Penthorum chinense pursh extract and preparation method and application thereof
A technology of pulling root vegetables and extracts, which is applied in the field of medicine, and achieves the effects of simple preparation method, reduced content and low cost
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Embodiment 1
[0016] Example 1. Preparation of root vegetable compounds
[0017] The root vegetables used in this experiment were collected in Chengdu, Sichuan.
[0018] 1. Preparation of Root Vegetable Extract
[0019] (1) 50 kg of dried whole herb of root-pulled vegetables, chopped and extracted with water according to conventional percolation, and the extract was concentrated under reduced pressure to 16 kg of alcohol-free extract. Then after being suspended in water, pass through a D101 macroporous resin column, and then eluted with water, 50% ethanol aqueous solution and 95% ethanol aqueous solution, respectively, and the eluent was concentrated under reduced pressure into an extract to obtain 3.5 kg of water-eluted partial extract, 50 % ethanol aqueous solution eluted partial extract 10kg and 95% ethanol aqueous solution eluted partial extract 2.5kg. Wherein the 50% ethanol aqueous solution eluted part of the extract is the root vegetable extract of the present invention (the same b...
Embodiment 2
[0022] Embodiment 2. preparation compound
[0023] Get 3.5g of the extractum of the 95% ethanol water elution part prepared in Example 1 (1), mix the sample with silica gel H (type 60, Qingdao Ocean Chemical Group Co., Ltd., the same below), put on the silica gel H chromatography column, and use Dichloromethane:methanol is 100:1→50:1→25:1→10:1→5:1→2:1→1:1 solvent system for gradient elution, and the eluents of each polarity are respectively Eluted five column volumes according to TLC (HSGF 254, the same below) detection, the developer is dichloromethane: methanol = 10: 1, and the ultraviolet lamp 254nm fluorescence color development, according to the different eluents, the collected eluents are divided into four parts, which are respectively concentrated under reduced pressure into immersion paste. The first part (the eluent is dichloromethane:methanol=100:1→50:1) extract 800mg is mixed with silica gel H, put on the silica gel H chromatography column, and use petroleum ether...
Embodiment 3
[0025] Example 3. Preparation of Compounds
[0026] Mix 3.5 g of the extracted extract of the 95% ethanol water elution part prepared in (2) in Example 1 with silica gel H to mix the sample, put on the silica gel H chromatography column, and use dichloromethane: methanol as 100: 1 → 50: 1 → The solvent system of 25:1→10:1→5:1→2:1→1:1 was used for gradient elution, and each polar eluent was eluted with five column volumes respectively. According to the TLC detection, the expanded The reagent is dichloromethane:methanol=10:1, and the ultraviolet lamp 254nm is used for fluorescence color development. The collected eluate is divided into four parts and concentrated under reduced pressure to form extracts. Mix 800 mg of the extract from the first part (dichloromethane:methanol is 100:1 → 50:1 eluent) with silica gel H, put the sample on the silica gel H chromatography column, and use petroleum ether:ethyl acetate=10:1 under normal pressure Isocratic constant-rate elution, accordin...
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