Tabanusyao Macquart antithrombotic enzyme tablysin and gene and application thereof

A gadfly protease and gene technology is applied in the field of biomedicine to achieve the effects of inhibiting the formation of thrombus in the tail vein of rats and inhibiting the hydrolysis of fibrinogen
CN102071184AActive Publication Date: 2011-05-25KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Publication Date
2011-05-25

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Abstract

The invention relates to Tabanusyao Macquart antithrombotic enzyme tablysin and a gene and application thereof, and belongs to the field of biomedicine. In the invention, a natural antithrombotic enzyme is separated by a biomechanical method and a coded gene of the enzyme is amplified; and a gene engineering product which has equivalent activity with natural protein is obtained by using an escherichia coli expression system. The Tabanusyao Macquart protease tablysin is active protein which is separated from a Tabanusyao Macquart salivary gland, wherein the molecular weight is 27KDa; and an amino acid sequence of the enzyme is shown as SEQ ID NO. 1. The gene of the Tabanusyao Macquart protease tablysin consists of a nucleotide sequence which is shown as SEQ ID NO. 2. Through the TabanusyaoMacquart protease tablysin, fibrinogen can be hydrolyzed, platelet aggregation can be suppressed, and carrageenin-induced rat caudal vein thrombosis can be suppressed; and the Tabanusyao Macquart protease tablysin does not have bleeding activity and can be applied to preparation of medicaments for treating thrombotic diseases.
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Description

Technical field:

[0001] The invention provides gadfly antithrombotic enzyme tablysin and its gene and application, belonging to the technical field of biomedicine. Background technique:

[0002] Thromboembolic disease seriously affects human health and is one of the causes of the highest mortality. In the past few decades, the antithrombotic drugs that have been developed mainly include thrombolytic drugs, anticoagulant drugs and antiplatelet drugs. However, many antithrombotic drugs are often limited by systemic side effects including hypotension and bleeding. Therefore, it is necessary to develop new antithrombotic drugs with more potential and specificity.

[0003] Research in traditional Chinese medicine has shown that many natural components derived from animals and plants have definite antithrombotic effects. Ancient medical books such as "Compendium of Materia Medica" and "Synopsis of the Golden Chamber" have recorded many prescriptions for promoting blood circulatio...

Claims

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