Lactobacillus plant arum ZY-40 and application thereof to preparing fish sausage through fermentation
A technology of Lactobacillus plantarum and ZY-40, which is applied in the fields of application, food preparation, and microbial-based methods to achieve good fermentation flavor and gel structure, good sliceability and chewiness
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Example 1: Screening, Identification and Preservation of Bacterial Strains
[0026] (1) MRS liquid medium: peptone 10g, beef extract 10g, yeast extract 5g, triamine citrate 2g, glucose 20g, Tween-80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfuric acid Manganese 0.25g, distilled water 1000ml, pH value 6.2~6.4.
[0027] (2) Isolation of strains: under aseptic conditions, distiller's lees fish meat was added to sterilized saline, homogenized under aseptic conditions, after gradient dilution, poured onto plates by pouring method, and incubated at 30°C for 48 hours. Pick a single colony and continue plating until purified. Gram-positive and catalase-negative strains were suspected lactic acid bacteria.
[0028] (3) Re-screening of strains: Carry out glucose fermentation gas production, viscous substance formation, hydrogen peroxide production, hydrogen sulfide production, amino acid decarboxylase, arginine ammonia production, sal...
Embodiment 2
[0034] Embodiment 2: Application of Lactobacillus plantarum ZY-40 in the preparation of fermented fish sausage
[0035] The processing technology mainly includes: surimi preparation → adding auxiliary materials, chopping → inoculation → filling → fermentation and maturation → packaging
[0036] The specific operation is as follows:
[0037] (1) Preparation of surimi: thaw the frozen hairtail, dissect it, remove the scales, skin, head, tail and viscera, wash and mince the meat.
[0038] (2) Chop and mix: Add quality 3% starch, 1.5% sucrose, 2% glucose and 2% salt at low temperature (5-10° C.), chop and mix for 20 minutes.
[0039](3) Liquid culture of Lactobacillus plantarum ZY-40: after activation, Lactobacillus plantarum ZY-40 was inoculated in MRS liquid medium, cultivated to late logarithmic growth at a temperature of 20-40°C and a rotating speed of 150rpm, and measured and Adjust its cell concentration.
[0040] (4) Inoculation: Add Lactobacillus plantarum ZY-40 to the ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com