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Human placental anti-aging active protein as well as separation method and application thereof
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An active protein, anti-aging technology, applied in peptide/protein components, applications, botanical equipment and methods, etc., can solve problems such as difficult to achieve protein concentration
Inactive Publication Date: 2011-04-27
葛龙海
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The results of protein folding studies in test tubes may not truly reflect the folding process of proteins in vivo. For example, the protein concentration in mitochondria is very high, and it is difficult to achieve such a high protein concentration in test tubes.
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Embodiment 1
[0026] Example 1 Cloning of human Oct4, Sox2, Klf4, NANOG, c-Myc, LIN28, TOR gene and (or) TOR domain gene
[0027]These genes were cloned from human placental tissue. Their Genebank numbers are: S81255.1; NM_003106.2; NM_004235.4; NM_024865.2; CAA25288.1; NP_078950.1; P42345.1; P42345.1. Taking Oct4 as an example for human cloning, the specific steps are as follows:
[0059] Example 2 In bacteria, yeast and (or) eukaryotic cells, a high-throughput screening platform is established for screening proteins that can interact with the above-mentioned target genes in vivo
[0060] Construct fusion protein expression plasmids of human Oct4, Sox2, Klf4, NANOG, c-Myc, LIN28, TOR and GFP. GFP is at the C-terminus of Oct4, Sox2, Klf4, NANOG, c-Myc, LIN28, TOR. By observing the changes in the intensity of GFP green fluorescence in bacteria, yeast and (or) eukaryotic cells, the expression level of upstream proteins and the changes in protein folding are monitored. ( figure 1 , Promoter represents the promoter, Escherichia coli uses the T7 promoter, yeast Pichia uses the AOX1 promoter, and CHO cells use the CMV promoter).
Embodiment 3
[0061] Example 3 Screening of human placental active proteins that specifically interact with Oct4, Sox2, Klf4, NANOG, c-Myc, LIN28, and TOR proteins
[0062] Extraction of total RNA from human placenta: Total RNA was extracted according to the instructions of the Boehringer Mannheim kit. Take out the human placenta from the liquid nitrogen, weigh 1g and crush it, put it in a mortar quickly, add liquid nitrogen to grind it into powder, transfer it to a homogenizer, wait until the liquid nitrogen is completely evaporated, add 10ml TripureTM Isolation Reagent to fully homogenate, add 2ml Mix well with chloroform, let it react for 5-10 minutes, centrifuge to take the supernatant, add 5ml of hexylpropanol to mix and let stand for 10min, centrifuge to collect the precipitate, wash with 75% ethanol to collect the precipitate and dry it, dissolve RNA with 515μl DEPC water, take 10μ1 for UV For quantitative analysis, determine the RNA concentration, take 5 μl for agarose gel electroph...
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Abstract
The invention discloses a human placental anti-aging active protein as well as a separation method and an application thereof. The amino acid sequence of the human placental anti-aging active protein is disclosed in SEQ ID NO: 1. The separation or cloning method comprises the following steps: (1) cloning a human target gene or target gene structure domain; (2) in bacteria, yeast or eukaryotic cells, building a high-flux screening platform; (3) building a human placental cDNA library; and (4) inducing the built human placental cDNA library into the high-flux screening platform built in step (2), adopting a visualized method characterized by protein folding and mutual action, and screening the human placental protein causing the protein folding variation of the target gene or target gene structure domain; and obtaining the human placental anti-aging active protein. The human placental anti-aging active protein provided by the invention has the characteristic that in-vitro amplification of marrow hemopoietic stem cells / hemopoietic progenitor cells are effective and cheap. The human placental anti-aging active protein in the invention can prolong the service life of mice by about 15%, and can be used for preparing anti-aging or antioxidant drugs or health care products.
Description
technical field [0001] The present invention relates to an anti-aging active protein, in particular to an active protein that is isolated and cloned from human placenta and has the function of regulating cell self-renewal. The present invention also relates to a method for separating and cloning the active protein. The present invention further relates to The application of the active protein in anti-aging belongs to the field of anti-aging active protein. Background technique [0002] Human placenta contains a variety of original life active substances and nutritional components, and more importantly, it contains all the active substances and all the nutritional components required for the occurrence and development of human stem cells - the origin cells of the human body. Stem cells have the ability of self-replication and multi-differentiation potential, can form cells of various tissues and organs of the human body, and can directional restore damaged, aging, degenerated...
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