Preparation method of Bacillus licheniformis powder and microbial ecological agent

A technology of bacillus licheniformis and probiotics, applied in the field of preparation of bacillus licheniformis, can solve the problems of low production efficiency, high production cost, long production cycle, etc.

Active Publication Date: 2011-07-13
ZHEJIANG JINGXIN PHARMA
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] In order to solve the problems of few viable bacteria, long production cycle, high energy consumption, high production cost, low production ef

Method used

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  • Preparation method of Bacillus licheniformis powder and microbial ecological agent

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preparation example Construction

[0014] The preparation process of the present invention is as follows:

[0015] 1. Preparation of culture medium

[0016] (1) Nutrient broth medium: dissolve 1.0% peptone, 0.5% beef extract, and 0.5% sodium chloride in purified water, and adjust the pH to 7.0-7.4 with sodium hydroxide.

[0017] (2) Nutrient agar medium: dissolve 1.0% peptone, 0.5% beef extract, 0.5% sodium chloride, and 1.6% agar powder in purified water, and adjust the pH to 7.0-7.4 with sodium hydroxide.

[0018] 2. Recovery of Bacillus licheniformis freeze-dried bacteria

[0019] 2.1. Open the ampoule where the freeze-dried bacteria are stored (disinfect the outer wall of the bacteria tube before opening, generally use alcohol cotton with a concentration of 70%), and inject a small amount of nutrient broth medium (about 0.3ml) into it with a straw , and shake the freeze-dried bacteria suspension dissolved therein.

[0020] 2.2. Add an appropriate amount (about 50-100ml) of nutrient broth medium into a 20...

Embodiment 1

[0054] Example 1. Open a portion of 63516 strains of Bacillus licheniformis freeze-dried bacteria (its microorganism preservation number is CGMCC NO: 0183), which is equally divided into five parts and marked as A, B, C, D and E respectively.

[0055] Perform temperature gradient recovery culture on A, the temperature of each gradient stage is 25±1°C, 30±1°C, 37±1°C, and the corresponding culture time is 4h, 4h, 12h respectively; select three groups of dominant colonies with similar characteristics , marked as 1, 2 and 3 respectively, after the cultivation of the shake flask seed liquid and the fermentation culture time are not exactly the same, the fermented liquid is centrifuged to obtain the wet thallus; the wet thallus and anhydrous calcium carbonate The ratio is mixed and dried to make bacterial powder, and the results obtained are shown in Table 1. Wherein, the pH of the culture medium used in the cultivation process is 7.0-7.4.

[0056] Table 1:

[0057] Grou...

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Abstract

The invention relates to preparation and application of Bacillus licheniformis. In order to solve the problems of small obtained viable count, long production period, high energy consumption, high production cost, low production efficiency and large workload for culturing the Bacillus licheniformis in the prior art, the invention provides a new preparation method of Bacillus licheniformis powder and microbial ecological agent, comprising the following steps: firstly, carrying out temperature gradient anabiosis culture on the freeze-dried strain of the Bacillus licheniformis; then, breeding strains, fermenting, and decentralizing and drying to obtain bacterial powder; and preparing the microbial ecological agent by taking the bacterial powder as a raw material. The freeze-dried strain of the Bacillus licheniformis can be subjected to the temperature gradient anabiosis culture, and the freeze-dried strain can gradually adapt to the final culture temperature; and meanwhile, the activity of enzyme in the bacteria is activated so as to improve the metabolism speed of the bacteria and quicken the bacteria breeding speed, thereby improving the obtained viable count, shortening the production period and improving the production efficiency.

Description

technical field [0001] The invention relates to the preparation and application of Bacillus Licheniformis. Background technique [0002] The Chinese patent No. 92113318.9 (authorized announcement number CN 1044324C, authorized announcement date: July 28, 1999) discloses a kind of Bacillus licheniformis (Bacillus Licheniformis) 63516 strains (its microbial preservation number is CGMCC NO: 0183) ecological Preparation method of the preparation. The technical scheme for the preparation of the Bacillus licheniformis ecological preparation provided by this patent is: the bacterial species is cultivated in a broth medium with a pH of 7.2-7.4 for 18-24 hours to make a seed liquid, and the agar medium with a pH of 7.2-7.4 Expand the seed liquid for 48 hours to make a bacterial lawn liquid; collect the bacterial lawn liquid and put it into a centrifuge bottle for centrifugation to obtain a wet bacterial slime; add anhydrous calcium carbonate to the bacterial slime at a weight ratio ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74C12R1/10A61K35/742
Inventor 郑燕鹏张守真
Owner ZHEJIANG JINGXIN PHARMA
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