Method for producing hydrogen by fermenting special anaerobic clostridium butyricum

A technology of anaerobic Clostridium butyricum and anaerobic bacteria, which is applied in the field of hydrogen production by fermentation of obligate anaerobic Clostridium butyricum, can solve the problems of complex operation, anaerobic fermentation conditions, and difficult promotion, and achieve broad application prospects , Low requirements for fermentation environment conditions, high hydrogen production efficiency

Inactive Publication Date: 2011-05-04
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through patent search, the existing patents are mainly research on fermentation substrates, fermentation equipment and improving hydrogen production efficiency, such as a kind of sludge and organic waste mixed biological production Hydrogen method (application number: 200710032658.0 Zhou Shaoqi, etc.), photosynthetic microbial hydrogen production reactor (application number: 200620032282.4), biological fermentation hydrogen production device (application number: 00231651.X Shen Jianquan), a CO2 sub>The method of rapidly multiplying algae and directly using it for biological hydrogen production (200710010804.X) and the expanded bed equipment for high-efficiency fermentation biological hydrogen production (application number: 03260012.7, Ren Nanqi, etc.), etc., and for fermentation strains, especially high-efficiency hydrogen-producing bacteria The screening research on species is relatively weak, and there is only one biohydrogen production method and its special bacteria (application number: 200910088408.8 Xing Xinhui, etc.), but the bacteria used in this method are recombinant bacteria after gene introduction, the operation is more complicated, and the fermentation conditions require Strictly anaerobic, not easy to promote

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  • Method for producing hydrogen by fermenting special anaerobic clostridium butyricum
  • Method for producing hydrogen by fermenting special anaerobic clostridium butyricum
  • Method for producing hydrogen by fermenting special anaerobic clostridium butyricum

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Selection and Determination of Strain Anaerobic Operation Technology

[0029] Prepare basal medium:

[0030] (1) Scheme 1: The components of the culture medium are: peptone 10g / L, beef extract 2.4 g / L, yeast extract 5.0g / L, Na 2 HPO 4 4g / L, L-cysteine ​​0.2 g / L, L-cysteine ​​hydrochloride monohydrate 0.5 g / L, distilled water 1000mL. Adjust the pH to 7.0, put each 100mL of culture solution into a 250mL Erlenmeyer flask, wrap the inner layer with gauze, and wrap the outer layer with newspaper to seal. Sterilize at 120°C for 25 minutes, dry in an oven at 60°C, inoculate the strain Clostridium butyricum into the bottle containing the culture medium (sterile anaerobic operation), and culture at 37°C for 24-48 hours.

[0031](2) Scheme 2: The components of the above-mentioned culture medium are basically the same, in addition, 1 mL / L of resazurin indicator (0.2%) is added. Adjust the pH to 7.0, put each 100mL of culture solution into a 250mL saline bottle (...

Embodiment 2

[0035] Example 2: Determination of selection of strain fermentation environment

[0036] The components of the fermentation medium are: peptone 10g / L, beef extract 2.4 g / L, yeast extract 5.0g / L, Na 2 HPO 4 4g / L, L-cysteine ​​0.2g / L, L-cysteine ​​hydrochloride monohydrate 0.5g / L, resazurin (0.2%) 1mL / L, glucose 10g / L; Initial pH=7.0; During the preparation of the culture medium, use conventional heating and boiling to expel dissolved oxygen in the liquid phase and use nitrogen to blow off the gas phase air in the bottle to ensure strict anaerobic sterilization at 115°C for 25 minutes; sterile anaerobic operation , Inoculate Clostridium butyricum high-concentration bacterial solution (dry cell weight up to 0.658g / L) in 200ml medium at a volume ratio of 1:10 to obtain a fermentation broth, and culture it statically in an anaerobic box at 37°C:

[0037] (1) Option 1: Take a bottle of inoculated fermentation broth, the total amount is 220mL, take out the anaerobic box after s...

Embodiment 3

[0042] Embodiment 3: Novel Clostridium butyricum fermentation hydrogen production

[0043] The components of the fermentation medium are: peptone 10g / L, beef extract 2.4 g / L, yeast extract 5.0g / L, Na 2 HPO 4 4g / L, L-cysteine ​​0.2g / L, L-cysteine ​​hydrochloride monohydrate 0.5g / L, resazurin (0.2%) 1mL / L, glucose 10g / L; Initial pH=7.0; during the preparation of the culture medium, use heating and boiling to expel dissolved oxygen in the liquid phase and use nitrogen to blow off the gas phase air in the bottle to ensure strict anaerobic sterilization at 115°C for 25 minutes; high-concentration Clostridium butyricum bacteria liquid Inoculate in 200ml fermentation medium at a ratio of 1:10, perform sterile anaerobic operation, and culture statically in an anaerobic box at 37°C:

[0044] (1) Scheme 1: Inoculate Clostridium butyricum JCM1391 into a bottle of fermentation broth, the total amount is 220mL, take out the anaerobic box after sealing, shake the culture on a shaking ...

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Abstract

The invention provides a method for producing hydrogen by fermenting special anaerobic clostridium butyricum. The screened special anaerobic bacterium is clostridium butyricum JCM1391. Proven by tests, the hydrogen is produced by fermenting by batch by using anaerobic bacteria under the sterile anaerobic condition, wherein the mixture of 10.0-15.0g of peptone, 2.0-3.0g of beef extract, 5.0-10.0g of yeast extract, 3.0-4.0g of Na2HPO4, 0.2-0.5g of L-cysteine, 0.2-0.5g of L-cysteine hydrochloride monohydrate, 1 ml of resazurin indicator which accounts for 0.2 percent by weight and 1 ml of distilled water is used as culture media, and glucose is used as fermentation matrix. The method has the advantage of high hydrogen production efficiency and low requirement on environmental condition for fermentation.

Description

technical field [0001] The invention relates to a method for producing hydrogen by utilizing microbial anaerobic fermentation, in particular a method for producing hydrogen by utilizing highly efficient anaerobic bacteria. Background technique [0002] The combustion calorific value of hydrogen is three times that of gasoline, and the only combustion product is water, which has no pollution to the environment. Therefore, hydrogen energy can be described as a clean, efficient and renewable green energy. [0003] Hydrogen energy is not a primary energy source and needs to be produced from hydrogen-containing compounds. At present, more than 90% of the hydrogen in the world comes from fossil combustion, and the rest is hydrogen production from water electrolysis. No matter what kind of hydrogen production method will directly or indirectly consume a large amount of fossil energy. The reserves of fossil fuels are limited and tend to be exhausted. At the same time, greenhouse g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/145
Inventor 钱春香陈璐圆王瑞兴
Owner SOUTHEAST UNIV
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