Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paenibacillus elgii strain and application thereof

A technology of bacillus and spores, applied in the field of agricultural biology

Active Publication Date: 2011-06-08
常熟紫金知识产权服务有限公司
View PDF3 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And organic fertilizers with added chemicals are not real bio-organic fertilizers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paenibacillus elgii strain and application thereof
  • Paenibacillus elgii strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the acquisition of functional strain

[0023] Collect the rhizosphere soil of eggplant plants, and use the dilution plate method to spread the soil dilution on the potato dextrose-peptone medium with eggplant Verticillium dahliae spores, and obtain 24 bacterial strains that produce inhibition zones, among which 8 strains were examined by microscope Spore-forming bacterial strains. Purified strains were further screened from 8 strains to screen out the strain FQ35 which had a large inhibition zone against Verticillium dahliae and could produce a large amount of exopolysaccharide. The strain was identified as a species of Paenibacillus by morphological, physiological and biochemical identification ( paenibacillus sp.); after 16S rDNA sequence analysis, under the premise of extracting colony DNA, a clear band of 1442bp was amplified with bacterial universal primers, sequenced and compared with Genbank Paenibacillus elgii The similarity is more than 98%. ...

Embodiment 2

[0025] Embodiment 2: FQ35 bacterial strain is to the mensuration of pathogenic microorganism such as plant soil-borne pathogenic bacteria antibacterial spectrum

[0026] After being activated by beef extract peptone medium, the strains preserved on the slant of FQ35 strain were inoculated on the potato glucose plate, and plant pathogenic bacteria were inoculated at the same time. The antibacterial effect of FQ35 strain was observed. Bacteria (pathogenic bacteria) were cultured with mixed bacteria, and then planted and inoculated with FQ35 strain to observe the antibacterial effect.

[0027] It can be seen from Table 1 that the FQ35 strain has inhibitory effects on the 10 plant pathogenic bacteria detected (tomato R. solanacearum is a bacterial pathogen, and the rest are fungal pathogens) and three strains of environmental pollution indicator microorganisms.

[0028] Table 1. Determination results of antibacterial spectrum of FQ35 strain

[0029] Detected strains

[...

Embodiment 3

[0031] Embodiment 3: the optimization of FQ35 bacterial strain liquid fermentation condition:

[0032] (1) Connect one loop of the FQ35 strain preserved on the slant to the seed culture solution, fill a 250mL triangular bottle with a volume of 50mL, and culture at 31°C and 180r / min for 36h with shaking (the amount of bacteria is 3.5×10 8 cfu / mL).

[0033] (2) Use fermentation medium, 250mL Erlenmeyer flask with 50mL volume, shaking flask speed 180r / min, inoculum size 2%, temperature 31°C, culture time 48h, respectively detect different carbon sources, nitrogen sources and inorganic salts on the FQ35 strain The influence of the antibacterial effect of the fermentation broth was repeated 3 times.

[0034] (3) For 9 common carbon sources: corn flour, corn starch, maltose, glucose, molasses, lactose, sucrose, xylose, soluble starch (concentration is 3g / L), compared with CK (without carbon source) . The results showed that when sucrose or corn starch was used as carbon source, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
water absorptionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a paenibacillus elgii strain CGMCC No.4231. The microbial inoculant or moisture-retention organic fertilizer prepared from the strain has the functions of preventing the plant diseases, reducing the water loss of plant rhizosphere, strengthening the soil aggregate, improving the soil and promoting the plant growth. The bacteriostatic drought-resisting organic fertilizer is suitable for various crops and cash crops cultivated in arid areas and in dry seasons.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to the application of microorganisms in agricultural production, mainly the technology and application of an antibacterial and water-retaining organic fertilizer produced by a strain of Paenibacillus. technical background [0002] Bacillus ( Bacillus spp.) is a group of aerobic or facultatively anaerobic, spore-forming G + Generic term for bacilli. There are many kinds of Bacillus, which widely exist in nature and can be distributed in various ecological environments. They are the dominant populations of soil and plant rhizosphere microorganisms. Due to the characteristics of endophytic spores, strong stress resistance, fast reproduction speed, simple nutritional requirements and easy colonization in the root circle of plants, Bacillus has been widely studied and used in the biological control of plant diseases. The currently reported biocontrol bacillus are: Bacillus s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/04C12R1/07C05G3/80
Inventor 闫淑珍陈双林
Owner 常熟紫金知识产权服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com