Bacillus amyloliquefacien and preparation method of polluted water reparation agent by using same

A technology for the preparation of amylolytic spores and strains, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., and can solve problems such as complex production processes

Active Publication Date: 2011-04-27
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation uses complex bacteria to complete the digestion or degradation of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and COD in aquaculture water, and cannot effectively treat the organic matter, ammonia nitrogen and nitrate in the sediment. Pollutants have the function of degradation and digestion. In addition, the use of complex flora as a preparation will inevitably cause more complicated problems in the production process.

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  • Bacillus amyloliquefacien and preparation method of polluted water reparation agent by using same
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Examples

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Embodiment 1

[0105] A method for preparing a polluted water restoration agent by using Bacillus amyloliquefaciens as a strain, the method comprising the following process steps:

[0106] A. Inoculate the activated Bacillus amyloliquefaciens CGMCCNo.3992 (Bacillus amyloliquefaciens) into a medium containing a carbon source after expanded culture, and add necessary nitrogen sources and nutrients;

[0107] B. Fermentation is carried out by the method of liquid deep aeration stirring fermentation + solid carrier adsorption;

[0108] C. Drying the fermented material after the fermentation is completed to prepare a polluted water restoration agent.

[0109] The activation process steps of the bacterial classification in the step A are as follows:

[0110] Inoculate Bacillus amyloliquefaciens CGMCC No.3992 (Bacillus amyloliquefaciens) on the slant of beef extract peptone medium test tube, culture at a temperature of 28°C-30°C for 48 hours, and transfer repeatedly 2-3 times to activate the strain...

Embodiment 2

[0123] Step A of the present embodiment is the same as step A of embodiment 1;

[0124] The medium in steps A and B is composed of 0.2% tryptone, 0.2% glucose, 0.2% yeast extract, 0.4% dipotassium hydrogen phosphate, 0.03% manganese sulfate and the rest of the water by mass percentage, and the conditions for fermentation and culture are the same as Example 1 is the same. After the fermentation is terminated, 1.0 times the volume of powdered zeolite and 1.0 times the volume of peat powder are added to the fermentation broth for adsorption, and air-dried at a temperature lower than 100°C until the water content of the material is below 12% by mass to obtain a polluted water restoration agent.

Embodiment 3

[0126] The strain activation step of step A is the same as that in Example 1. In the expanded culture, the expanded culture medium is composed of 0.3% tryptone, 0.3% glucose, 0.3% yeast extract, 0.4% dipotassium hydrogen phosphate, 0.03% manganese sulfate and excess Amount of water composed of natural pH. Dispense into Erlenmeyer flasks, and the sterilization condition of the expansion medium is 121°C autoclaving for 30 minutes, cooling to 25°C-35°C, inoculating Bacillus amyloliquefaciens strain CGMCCNo.3992 (Bacillus amyloliquefaciens), at 25°C- Shake flask culture at 30°C for 48 hours;

[0127] The solid fermentation medium in step B is respectively 10% crushed bran by mass percent, 88% powdery medical stone (0.1-2mm in particle diameter), 1.0% corn flour, 0.03% dipotassium hydrogen phosphate, 0.25 % ammonium sulfate, 1.0% humic acid and the balance of water. The sterilization condition of the solid fermentation medium is: autoclave at 121° C. for 60 minutes. After the ma...

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Abstract

The invention belongs to microbial strains and microbial agent, in particular to a bacillus amyloliquefacien and a preparation method of a polluted water reparation agent by using the same. The method comprises the following steps: carrying out the amplification cultivation on the bacillus amyloliquefacien CGMCC No.3992 after activation, then inoculating the bacillus amyloliquefacien to a medium containing a carbon source, and adding a necessary nitrogen source and necessary nutritive materials; fermenting by using the method of combining the liquid deep aeration and agitation with solid carrier adsorption, or the method of solid fermentation; and drying the material after the fermentation to obtain the polluted water reparation agent. According to the invention, the purification problem of polluted water and substrate sludge in the prior art is solved; the invention has following the advantages: the effective viable count and sporulation rate in the polluted water reparation agent are high, the crossbred pollution is less, the process is simple, the cost is low, the effect is stable and durable and the like. The bacillus amyloliquefacien provided by the invention has significant digestion effect on the organics, ammonia nitrogen and nitrate nitrogen in the substrate sludge and the water.

Description

technical field [0001] The invention belongs to microbial strains and microbial preparations, in particular to a bacillus amyloliquefaciens and a method for preparing a polluted water restoration agent by using the strain. Background technique [0002] The shortage of water resources is one of the survival problems that all mankind has to face. my country's per capita fresh water resources are only 2,300 cubic meters, which is only 1 / 4 of the world's average level. It is one of the 13 countries with the poorest per capita water resources in the world. Even so, the degree of pollution of water resources is very serious. According to monitoring, 39% of the country's lakes and southeast coastal water bodies are eutrophic, and more than 70% of the rivers are polluted to varying degrees. The groundwater of most cities in the country is subject to a certain degree of point source and non-point source pollution, and there is a trend of increasing year by year. In addition, my coun...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 王占武张翠绵李洪波贾楠胡栋朱桂艳李洪涛杨晶
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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