Trichoderma spore powder as well as preparation method and application thereof

A technology of spore powder and spore powder, which is applied in the field of Trichoderma spore powder and its preparation, can solve the problems of unreasonable application and maintenance of microbial activity, and achieve the effects of easy promotion, high density of Trichoderma spores, and reduced preparation costs

Inactive Publication Date: 2013-02-20
惠州市南天生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because people have not yet found a solution to how to maintain microbial activity under the high temperature conditions of dry spray technology, so far spray drying technology has not been rationally applied in the field of microbial active pesticide preparation

Method used

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  • Trichoderma spore powder as well as preparation method and application thereof
  • Trichoderma spore powder as well as preparation method and application thereof
  • Trichoderma spore powder as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Recovery and expansion of strains

[0030] (1) The refrigerated Trichoderma asperellum T1 (Trichoderma asperellum T 1 ) is planted on the PDA medium to make it recover;

[0031] (2) Adopting yeast extract sucrose medium, and setting the culture temperature as 30°C and humidity as 70-85%, the bacterial strains revived in step (1) are subcultured for 48-72 hours on a plate until they grow long A dark green colony with a diameter of about 5-7mm was produced;

[0032] (3) Wash the bacterium colonies formed by the plate subculture in step (2) with sterile water, and inoculate them in an eggplant-shaped bottle filled with high-temperature sterilized compost consisting of bran, sawdust, sucrose, vitamin B and water Carry out the spore expansion culture in the medium, set the culture temperature at 30°C, the humidity at 70-85%, and the culture time for 5-7 days. Turn and beat the eggplant-shaped bottle every day until white hyphae grow on the culture and grow on it. Green...

Embodiment 2

[0039] The following further describes the influence of changes in parameters such as inlet air temperature, feed amount, type of drying aid, and content of drying aid on the preparation of Trichoderma spore powder.

[0040] Taking the germination rate as an indicator, the single factor analysis was carried out on the inlet air temperature and feed amount of spray drying. The influence of different inlet air temperature and feed amount on the prepared spore powder was measured by changing the inlet air temperature and feeding amount during the preparation process. Therefore, the comparison and selection of the inlet air temperature and the range of feed amount are beneficial to ensure high germination rate of spores and good drying effect. When the feed amount is 5wt%, set five inlet air temperatures of 90°C, 100°C, 110°C, 120°C and 130°C, measure the germination rate of the spore powder prepared at each temperature and record the drying effect; When the wind temperature is 12...

Embodiment 3

[0049] The L9 (3 4 ) Orthogonal test, the germination rate, collection rate, powder collection rate and live spore content of the product are used as evaluation indicators to select an appropriate preparation process. In this experiment, L9 (3 4 ) orthogonal test, as shown in Tables 3 and 4:

[0050] Table 3 Orthogonal test factor level table of spore powder formulation optimization

[0051]

[0052] Table 4 Orthogonal test results of spore powder formulation process

[0053]

[0054]

[0055] Note:

[0056]

[0057] It can be seen from Table 4 that in A 1 B 1 C 1 D 2 Under the formulation process, that is, 5wt% of β-cyclodextrin, inlet air temperature of 110°C, and feed amount of 10wt%, the content of live spores in the product is the highest. By comparing the range (R) of the index, the primary and secondary order of the factors affecting the content of live spores is A>C>D>B, that is, the type of drying aid is the main influencing factor, followed by ...

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Abstract

The invention discloses a trichoderma spore powder as well as a preparation method and application thereof. The trichoderma spore powder contains trichoderma spore powder and a drying aid, wherein the trichoderma spore powder is the spore powder of Trichoderma sperellum T1 which is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO: 3531 and a rDNA ITS region segment sequence shown in SEQ ID NO:1; the drying aid is one or more of beta-cyclodextrin, potato starch and soluble starch. The trichoderma spore powder has an effective prevention and control effect on banana vasicular wilt, and has a remarkable antagonism effect on various pathogenic funguses, especially on soil-borne funguses. The preparation time is saved with the preparation method of the trichoderma spore powder, which can be used for preparing the trichoderma spore powder which has high density, high germination rate and high drying efficiency and is easy to store, transport and use.

Description

technical field [0001] The invention relates to a microbial active pesticide powder, in particular to a trichoderma spore powder and its preparation method and application. Background technique [0002] Trichoderma belongs to the subphylum Deuteromycetes, the order Conglomerate, and belongs to the family Stemaceae. It is a class of biocontrol and beneficial bacteria with important economic significance. Trichoderma has strong adaptability to the environment, grows much faster than pathogenic fungi, and is a strong competitor for the living space and nutrient source of plant pathogenic fungi in soil. Trichoderma can effectively use low-concentration nutrients on the plant surface or near the invasion point of plant pathogenic fungi to quickly occupy the space and absorb nutrients so that plant pathogenic fungi cannot invade. In recent years, the research on artificially introducing Trichoderma into the soil to control plant diseases has been active, such as using Trichoderma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/12A01P3/00
Inventor 方羽生钟平生张颂声赵瑾詹玉海周俐蒋刚彪李小妮高永峰
Owner 惠州市南天生物科技有限公司
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