaining 29wt% is water) 30wt%

[0058] Water 47.15wt%;

[0059] The preparation method of the microbial carrier is as follows: first fully disperse t...

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Abstract

The invention discloses a microorganism-embedded carrier with composite nanostructure and a preparation method thereof. The microorganism carrier is hydrogel in which active microorganisms are embedded and which is prepared by polymerization reaction and has a composite nanostructure. The preparation method mainly comprises the following steps of: 1, fully dispersing clay particles in water; 2, adding amide monomer into the mixture and fully stirring the mixture; 3, adding a microorganism preparation and a free radical polymerization initiator into the mixture and uniformly stirring the mixture; and 4, performing the free radical polymerization in a sealed container at the normal temperature and the normal pressure to obtain the hydrogel, namely the nano composite microorganism-embedded carrier. The compression ratio of the nano composite microorganism carrier of the embodiment of the invention can reach 97 percent. The method preparing the microorganism-embedded carrier has the advantages of simple, convenient and practical process, and stable quality and superior mechanical property of the prepared carrier.

Description

technical field [0001] The invention relates to the field of immobilized organisms, in particular to an embedded microorganism carrier with a nanocomposite structure and a preparation method thereof. Background technique [0002] Immobilized Biotechnology (Immobilized Biotechnology) is a new technology that has developed rapidly since the 1960s. It uses chemical or physical means to locate free cells or enzymes in a limited space area, so that free cells or enzymes Remain active and can be used repeatedly. In immobilized biotechnology, each gel bead can be regarded as a small bioreactor except for the microorganisms used. Due to the shortened diffusion distance, the conversion rate is significantly improved, and the cells can be recovered and reused after the reaction. [0003] In the field of environmental protection, immobilized biotechnology has the advantages of high microbial density, rapid response, less microbial loss, easy separation of products, and easy control of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/04
Inventor 李宗慧屈佳玉王凯刘金泉佟娟
Owner BEIJING SOUND ENVIRONMENTAL ENG
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